全文获取类型
收费全文 | 1248篇 |
免费 | 61篇 |
国内免费 | 1篇 |
出版年
2013年 | 20篇 |
2012年 | 21篇 |
2011年 | 24篇 |
2010年 | 31篇 |
2009年 | 28篇 |
2008年 | 31篇 |
2007年 | 33篇 |
2006年 | 38篇 |
2005年 | 29篇 |
2004年 | 18篇 |
1999年 | 15篇 |
1998年 | 17篇 |
1997年 | 33篇 |
1996年 | 23篇 |
1995年 | 20篇 |
1994年 | 19篇 |
1993年 | 23篇 |
1992年 | 22篇 |
1991年 | 17篇 |
1990年 | 29篇 |
1989年 | 21篇 |
1988年 | 30篇 |
1987年 | 20篇 |
1986年 | 18篇 |
1985年 | 16篇 |
1984年 | 22篇 |
1983年 | 17篇 |
1982年 | 24篇 |
1981年 | 24篇 |
1980年 | 14篇 |
1979年 | 25篇 |
1978年 | 19篇 |
1977年 | 15篇 |
1976年 | 15篇 |
1975年 | 26篇 |
1974年 | 16篇 |
1973年 | 15篇 |
1972年 | 22篇 |
1971年 | 16篇 |
1959年 | 27篇 |
1958年 | 43篇 |
1957年 | 21篇 |
1956年 | 30篇 |
1955年 | 33篇 |
1954年 | 34篇 |
1953年 | 29篇 |
1952年 | 29篇 |
1951年 | 30篇 |
1950年 | 32篇 |
1949年 | 23篇 |
排序方式: 共有1310条查询结果,搜索用时 250 毫秒
991.
SARAH I. DUNCAN SUSAN E. RIECHERT BENJAMIN M. FITZPATRICK JAMES A. FORDYCE 《Molecular ecology》2010,19(4):810-818
The evolution of sociality remains a challenge in evolutionary biology and a central question is whether association between kin is a critical factor favouring the evolution of cooperation. This study examines genetic structure of Anelosimus studiosus, a spider exhibiting polymorphic social behaviour. Two phenotypes have been identified: an ‘asocial’ phenotype with solitary female nests and a ‘social’ phenotype with multi‐female/communal nests. To address the questions of whether these phenotypes are differentiated populations and whether cooperative individuals are closely related, we used microsatellites to analyse individuals from both communal and solitary nests. We found no evidence of differentiation between social and solitary samples, implying high rates of interbreeding. This is consistent with the hypothesis that these phenotypes coexist as a behavioural polymorphism within populations. Pairwise relatedness coefficients were used to test whether cooperating individuals are more closely related than expected by chance. Pairwise relatedness of females sharing communal webs averaged 0.25, the level expected for half‐siblings and significantly more closely related than random pairs from the population. Solitary females collected at similar distances to the communal spider pairs were also more closely related than expected by chance (mean relatedness = 0.18), but less related than social pairs. These results imply that low dispersal contributes to increase likelihood of interaction between kin, but relatedness between social pairs is not explained by spatial structure alone. We propose that these phenotypes represent stages in the evolution of sociality, where viscous population structure creates opportunities for kin selection and cooperation is favoured under certain environmental conditions. 相似文献
992.
1. Estimating the probability of establishment is an important step toward managing ecological risk posed by natural or intentional introductions. Species introductions in certain geographic areas may pose less of a threat when environmental conditions are unlikely to promote long-term establishment. Species commonly referred to as 'tilapia' have become widespread in certain areas of the southeastern United States, yet concerns remain regarding their potential spread to other areas.
2. We created a model to predict the survival of tilapia in Georgia (U.S.A.) based on individual 'thermal experience'. Laboratory experiments were conducted to measure the duration of survival of two strains of tilapia, Oreochromis nilotica and O. nilotica × O. aureus hybrids, exposed to a variety of temperature regimes with three minimum temperatures and two rates of temperature decline.
3. We used Mayfield hierarchical logistic regression (MHLR) to describe the daily probability of survival as a function of the rate of temperature decline, average sustained temperature, tilapia strain and mass–length ratio.
4. Tilapia generally survived constant minimum temperatures over 12 °C but survival rate was mediated by the rate of decline, mass–length ratio and strain. For every 1 °C increase in minimum temperature, tilapia were 2.76 times more likely to survive. Fish exposed to rapid temperature decline were less likely to survive than those exposed to slowly decreasing temperature. More robust fish (higher mass–length ratio) were more likely to survive, and strain had a minimal effect despite being supported by the best MHLR model.
5. Our model can be used to estimate survival probability for tilapia under known surface water temperature regimes. Using MLHR in conjunction with experimental tolerance data may be useful for estimating the likelihood of establishment of potentially invasive species. 相似文献
2. We created a model to predict the survival of tilapia in Georgia (U.S.A.) based on individual 'thermal experience'. Laboratory experiments were conducted to measure the duration of survival of two strains of tilapia, Oreochromis nilotica and O. nilotica × O. aureus hybrids, exposed to a variety of temperature regimes with three minimum temperatures and two rates of temperature decline.
3. We used Mayfield hierarchical logistic regression (MHLR) to describe the daily probability of survival as a function of the rate of temperature decline, average sustained temperature, tilapia strain and mass–length ratio.
4. Tilapia generally survived constant minimum temperatures over 12 °C but survival rate was mediated by the rate of decline, mass–length ratio and strain. For every 1 °C increase in minimum temperature, tilapia were 2.76 times more likely to survive. Fish exposed to rapid temperature decline were less likely to survive than those exposed to slowly decreasing temperature. More robust fish (higher mass–length ratio) were more likely to survive, and strain had a minimal effect despite being supported by the best MHLR model.
5. Our model can be used to estimate survival probability for tilapia under known surface water temperature regimes. Using MLHR in conjunction with experimental tolerance data may be useful for estimating the likelihood of establishment of potentially invasive species. 相似文献
993.
HAMILTON LD BARCLAY RK WILKINS MH BROWN GL WILSON HR MARVIN DA EPHRUSSI-TAYLOR H SIMMONS NS 《The Journal of biophysical and biochemical cytology》1959,5(3):397-404
DNA's from diverse cells of different species and from diverse tissues give the same x-ray diffraction pattern. The presently observable structure of DNA appears, then, to be the same in all cells. Thus, DNA in the resting state-the stored genetic material, from sperm of Paracentrotus lividus, Arbacia lixula, and salmon and from T(2) and T(7) bacteriophage-gives a pattern indistinguishable from DNA from very rapidly dividing cells, e.g., human acute leukemic leukocytes, human leukemic myeloid cells, mouse sarcoma 180, and bacteria-E. coli and pneumococci-during their logarithmic growth. The same x-ray patterns are given by DNA's from more slowly dividing tissues, e.g. calf liver, calf thymus, and human normal and leukemic lymphatic tissue. DNA from chicken erythrocytes-a DNA presumably metabolically inert-gives a similar picture. DNA's from several sources with a wide range in nitrogen base ratios, prepared independently by different workers using various methods, have given final products in varying yield; these all gave the same x-ray pattern, suggesting that all DNA is in the double-helical configuration. Finally, separation of the DNA molecule into a number of fractions with a varying adenine + thymine:guanine + cytosine ratio, but a constant adenine:thymine and guanine:cytosine ratio, each giving the same x-ray pattern as the original whole molecule, suggests that DNA cannot exist in significant amounts in forms other than the double-helix. X-ray diffraction photographs of sperm heads, extracted nucleoprotamine, calf thymus nuclei and extracted nucleohistone, and of chicken erythrocyte nuclei, are not all as well defined as those given by extracted DNA, but it is clear from the general characteristics of the pattern that much of the DNA bound to protein in these nuclei has the usual helical configuration, and that the double-helical structure of DNA exists in the cell and is not an artifact. 相似文献
994.
995.
SUSAN J. HEDLEY DAVID J. GAWKRODGER ANTHONY P WEETMAN SHEILA MACNEIL 《Pigment cell & melanoma research》1998,11(1):45-56
Normal human skin melanocytes do not pigment consistently to α-melanocyte stimulating hormone (α-MSH) in culture. The aim of this study was to establish media conditions in which to obtain a reproducible melanogenic response to α-MSH in these cells. Twenty-five media of varying mitogen composition were examined. As previously noted by other workers, melanocyte morphology and proliferation are greatly affected by media composition. However, under the majority of media conditions that supported melanocyte survival and proliferation, cells did not respond to α-MSH with any consistent increase in dopa oxidase activity or melanin content. In only one medium condition, where basic fibroblast growth factor (bFGF) was the sole mitogen present, α-MSH induced both an increase in dopa oxidase activity (at 48%) and in melanin content (of 283%). 相似文献
996.
997.
998.
The mermithid parasite, Isomermis lairdi Mondet, Poinar & Bernadou (Nematoda: Mermithidae), is known to have a major impact on populations of Simulium damnosum s.l . Theobald (Diptera: Simuliidae) and on their efficiency as vectors of Onchocerca volvulus (Leuckart) (Nematoda: Filarioidea). However, the value of I. lairdi and other mermithid parasites as potential means of integrated vector control has not been fully realized. This is partly because traditional taxonomic approaches have been insufficient for describing and analysing important aspects of their biology and host range. In total, rDNA barcode sequences have been obtained from over 70 I. lairdi mermithids found parasitizing S. damnosum s.l . larvae in three different rivers. No two sequences were found to vary by more than 0.5%, and cytospecies identification of mermithid hosts revealed that I. lairdi with identical rDNA barcodes can parasitize multiple cytoforms of the S. damnosum complex, including S. squamosum (Enderlein). Phylogenetic analysis using a partial sequence from the 18S ribosomal DNA barcode, grouped I. lairdi in a monophyletic group with Gastromermis viridis Welch (Nematoda: Mermithidae) and Isomermis wisconsinensis Welch (Nematoda: Mermithidae). 相似文献
999.
Primates tend to prefer specific plant foods, and primate home ranges may contain only a subset of food species present in an area. Thus, primate feeding strategies should be sensitive to the phenology of specific species encountered within the home range in addition to responding to larger scale phenomena such as seasonal changes in rainfall or temperature. We studied three groups of Javan gibbons (Hylobates moloch) in the Gunung Halimun‐Salak National Park, Indonesia from April 2008 to March 2009 and used general linear mixed models (GLMM) and a model selection procedure to investigate the effects of variation in fruit and flower availability on gibbon behavior. Preferred foods were defined as foods that are overselected relative to their abundance, while important food species were those that comprised >5% of feeding time. All important species were also preferred. Season and measurements of flower and fruit availability affected fruit‐feeding time, daily path lengths (DPL), and dietary breadth. Models that included the availability of preferred foods as independent variables generally showed better explanatory power than models that used overall fruit or flower availability. For one group, fruit and preferred fruit abundance had the strongest effects on diets and DPL in the models selected, while another group was more responsive to changes in flower availability. Temporal variation in plant part consumption was not correlated in neighboring groups. Our results suggest that fine‐scale local factors are important determinants of gibbon foraging strategies. Am. J. Primatol. 74:1154‐1167, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
1000.
The neural crest of vertebrate embryos has been used to elucidate steps involved in early embryonic cellular processes such as differentiation and migration. Neural crest cells form a ridge along the dorsal midline and subsequently they migrate throughout the embryo and differentiate into a wide variety of cell types. Intrinsic factors and environmental cues distributed along the neural tube, along the migratory pathways, and/or at the location of arrest influence the fate of neural crest cells. Although premigratory cells of the cranial and trunk neural crest exhibit differences in their differentiation potentials, premigratory trunk neural crest cells are generally assumed to have equivalent developmental potentials. Axolotl neural crest cells from different regions of origin, different stages of development, and challenged with different culture media have been analyzed for differentiation preferences pertaining to the pigment cell lineages. We report region-dependent differentiation of chromatophores from trunk neural crest at two developmental stages. Also, dosage with guanosine produces region-specific influences on the production of xanthophores from wild-type embryos. Our results support the hypothesis that spatial and temporal differences among premigratory trunk neural crest cells found along the anteroposterior axis influence developmental potentials and diminish the equivalency of axolotl neural crest cells. 相似文献