全文获取类型
收费全文 | 171篇 |
免费 | 6篇 |
出版年
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 4篇 |
2014年 | 6篇 |
2013年 | 10篇 |
2012年 | 10篇 |
2011年 | 7篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 5篇 |
2007年 | 11篇 |
2006年 | 11篇 |
2005年 | 12篇 |
2004年 | 6篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 1篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 7篇 |
1993年 | 3篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有177条查询结果,搜索用时 140 毫秒
171.
Biopolymer flocculant produced by an Pseudomonas sp. 总被引:13,自引:0,他引:13
Haruhiko Yokoi Takashi Yoshida Jun Hirose Sachio Hayashi Yoshiyuki Takasaki 《Biotechnology Techniques》1998,12(7):511-514
A biopolymer flocculant produced by Pseudomonas sp. A-99 had flocculating activity both in inorganic suspensions containing Ca2, Mg2 or Fe3 and in organic suspensions containing Fe2, Fe3 or Al3. The flocculant was an acidic protein and contained a small amount of an acidic polysaccharide consisting of galacturonic acid, glucose and galactose. Productivity of the flocculant was about 450 mg/l medium. © Rapid Science Ltd. 1998 相似文献
172.
The 210th lysine (K210) at the active site in Saccharomycopsis fibuligera alpha-amylase was altered to arginine (R) or asparagine (N) by site-directed mutagenesis. Replacement of K210 by R strengthened the 7th and weakened the 8th subsite affinities. K210 was found to contribute to both the 8th and the 7th subsites. The catalytic activity of the K210R enzyme for the hydrolysis of maltose (G2) was three-times higher than that of the native enzyme due to an increase in the affinity of the 7th subsite adjacent to the catalytic site, whereas the activity of the K210N enzyme for G2 was decreased to 1% of that of the native enzyme by a reduction in the 7th subsite affinity. 相似文献
173.
174.
Haruhiko Yokoi Shingo Mori Jun Hirose Sachio Hayashi Yoshiyuki Takasaki 《Biotechnology letters》1998,20(9):895-899
A photosynthetic bacterium having ability to produce H2 from acetic, butyric and lactic acids, Rhodobacter sp. M-19 was isolated. H2 was produced from starch in a batch culture by Clostridium butyricum and in a two-step batch culture by C. butyricum and Rhodobacter sp. M-19 in yields of 1.9 and 3.6 mol H2/mol glucose, respectively. A mixed culture of C. butyricum and Rhodobacter sp. M-19 produced H2 from starch with a yield of 6.6 mol H2/mol glucose in a fed-batch culture. © Rapid Science Ltd. 1998 相似文献
175.
H2 production from starch by a mixed culture of Clostridium butyricum and Enterobacter aerogenes 总被引:6,自引:0,他引:6
Haruhiko Yokoi Tadafumi Tokushige Jun Hirose Sachio Hayashi Yoshiyuki Takasaki 《Biotechnology letters》1998,20(2):143-147
A mixed continuous culture of Clostridium butyricum and Enterobacter aerogenes removed O2 in a reactor and produced H2 from starch with yield of more than 2 mol H2/mol glucose without any reducing agents in the medium. Co-immobilized cells of the bacteria on porous glass beads evolved H2 from starch at 1.3 l/l.h, with H2 yield of 2.6 mol H2/ mol glucose at dilution rate of 1.0 h–1 in a continuous culture. 相似文献
176.
Ca2+-sensitizing effects of the mutations at Ile-79 and Arg-92 of troponin T in hypertrophic cardiomyopathy 总被引:1,自引:0,他引:1
Morimoto Sachio; Yanaga Fumi; Minakami Reiko; Ohtsuki Iwao 《American journal of physiology. Cell physiology》1998,275(1):C200
Several mutationsin human cardiac troponin T (TnT) gene have been reported to causehypertrophic cardiomyopathy (HCM). To explore the effects of themutations on cardiac muscle contractile function under physiologicalconditions, human cardiac TnT mutants, Ile79Asn and Arg92Gln, as wellas wild type, were expressed in Escherichiacoli and exchanged into permeabilized rabbit cardiac muscle fibers, and Ca2+-activatedforce was determined. The freeCa2+ concentrations required fortension generation were found to be significantly lower in the mutantTnT-exchanged fibers than in the wild-type TnT-exchanged fibers,whereas no significant differences were found in tension-generatingcapability under maximal activating conditions and in cooperativity.These results suggest that a heightenedCa2+ sensitivity of cardiac musclecontraction is one of the factors to cause HCM associated with theseTnT mutations. 相似文献
177.
Kazuo Iida Akio Abe Hidenori Matsui Hirofumi Danbara Sachio Wakayama Kazuyoshi Kawahara 《FEMS microbiology letters》1993,114(2):167-172
Abstract Cell-free extracts of Thiobacillus acidophilus catalysed the stoichiometric conversion of tetrathionate to thiosulphate, sulphur and two protons. The pH optimum of the enzyme activity was 3.0 and its temperature optimum 40°C. The enzyme was unstable at 30 and 40°C, at which its activity decreased to zero within 100 and 20 h, respectively. Enzyme activity was not affected by incubation for 1 week on ice or by freezing and thawing of the extract. The K m for tetrathionate was 0.3 mM. Enzyme activity was stimulated by ammonium sulphate up to a concentration of 1M. The results indicate that trithionate hydrolase cannot account for the observed conversion of tetrathionate. 相似文献