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181.
Takeshi Kobayashi Chihiro Taguchi Kakeru Kida Hiroko Matsuda Takeshi Terahara Chiaki Imada Nant Kay Thwe Moe Su Myo Thwe 《World journal of microbiology & biotechnology》2016,32(10):166
The distribution and characterization of bacteria including lactic acid bacteria (LAB) in the traditional and popular salted fish yegyo ngapi in Myanmar were studied to clarify the contribution of these bacteria to the curing and ripening of this product. Samples of yegyo ngapi purchased from a market in Yangon were used. Most of the isolates obtained using de Man, Rogosa and Sharpe medium containing 10 % NaCl were identified as coccoid LAB on the basis of their basic phenotypic characteristics. From the results of 16S rRNA gene sequencing and PCR-restriction fragment length polymorphism analysis of this gene, most of the isolates were identified as the halophilic LAB Tetragenococcus muriaticus. Analyses of the 16S rRNA gene based on the clone library using DNA extracted from salted fish products were also performed. The results of these molecular-analysis-based techniques showed that spore-forming and non-spore-forming anaerobic bacteria including the genera Clostridium and Halanaerobium in addition to T. muriaticus were also frequently found in bacterial communities. These findings suggest that the anaerobic condition during curing and ripening resulted in bacterial communities composed of strictly anaerobic bacteria and halophilic LAB, and that these bacteria might also contribute to the manufacturing processes of this product. In addition, DNA sequences similar to that of Clostridium botulinum were found in the clone library analysis. Therefore, despite no reports of botulism poisoning from the region where the samples were taken, closer surveillance should be carried out from the viewpoint of food safety. 相似文献
182.
Reiko Ando Noriyo Tokuda Tokunori Yamamoto Kazutaka Ikeda Noboru Hashimoto Ryo Taguchi Xiaoen Fan Keiko Furukawa Yukio Niimura Akemi Suzuki Momokazu Goto Koichi Furukawa 《Glycoconjugate journal》2016,33(2):169-180
In this study, we immunized Gb3/CD77 synthase gene (A4galt) knockout (KO) mice with glycosphingolipids (GSLs) extracted from 3 renal cell cancer (RCC) cell lines to raise monoclonal antibodies (mAbs) reactive with globo-series GSLs specifically expressed in RCCs. Although a number of mAbs reactive with globo-series GSLs were generated, they reacted with both RCC cell lines and normal kidney cells. When we analyzed recognized antigens by mAbs that were specifically reactive with RCC, but not with normal kidney cells at least on the cell surface, many of them turned out to be reactive with sulfoglycolipids. Eight out of 11 RCC-specific mAbs were reactive with SM2 alone, and the other 3 mAbs were more broadly reactive with sulfated glycolipids, i.e. SM3 and SM4 as well as SM2. In the immunohistochemistry, these anti-sulfoglycolipids mAbs showed RCC-specific reaction, with no or minimal reaction with adjacent normal tissues. Thus, immunization of A4galt KO mice with RCC-derived GSLs resulted in the generation of anti sulfated GSL mAbs, and these mAbs may be applicable for the therapeutics for RCC patients. 相似文献
183.
A Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon the S(1)-to-S(2) transition was obtained with Ca(2+)-depleted photosystem II (PSII) membranes to investigate the structural relevance of Ca(2+) to the Mn cluster. Previously, Noguchi et al. [Biochim. Biophys. Acta 1228 (1995) 189] observed drastic changes in the carboxylate stretching region of the S(2)/S(1) FTIR spectrum upon Ca(2+) depletion, whereas Kimura and co-workers [Biochemistry 40 (2001) 14061; ibid. 41 (2002) 5844] later claimed that these changes were not ascribed to Ca(2+) depletion itself but caused by the interaction of EDTA to the Mn cluster and/or binding of K(+) at the Ca(2+) site. In the present study, the preparation of the Ca(2+)-depleted PSII sample and its FTIR measurement were performed in the absence of EDTA and K(+). The obtained S(2)/S(1) spectrum exhibited the loss of carboxylate bands at 1587/1562 and 1364/1403 cm(-1) and diminished amide I intensities, which were identical to the previous observations in the presence of EDTA and K(+). This result indicates that the drastic FTIR changes are a pure effect of Ca(2+) depletion, and provides solid evidence for the general view that Ca(2+) is strongly coupled with the Mn cluster. 相似文献
184.
Terasaki N Yamamoto N Tamada K Hattori M Hiraga T Tohri A Sato I Iwai M Iwai M Taguchi S Enami I Inoue Y Yamanoi Y Yonezawa T Mizuno K Murata M Nishihara H Yoneyama S Minakata M Ohmori T Sakai M Fujii M 《Biochimica et biophysica acta》2007,1767(6):653-659
We report on the first successful output of electrons directly from photosystem I (PSI) of thermophilic cyanobacteria to the gate of a field-effect transistor (FET) by bypassing electron flow via a newly designed molecular wire, i.e., artificial vitamin K(1), and a gold nanoparticle; in short, this newly manufactured photosensor employs a bio-functional unit as the core of the device. Photo-electrons generated by the irradiation of molecular complexes composed of reconstituted PSI on the gate were found to control the FET. This PSI-bio-photosensor can be used to interpret gradation in images. This PSI-FET system is moreover sufficiently stable for use exceeding a period of 1 year. 相似文献
185.
Nao Terasaki Kaoru Tamada Takashi Hiraga Akihiko Tohri Masako Iwai Shunpei Taguchi Yasunori Inoue Yoshinori Yamanoi Katsuya Mizuno Hiroshi Nishihara Satoshi Yoneyama Tsutomu Ohmori Masaaki Fujii 《BBA》2007,1767(6):653-659
We report on the first successful output of electrons directly from photosystem I (PSI) of thermophilic cyanobacteria to the gate of a field-effect transistor (FET) by bypassing electron flow via a newly designed molecular wire, i.e., artificial vitamin K1, and a gold nanoparticle; in short, this newly manufactured photosensor employs a bio-functional unit as the core of the device. Photo-electrons generated by the irradiation of molecular complexes composed of reconstituted PSI on the gate were found to control the FET. This PSI-bio-photosensor can be used to interpret gradation in images. This PSI-FET system is moreover sufficiently stable for use exceeding a period of 1 year. 相似文献
186.
Kazunori Taguchi Yoshihiro Aoyagi Hiromi Matsusaki Toshiaki Fukui Yoshiharu Doi 《Biotechnology letters》1999,21(7):579-584
A Pseudomonas sp. 61-3 malonyl-CoA-ACP transacylase gene (fabD
Ps) was cloned by Southern analysis using an equivalent gene of Escherichia coli (fabD
Ec) as a probe. Some recombinant E. coli HB101 strains harboring fabD
Ps, fabD
Ec, or E. coli 3-ketoacyl-ACP synthase III gene (fabH
Ec) with Aeromonas caviae polyhydroxyalkanoate synthase gene (phaC
Ac) were constructed and grown on one-stage cultivation in Luria-Bertani broth containing glucose as carbon source. These strains accumulated 5 to 11 wt% of poly (3-hydroxybutyrate) (PHB) within cells. Over-expression of fabH
Ec, fabD
Ec, or fabD
Ps has been suggested to lead the monomer-supply of (R)-3-hydroxybutyryl-CoA for PHB synthesis in E. coli cells. 相似文献
187.
Short communication. Biomass and production of cyanobacteria in a coastal water of Sagami Bay, Japan
Hamasaki K; Satoh F; Kikuchi T; Toda T; Taguchi S 《Journal of plankton research》1999,21(8):1583-1591
Cyanobacteria were relatively small contributors to carbon biomass
(097-18%) in the euphotic zone. However, a higher contribution to
production obtained at the surface water (16-45%) implies that they
contribute more to carbon cycling than is expected from their biomass
相似文献
188.
M. Okochi T. Taguchi M. Tsuboi N. Nakamura T. Matsunaga 《Applied microbiology and biotechnology》1999,51(3):364-369
A rapid method for the direct measurement of viable and dead adhering diatoms was developed using a fluorescent dye, TO-PRO-1
iodide. By staining the marine diatom, Nitzchia closterium, with TO-PRO-1 iodide, viable and dead cells were identified as red and yellow cells respectively, under an epifluorescence
microscope employing blue excitation. Only dead cells were stained with TO-PRO-1 iodide. Viable cells were observed as red
because of autofluorescence arising from intracellular chlorophyll, whereas dead cells were observed as yellow because of
the fluorescence of TO-PRO-1 iodide. The percentage of TO-PRO-1-iodide-stained was correlated with the percentage of dead
cells in N. closterium cells exposed to heat (60 °C, 15 min). Other microalgae containing intracellular chlorophyll could be also distinguished
as viable or dead cells by this fluorometric staining method. This method was applied for the assessment of N. closterium cells killed by the electrochemical treatment and used to monitor biofouling populations and their viability directly on
the electrode surface. When 1.0 V was applied against a saturated calomel electrode, 99% of the cells attached to graphite
electrode were killed in 1 h.
Received: 7 August 1998 / Received revision: 16 October 1998 / Accepted: 7 November 1998 相似文献
189.
Suguru N. Kudoh Kazuyuki Kiyosue Michiki Kasai Takahisa Taguchi 《Cellular and molecular neurobiology》1999,19(5):575-585
1.We reported in a previous paper that long-lasting enhancement of spontaneous excitatory post synaptic currents (SEPSCs) in cultured chick cerebral neurons was induced by exposure to a conditioned medium (CM) prepared by Mg2+-free treatment of neurons. This suggested that the CM contained a diffusible factor(s) for the potentiation.2.In this paper, the factor(s) was shown to be a protein(s) by heat and trypsin treatment of the CM.3.The factor induced the potentiation within 5 min, but it was not required for maintenance of increased SEPSCs.4.The factors in CM induced the potentiation without protein synthesis.5.Protein synthesis at least in postsynaptic neurons, was indispensable to induce the potentiation by the Mg2+-free condition. 相似文献
190.