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371.
Bahram Abdollahi Nejand Ihteaz M. Hossain Marius Jakoby Somayeh Moghadamzadeh Tobias Abzieher Saba Gharibzadeh Jonas A. Schwenzer Pariya Nazari Fabian Schackmar Dirk Hauschild Lothar Weinhardt Uli Lemmer Bryce S. Richards Ian A. Howard Ulrich W. Paetzold 《Liver Transplantation》2020,10(5)
All‐perovskite multijunction photovoltaics, combining a wide‐bandgap (WBG) perovskite top solar cell (EG ≈1.6–1.8 eV) with a low‐bandgap (LBG) perovskite bottom solar cell (EG < 1.3 eV), promise power conversion efficiencies (PCEs) >33%. While the research on WBG perovskite solar cells has advanced rapidly over the past decade, LBG perovskite solar cells lack PCE as well as stability. In this work, vacuum‐assisted growth control (VAGC) of solution‐processed LBG perovskite thin films based on mixed Sn–Pb perovskite compositions is reported. The reported perovskite thin films processed by VAGC exhibit large columnar crystals. Compared to the well‐established processing of LBG perovskites via antisolvent deposition, the VAGC approach results in a significantly enhanced charge‐carrier lifetime. The improved optoelectronic characteristics enable high‐performance LBG perovskite solar cells (1.27 eV) with PCEs up to 18.2% as well as very efficient four‐terminal all‐perovskite tandem solar cells with PCEs up to 23%. Moreover, VAGC leads to promising reproducibility and potential in the fabrication of larger active‐area solar cells up to 1 cm2. 相似文献
372.
Fatemeh Firouzi Amoodizaj Sevda Baghaeifar Elham Taheri Mahdieh Farhoudi Sefidan Jadid Maryam Safi Nasrin Seyyed Sani Saba Hajazimian Alireza Isazadeh Dariush Shanehbandi 《Journal of biochemical and molecular toxicology》2020,34(6)
Gastric cancer (GC) is one of the prevalent human malignancies and the third most common cause of cancer‐related death worldwide. The doxorubicin hydrochloride is one of the important chemotherapeutic anticancer agents, with a limited therapeutic efficacy for treatment of GC. Therefore, taking advantage of synergistic effects by strategies like combination therapy seems appropriate and promising in treatment of GC. The aim of this study was to investigate a novel method to enhance the therapeutic efficacy of doxorubicin (as a chemotherapeutic agent) by co‐administration of curcumin (as a bioactive herbal compound) in GC treatment. In the present study, the effects of curcumin, doxorubicin, and their combinations (Dox‐Cur) were evaluated on the viability, morphological features, tumor spheroid formation, migration, invasion, and apoptosis of gastric adenocarcinoma cell line (AGS). Moreover, expression levels of BAX, BCL‐2, and CASP9 genes were assessed among AGS cells treated with curcumin, doxorubicin, and Dox‐Cur. The obtained results showed that all of curcumin, doxorubicin, and Dox‐Cur treatments significantly decreased the viability, tumor spheroid formation, migration, and invasion in the GC model cells. Furthermore, apoptosis rates in AGS cells were increased in a concentration‐ and time‐dependent manner in all of the treatment groups. Moreover, the anticancer activity of the Dox‐Cur combination was significantly more than curcumin and doxorubicin treatments alone. According to the results, Dox‐Cur combination therapy exerts more profound apoptotic and anticancer effects on the AGS cell line than curcumin or doxorubicin monotherapy. 相似文献
373.
Perrine Pennamen Angle Tingaud‐Sequeira Vincent Michaud Fanny Morice‐Picard Claudio Plaisant Catherine Vincent‐Delorme Fabienne Giuliano Saba Azarnoush Yline Capri Carolina Maron Didier Lacombe Eulalie Lasseaux Benoît Arveiler 《Pigment cell & melanoma research》2021,34(1):132-135
Hermansky–Pudlak syndrome (HPS) associates oculocutaneous albinism and systemic affections including platelet dense granules anomalies leading to bleeding diathesis and, depending on the form, pulmonary fibrosis, immunodeficiency, and/or granulomatous colitis. So far, 11 forms of autosomal recessive HPS caused by pathogenic variants in 11 different genes have been reported. We describe three HPS‐8 consanguineous families with different homozygous pathogenic variants in BLOC1S3 (NM_212550.3), one of which is novel. These comprise two deletions leading to a reading frameshift (c.385_403del, c.338_341del) and one in frame deletion (c.444_467del). All patients have moderate oculocutaneous albinism and bleeding diathesis, but other HPS symptoms are not described. One patient diagnosed with HPS‐8 suffered from lymphocyte‐predominant Hodgkin lymphoma. The mild severity of HPS‐8 is consistent with other HPS forms caused by variants in BLOC‐1 complex coding genes (HPS‐7, DTNBP1; HPS‐9, BLOC1S6, HPS‐11, BLOC1S5). 相似文献
374.
375.
Saba Tufail Mohammad Owais Shadab Kazmi Renu Balyan Jasneet Kaur Khalsa Syed Mohd. Faisal Mohd. Asif Sherwani Manzoor Ahmad Gatoo Mohd. Saad Umar Swaleha Zubair 《The Journal of biological chemistry》2015,290(7):4131-4148
Amyloids are highly organized protein aggregates that arise from inappropriately
folded versions of proteins or polypeptides under both physiological as well as
simulated ambiences. Once thought to be irreversible assemblies, amyloids have begun
to expose their more dynamic and reversible attributes depending upon the intrinsic
properties of the precursor protein/peptide and experimental conditions such as
temperature, pressure, structural modifications in proteins, or presence of chemicals
in the reaction mixture. It has been repeatedly proposed that amyloids undergo
transformation to the bioactive peptide/protein forms under specific conditions. In
the present study, amyloids assembled from the model protein ovalbumin (OVA) were
found to release the precursor protein in a slow and steady manner over an extended
time period. Interestingly, the released OVA from amyloid depot was found to exhibit
biophysical characteristics of native protein and reacted with native-OVA specific
monoclonal as well as polyclonal antibodies. Moreover, antibodies generated upon
immunization of OVA amyloidal aggregates or fibrils were found to recognize the
native form of OVA. The study suggests that amyloids may act as depots for the native
form of the protein and therefore can be exploited as vaccine candidates, where slow
antigen release over extended time periods is a pre-requisite for the development of
desired immune response. 相似文献
376.
Sathya Narayanan Nagarajan Sandeep Upadhyay Yogesh Chawla Shazia Khan Saba Naz Jayashree Subramanian Sheetal Gandotra Vinay Kumar Nandicoori 《The Journal of biological chemistry》2015,290(15):9626-9645
The essential mycobacterial protein kinases PknA and PknB play crucial roles in modulating cell shape and division. However, the precise in vivo functional aspects of PknA have not been investigated. This study aims to dissect the role of PknA in mediating cell survival in vitro as well as in vivo. We observed aberrant cell shape and severe growth defects when PknA was depleted. Using the mouse infection model, we observe that PknA is essential for survival of the pathogen in the host. Complementation studies affirm the importance of the kinase, juxtamembrane, and transmembrane domains of PknA. Surprisingly, the extracytoplasmic domain is dispensable for cell growth and survival in vitro. We find that phosphorylation of the activation loop at Thr172 of PknA is critical for bacterial growth. PknB has been previously suggested to be the receptor kinase, which activates multiple kinases, including PknA, by trans-phosphorylating their activation loop residues. Using phospho-specific PknA antibodies and conditional pknB mutant, we find that PknA autophosphorylates its activation loop independent of PknB. Fluorescently tagged PknA and PknB show distinctive distribution patterns within the cell, suggesting that although both kinases are known to modulate cell shape and division, their modes of action are likely to be different. This is supported by our findings that expression of kinase-dead PknA versus kinase-dead PknB in mycobacterial cells leads to different cellular phenotypes. Data indicate that although PknA and PknB are expressed as part of the same operon, they appear to be regulating cellular processes through divergent signaling pathways. 相似文献
377.
The development of an immune response to self antigens drives naive T cells to differentiate into subsets of CD8(+) and CD4(+) effector cells including T(H)1, T(H)2, cells and the more recently described T(H)17, and regulatory T cells (T(reg)). Rheumatoid arthritis is an autoimmune disease that engages an uncontrolled influx of inflammatory cells to the joints, eventually leading to joint damage. The role that effector T cells play in the local or systemic maintenance of, or protection against, inflammation and subsequent joint damage is now becoming better understoodthrough the use of animal models. In this review, we will explore the different animal models of RA, and their contribution to elucidating the role that effector T cells play in the regulation, induction, and maintenance of inflammatory joint disease. This understanding will aid in the design of more effective therapeutic strategies for rheumatoid arthritis and other autoimmune disorders. 相似文献
378.
379.
Wnt proteins comprise a large family of secreted ligands implicated in a wide variety of biological roles. WntD has previously been shown to inhibit the nuclear accumulation of Dorsal/NF-κB protein during embryonic dorsal/ventral patterning and the adult innate immune response, independent of the well-studied Armadillo/β-catenin pathway. In this paper, we present a novel phenotype for WntD mutant embryos, suggesting that this gene is involved in migration of primordial germ cells (PGC) to the embryonic gonad. Additionally, we describe a genetic suppressor/enhancer screen aimed at identifying genes required for WntD signal transduction, based on the previous observation that maternal overexpression of WntD results in lethally dorsalized embryos. Using an algorithm to narrow down our hits from the screen, we found two novel WntD signaling components: Fz4, a member of the Frizzled family, and the Drosophila Ceramide Kinase homolog, Dcerk. We show here that Dcerk and Dmulk (Drosophila Multi-substrate lipid kinase) redundantly mediate PGC migration. Our data are consistent with a model in which the activity of lipid phosphate phosphatases shapes a concentration gradient of ceramide-1-phosphate (C1P), the product of Dcerk, allowing proper PGC migration. 相似文献
380.
Alvarez-Rueda N Desselle A Cochonneau D Chaumette T Clemenceau B Leprieur S Bougras G Supiot S Mussini JM Barbet J Saba J Paris F Aubry J Birklé S 《PloS one》2011,6(9):e25220