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Socioeconomic status and anthropometric changes—A meta‐analytic approach from seven German cohorts 下载免费PDF全文
Johannes Haerting Saskia Hartwig Daniel Tiller Daniel Medenwald Susanne Vogt Barbara Thorand Rolf Holle Ursula Bachlechner Heiner Boeing Benedikt Merz Ute Nöthlings Sabrina Schlesinger Sabine Schipf Till Ittermann Nicole Aumann Anja Schienkiewitz Marjolein Haftenberger Karin H. Greiser Jasmine Neamat‐Allah Verena Katzke Alexander Kluttig 《Obesity (Silver Spring, Md.)》2016,24(3):710-718
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Angiopoietin‐2, its soluble receptor Tie‐2, and metabolic syndrome components in a population‐based sample 下载免费PDF全文
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Stephan Zindel Vera Ehret Marina Ehret Madeleine Hentschel Samantha Witt Andreas Kr?mer David Fiebig Norbert Jüttner Sabrina Fr?ls Felicitas Pfeifer Hans-Lothar Fuchsbauer 《PloS one》2016,11(2)
Streptomyces mobaraensis DSM 40847 secretes transglutaminase that cross-links proteins via γ-glutamyl-ε-lysine isopeptide bonds. Characterized substrates are inhibitory proteins acting against various serine, cysteine and metalloproteases. In the present study, the bacterial secretome was examined to uncover additional transglutaminase substrates. Fractional ethanol precipitation of the exported proteins at various times of culture growth, electrophoresis of the precipitated proteins, and sequencing of a 39 kDa protein by mass spectrometry revealed the novel beta-lactamase Sml-1. As indicated by biotinylated probes, Sml-1, produced in E. coli, exhibits glutamine and lysine residues accessible for transglutaminase. The chromogenic cephalosporin analogue, nitrocefin, was hydrolyzed by Sml-1 with low velocity. The obtained Km and kcat values of the recombinant enzyme were 94.3±1.8 μM and 0.39±0.03 s-1, respectively. Penicillin G and ampicillin proved to be weak inhibitors of nitrocefin hydrolysis (Ki of 0.1 mM and 0.18 mM). Negligible influence of metals on β-lactamase activity ruled out that Sml-1 is a Zn2+-dependent class B beta-lactamase. Rather, sequence motifs such as SITK, YSN, and HDG forming the active core in a hypothetical structure may be typical for class C beta-lactamases. Based on the results, we assume that the novel transglutaminase substrate ensures undisturbed growth of aerial hyphae in Streptomyces mobaraensis by trapping and inactivating hostile beta-lactam antibiotics. 相似文献
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Sabrina Rasheed A. K. Siddique Tamanna Sharmin A. M. R. Hasan S. M. A. Hanifi M. Iqbal Abbas Bhuiya 《PloS one》2016,11(4)
BackgroundHigh salt consumption is an important risk factor of elevated blood pressure. In Bangladesh about 20 million people are at high risk of hypertension due to climate change induced saline intrusion in water. The objective of this study is to assess beliefs, perceptions, and practices associated with salt consumption in coastal Bangladesh.MethodsThe study was conducted in Chakaria, Bangladesh between April-June 2011. It was a cross sectional mixed method study. For the qualitative study 6 focus group discussions, 8 key informant interviews, 60 free listing exercises, 20 ranking exercises and 10 observations were conducted. 400 adults were randomly selected for quantitative survey. For analysis we used SPSS for quantitative data, and Anthropac and Nvivo for qualitative data.ResultsSalt was described as an essential component of food with strong cultural and religious roots. People described both health benefits and risks related to salt intake. The overall risk perception regarding excessive salt consumption was low and respondents believed that the cooking process can render the salt harmless. Respondents were aware that salt is added in many foods even if they do not taste salty but did not recognize that salt can occur naturally in both foods and water.ConclusionsIn the study community people had low awareness of the risks associated with excess salt consumption and salt reduction strategies were not high in their agenda. The easy access to and low cost of salt as well as unrecognised presence of salt in drinking water has created an environment conducive to excess salt consumption. It is important to design general messages related to salt reduction and test tailored strategies especially for those at high risk of hypertension. 相似文献
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Andreas Hartmann Julia Muellner Niklaus Meier Helke Hesekamp Priscilla van Meerbeeck Marie-Odile Habert Aurélie Kas Marie-Laure Tanguy Merry Mazmanian Hervé Oya Nissen Abuaf Hafida Gaouar Sabrina Salhi Fanny Charbonnier-Beaupel Marie-Hélène Fievet Damien Galanaud Sophie Arguillere Emmanuel Roze Bertrand Degos David Grabli Lucette Lacomblez Cécile Hubsch Marie Vidailhet Anne-Marie Bonnet Jean-Christophe Corvol Michael Schüpbach 《PloS one》2016,11(9)
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Sabrina I?igo Astrid Nagels Durand Andrés Ritter Sabine Le Gall Martin Termathe Roland Klassen Takayuki Tohge Barbara De Coninck Jelle Van Leene Rebecca De Clercq Bruno P.A. Cammue Alisdair R. Fernie Kris Gevaert Geert De Jaeger Sebastian A. Leidel Raffael Schaffrath Mieke Van Lijsebettens Laurens Pauwels Alain Goossens 《Plant physiology》2016,172(2):858-873