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41.
Transient receptor potential ion channel melastatin subtype 8 (TRPM8) is activated by cold temperature and cooling agents,
such as menthol and icilin. Compounds containing peppermint are reported to reduce symptoms of environmental cold stress such
as cold allodynia in dorsal root ganglion (DRG) neuron; however, the underlying mechanisms of action are unclear. We tested
the effects of physiological heat (37°C), anthralic acid (ACA and 0.025 mM), 2-aminoethyl diphenylborinate (2-APB and 0.05)
on noxious cold (10°C) and menthol (0.1 mM)-induced TRPM8 cation channel currents in the DRG neurons of rats. DRG neurons
were freshly isolated from rats. In whole-cell patch clamp experiments, TRPM8 currents were consistently induced by noxious
cold or menthol. TRPM8 channels current densities of the neurons were higher in cold and menthol groups than in control. When
the physiological heat is introduced by chamber TRPM8 channel currents were inhibited by the heat. Noxious cold-induced Ca2+ gates were blocked by the ACA although menthol-induced TRPM8 currents were not blocked by ACA and 2-APB. In conclusion, the
results suggested that activation of TRPM8 either by menthol or nociceptive cold can activate TRPM8 channels although we observed
the protective role of heat, ACA and 2-APB through a TRPM8 channel in nociceptive cold-activated DRG neurons. Since cold allodynia
is a common feature of neuropathic pain and diseases of sensory neuron, our findings are relevant to the etiology of neuropathology
in DRG neurons. 相似文献
42.
Sulieman Ibraheem Shelash Al-Hawary Saade Abdalkareem Jasim Ahmed Hjazi Enwa Felix Oghenemaro Irwanjot Kaur Abhinav Kumar Ahmed Muzahem Al-Ani Enas R. Alwaily Ahmed Huseen Redhee Yasser Fakri Mustafa 《Cell biochemistry and function》2024,42(2):e3978
Ovarian cancer continues to be a difficult medical issue that affects millions of individuals worldwide. Important platforms for cancer immunotherapy include checkpoint inhibitors, chimeric antigen receptor T cells, bispecific antibodies, cancer vaccines, and other cell-based treatments. To avoid numerous infectious illnesses, conventional vaccinations based on synthetic peptides, recombinant subunit vaccines, and live attenuated and inactivated pathogens are frequently utilized. Vaccine manufacturing processes, however, are not entirely safe and carry a significant danger of contaminating living microorganisms. As a result, the creation of substitute vaccinations is required for both viral and noninfectious illnesses, including cancer. Recently, there has been testing of nucleic acid vaccines, or NAVs, as a cancer therapeutic. Tumor antigens (TAs) are genetically encoded by DNA and mRNA vaccines, which the host uses to trigger immune responses against ovarian cancer cells that exhibit the TAs. Despite being straightforward, safe, and easy to produce, NAVs are not currently thought to be an ideal replacement for peptide vaccines. Some obstacles to this strategy include selecting the appropriate therapeutic agents (TAs), inadequate immunogenicity, and the immunosuppressive characteristic of ovarian cancer. We focus on strategies that have been employed to increase NAVs' effectiveness in the fight against ovarian cancer in this review. 相似文献
43.
Chitooligosaccharides are nontoxic and water-soluble compounds obtained by enzymatic degradation of chitosan, which is derived from chitin by a deacetylation process. Chitooligosaccharides possess broad range of activities such as antitumour, antifungal, antibacterial activities. Sulfated chitooligosaccharides (SCOSs) with different molecular weights were synthesized by a random sulfation reaction. In the present study, anti-HIV-1 properties of SCOSs and the impact of molecular weight on their inhibitory activity were investigated. SCOS III (MW 3-5 kDa) was found to be the most effective compound to inhibit HIV-1 replication. At nontoxic concentrations, SCOS III exhibited remarkable inhibitory activities on HIV-1-induced syncytia formation (EC50 2.19 μg/ml), lytic effect (EC50 1.43 μg/ml), and p24 antigen production (EC50 4.33 μg/ml and 7.76 μg/ml for HIV-1RF and HIV-1Ba-L, respectively). In contrast, unsulfated chitooligosaccharides showed no activity against HIV-1. Furthermore, it was found that SCOS III blocked viral entry and virus-cell fusion probably via disrupting the binding of HIV-1 gp120 to CD4 cell surface receptor. These results suggest that sulfated chitooligosaccharides represent novel candidates for the development of anti-HIV-1 agent. 相似文献
44.
Summary The antibacterial effect of negapillin in combination with other antibiotics and sulfa drugs was studied using Gram+ve and Gram-ve bacteria as test organisms. Subsequently, various proportions of negapillin in combination with the antibiotics and the sulfa drugs were examined to determine the optimum proportions which exert maximum inhibitory effects on the bacterial growth. Mixtures of these antibiotics with negapillin were found more superior not only as antimicrobial agents but also for having comparatively low toxicity. 相似文献
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48.
M Ben Saad J D Baylé 《Comptes rendus des séances de la Société de biologie et de ses filiales》1984,178(6):677-683
Annual variations in plasma testosterone and cortisol levels were determined in male wild rabbits caught monthly in their natural biotope (Zembra island). For comparison, a group of warrens was hold in semi-captivity close to Tunis. In both conditions plasma testosterone concentrations were low from January to September and peak values were observed in October. Captive warrens exhibited a clear cut annual rhythm in plasma cortisol levels also and ether stress resulted in a very large increase of cortisolemia. In rabbits caught in ther habitat however, cortisol concentrations were always extremely high throughout the year, probably due to stressing conditions. Interestingly, wild rabbits of Zembra island appear to subsist from a very remote past without any contact with other strains. As to Zembra, it is a hardly get-at-able, uninhabited island which is a part of a strictly protected natural reserve and constitutes a fairly useful and valuable ecosystem. 相似文献
49.
M L Saad P G Kovalenko V N Zaets G V Korniets Iu D Shaturski?IaPKholodova A P Galkin 《Ukrainski? biokhimicheski? zhurnal》1992,64(6):84-87
Differences in the composition and amount of proteins synthesized in the cell culture and leaves of field plants Serratula coronata have been shown. They proceed from differences in intensity of synthesis of secondary metabolites, ecdysteroids, whose content in the cell culture is considerably lower. 相似文献
50.