排序方式: 共有427条查询结果,搜索用时 271 毫秒
401.
Wu HS Salzberg SP Gil J 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》2006,28(6):307-315
OBJECTIVE: To identify extracellular matrix deposition on combined Masson elastin stains from cross-sectional, fixed vein grafts. STUDY DESIGN: Source vectors from RGB components of color images are transformed into new vectors with most of the energy concentrated in fewer coefficients based on the eigenvalues and eigenvectors of their co-variance matrix so their dimension can be reduced for efficient computation and analysis. The vectors are distributed in a triangular shape in which most vectors are located in a long, narrow strip that can be approximated by a straight line while a separate group of vectors from collagen areas form a loose cluster away from the line. An iterative procedure has been developed for the representative vectors in the 2 centroids for linear and circular clusters. The linear centroid consists of all vectors in a straight line, and the centroid of the circular cluster is a single vector. Vector classification is based on the measure of its distance to each of the 2 centroids. RESULTS: The automatic segmentation of the collagen content pixels in green-blue matches the image background color. CONCLUSION: The procedure automatically quantifies and characterizes the neointimal deposition after surgical vein grafting in mice. 相似文献
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Kevin K.-C. Liu Shubha Bagrodia Simon Bailey Hengmiao Cheng Hui Chen Lisa Gao Samantha Greasley Jacqui E. Hoffman Qiyue Hu Ted O. Johnson Dan Knighton Zhengyu Liu Matthew A. Marx Mitchell D. Nambu Sacha Ninkovic Bernadette Pascual Kristina Rafidi Caroline M.-L. Rodgers Graham L. Smith Shaoxian Sun Aihua Zou 《Bioorganic & medicinal chemistry letters》2010,20(20):6096-6099
Pteridinones were designed based on a non-selective kinase template. Because of the uniqueness of the PI3K and mTOR binding pockets, a methyl group was introduced to C-4 position of the peteridinone core to give compounds with excellent selectivity for PI3K and mTOR. This series of compounds were further optimized to improve their potency against PI3Kα and mTOR. Finally, orally active compounds with improved solubility and robust in vivo efficacy in tumor growth inhibition were identified as well. 相似文献
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Nesvizhskii AI Roos FF Grossmann J Vogelzang M Eddes JS Gruissem W Baginsky S Aebersold R 《Molecular & cellular proteomics : MCP》2006,5(4):652-670
In mass spectrometry-based proteomics, frequently hundreds of thousands of MS/MS spectra are collected in a single experiment. Of these, a relatively small fraction is confidently assigned to peptide sequences, whereas the majority of the spectra are not further analyzed. Spectra are not assigned to peptides for diverse reasons. These include deficiencies of the scoring schemes implemented in the database search tools, sequence variations (e.g. single nucleotide polymorphisms) or omissions in the database searched, post-translational or chemical modifications of the peptide analyzed, or the observation of sequences that are not anticipated from the genomic sequence (e.g. splice forms, somatic rearrangement, and processed proteins). To increase the amount of information that can be extracted from proteomic MS/MS datasets we developed a robust method that detects high quality spectra within the fraction of spectra unassigned by conventional sequence database searching and computes a quality score for each spectrum. We also demonstrate that iterative search strategies applied to such detected unassigned high quality spectra significantly increase the number of spectra that can be assigned from datasets and that biologically interesting new insights can be gained from existing data. 相似文献
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Sacha JB Reynolds MR Buechler MB Chung C Jonas AK Wallace LT Weiler AM Lee W Piaskowski SM Soma T Friedrich TC Wilson NA Watkins DI 《Journal of virology》2008,82(18):9293-9298
The kinetics of peptide presentation by major histocompatibility complex class I (MHC-I) molecules may contribute to the efficacy of CD8+ T cells. Whether all CD8+ T-cell epitopes from a protein are presented by the same MHC-I molecule with similar kinetics is unknown. Here we show that CD8+ T-cell epitopes derived from SIVmac239 Gag are presented with markedly different kinetics. We demonstrate that this discrepancy in presentation is not related to immunodominance but instead is due to differential requirements for epitope generation. These results illustrate that significant differences in presentation kinetics can exist among CD8+ T-cell epitopes derived from the same viral protein. 相似文献
407.
With the available Arabidopsis genome and near-completion of the rice genome sequencing project, large-scale analysis of plant proteins with mass spectrometry has now become possible. Determining the proteome of a cell is a challenging task, which is complicated by proteome dynamics and complexity. The biochemical heterogeneity of proteins constrains the use of standardized analytical procedures and requires demanding techniques for proteome analysis. Several proteome studies of plant cell organelles have been reported, including chloroplasts and mitochondria. Chloroplasts are of particular interest for plant biologists because of their complex biochemical pathways for essential metabolic functions. Information from the chloroplast proteome will therefore provide new insights into pathway compartmentalization and protein sorting. Some approaches for the analysis of the chloroplast proteome and future prospects of plastid proteome research are discussed here. 相似文献
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Carmen Dorca‐Fornell Radoslaw Pajor Christoph Lehmeier Marísa Pérez‐Bueno Marion Bauch Jen Sloan Colin Osborne Stephen Rolfe Craig Sturrock Sacha Mooney Andrew Fleming 《The Plant journal : for cell and molecular biology》2013,76(6):914-929
The causal relationship between cell division and growth in plants is complex. Although altered expression of cell‐cycle genes frequently leads to altered organ growth, there are many examples where manipulation of the division machinery leads to a limited outcome at the level of organ form, despite changes in constituent cell size. One possibility, which has been under‐explored, is that altered division patterns resulting from manipulation of cell‐cycle gene expression alter the physiology of the organ, and that this has an effect on growth. We performed a series of experiments on retinoblastoma‐related protein (RBR), a well characterized regulator of the cell cycle, to investigate the outcome of altered cell division on leaf physiology. Our approach involved combination of high‐resolution microCT imaging and physiological analysis with a transient gene induction system, providing a powerful approach for the study of developmental physiology. Our investigation identifies a new role for RBR in mesophyll differentiation that affects tissue porosity and the distribution of air space within the leaf. The data demonstrate the importance of RBR in early leaf development and the extent to which physiology adapts to modified cellular architecture resulting from altered cell‐cycle gene expression. 相似文献
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Charlotte Pandiani Thomas Strub Nicolas Nottet Yann Cheli Giovanni Gambi Karine Bille Chrystel Husser Mlanie Dalmasso Guillaume Branger Sandra Lassalle Virginie Magnone Florence Pdeutour Marie Irondelle Clia Maschi Sacha Nahon-Estve Arnaud Martel Jean-Pierre Caujolle Paul Hofman Kvin LeBrigand Irwin Davidson Stphanie Baillif Pascal Barbry Robert Ballotti Corine Bertolotto 《Cell death and differentiation》2021,28(6):1990