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41.
棉铃虫致病菌BT—931菌株的应用研究 总被引:1,自引:0,他引:1
1992-1993年,作者从菏泽、聊城棉田采集的自然罹病死亡棉铃虫幼虫体内,分离到2个较高毒效的Bt菌株,编号为BT—931和BT—021,经室内毒力测定和田间药效试验表明,防效及保蕾效果接近或超过化学农药,菌药混剂3—5天平均防治效果达87.0-91.6%,增效作用显著。经1994年大田防治示范表明,利用BT—931菌剂。配合化学农药防治棉铃虫,具有成本低、保护天敌、持效期长、增产效益高等优点。本研究对菌剂的田间应用技术进行了试验示范。 相似文献
42.
Soo Wan Nam Byung Moon Kim Bong Hyun Chung Dae Ook Kang Jong Seog Ahn 《Biotechnology letters》1994,16(9):897-902
Summary For the secretion of human lipocortin-1 (LC-1) in yeast, a expression and secretion vector was constructed by using the promoter and signal sequence of glucoamylase gene (STA1) of Saccharomyces diastaticus. After the cDNA of human LC-1 was ligated with the secretion vector, the resulting hybrid plasmid was transformed into S. diastaticus. When the recombinant S. diastaticus was cultivated in YPD medium, LC-1 was expressed and secreted into the extracellular medium, yielding LC-1 protein at a concentration of 2.5 g/mL. 相似文献
43.
本项研究将野外捕获的成年雄性高原鼠兔(Ochotonacurzoniae)分3组进行不同光制的饲养,以检验光周期对其神经内分泌─—生殖轴功能的影响:(1)长光照组睾丸及附属性腺的重量和血浆睾酮水平均明显高于自然光组(p<0.05)和短光照组(p<0.01)。(2)长光照组的松果腺重量(1.5±0.1毫克,n=15)和褪黑激素含量(89.7±5.8微微克/松果腺),均显著低于其它两组。(3)自然光组和短光照组性腺的显著萌发表明,在高原鼠兔季节性繁殖的年周期中,其神经内分泌─—生殖轴对春分前短光照的抑制作用具有不应期。 相似文献
44.
艾氏剂环氧化酶及细胞色素P-450对小菜蛾抗药性发展的影响 总被引:1,自引:0,他引:1
本文对室内长期饲养的小菜蛾(Plutella xylostella L.)敏感品系和田间采集的抗性种群体内的艾氏剂平氧化酶及细胞色素P-450进行了比较研究。结果证明,艾氏剂环氧化酶在感性和抗性小菜蛾间存在着量及质的差异。抗性种群的艾氏剂环氧化酶的Vmax和Km值分别为感性品系的5.4%和6.5倍。抗性种群的细胞色素P-450的含量是感性品系的1.1—1.3倍。艾氏剂环氧化酶在量上及质上的差异及细胞色素P-450含量的提高是导致小菜蛾抗药性发生与发展的重要机制之一。而且质的差异较之量的差异可能起着更为重要的作用. 相似文献
45.
46.
H. M. Rüffer Liwei Wan A. Lübbert K. Schügerl 《Bioprocess and biosystems engineering》1994,11(4):153-159
Gas-residence time distribution (RTD) response curves measured in a 23 m high pilot plant airlift tower loop reactor, which consisted of a riser, a special downcomer construction and at the top of the riser a large head. The measurements were evaluated by means of a deterministic dispersion model, which yielded the following particular parameters for the riser, downcomer and the head: Gas-Bo numbers, gas-mean residence times, gas holdups, liquid velocities, gas and liquid circulation times as well as a fraction of the large and small bubbles in a model medium (water) and during cultivation of baker's yeast.List of Symbols
A
cross section
-
Bo
Bodenstein number
-
Bo
d
(= l
d
w
G,d
/D
d
)
-
Bo
h
(= l
h
w
G,h
/D
h
)
-
Bo
r
(= l
r
w
G,r
/D
r
)
-
D
longitudinal dispersion coefficient
-
E
gas holdup
-
E(t)
RTD-density function
-
L, l
length parameter
-
q
fraction of the gas throughput which is not recirculated (approximately equal to fraction of the large bubbles)
-
r
fraction of the throughput which is recirculated (approximately equal to the fraction of the small bubbles)
-
t
c
circulation time
-
t
cL
liquid circulation time
-
t
c,L
*
liquid circulation time calculated from the measured w
Ld
in the downcomer
-
V
h
hydrodynamical calculated gas-liquid volume
-
V
d
h
(=V
d+0.75/2 V
k
)
-
V
k
h
=(0.25V
k
)
-
V
r
h
= (V
r+0.75/2 V
k
)
-
V
L
liquid volume
-
V
G
dispersed gas volume
-
V
G
*
gas throughput at the gas distributor (given in m3/h) under standard conditions, 1 bar and 25°C)
-
V
G,d
*
gas throughput in downcomer (=V
G
*
)
-
V
G,h
*
gas throughput in head (=V
G
*
)
-
V
G,r
*
gas throughput in riser (V
G
*
(1+)
-
w
g
gas velocity
-
w
G,rel
relative gas velocity with respect to the liquid velocity w
L
-
w
G,d
gas velocity in the downcomer (=w
G,rel
–w
Ld
)
-
w
G,h
gas velocity in the head (=w
G,rel
) (since wLh = o)
-
w
G,r
gas velocity in the riser (=w
G,rel
+w
Lr
)
-
w
L
liquid velocity
-
w
L,d
liquid velocity in the downcomer measured with mass flow meter
-
w
sg
·w
SL
superficial gas and liquid velocities
-
first moment of the response curve
-
mean residence time
Indices
d
downcomer
-
G
gas phase
-
h
head
-
L
liquid phase
-
r
riser
-
h
hydrodynamic (upper position)
Dedicated to the 65th birthday of Proffessor Fritz Wagner.The authors gratefully acknowledge the financial support by the Krupp Industrietechnik, Grevenbroich and the support of Pleser Co, Darmstadt. H. M. Rüffer thanks the Verband der Chemischen Industrie for a Fond der Chemie scholarship, and W. Liwei thanks the government of Lower Saxony for a graduate scholarship. 相似文献
47.
The DNaseI hypersensitive site 2 (HS2) of human β-globin locus control region(LCR) is required for the high level expression of human β-globin genes.In the present study,a stage-specific protein factor (LPF-β) was identified in the nuclear extract prepared from mouse fetal liver at d 18 of gestation,which could bind to the HS2 region of human β-globin LCR.We also found that the shift band of LPF-β factor could be competed by human β-globin promoter.However,it couldn‘t be competed by human ε-globin promoter or by human ^Aγ-globin promoter.Furthermore,our data demonstrated that the binding-sequence of LPF-β factor is 5‘CACACCCTA 3‘,which is located at the HS2 region of β-LCR(from-10845 to-10853 bp)and human β-globin promoter(from-92 to -84 bp).We speculated that these regions containing the CACCC box in both the human β-globin promoter and HS2 might function as stage selector elements in the regulation of human β-globin switching and the LPF-β factor might be a stage-specific protein factor involved in the regulation of human β-globin gene expression. 相似文献
48.
D. P. Chora L. Reddy S. K. Gupta L. Wan P. A. Mathieu R. L. Shoemaker J. S. Rhim 《In vitro cellular & developmental biology. Animal》1994,30(8):539-546
Summary Cystic fibrosis (CF) involves abnormalities in mucus production and secretion of the airway. Studies of the regulation of
airway mucin production and secretion has been difficult due to the lack of in vitro models of the airway epithelial cells
which express functional differentiation. Because the majority of the mucin in the airway is apparently produced by the submucosal
glands, we have focused our attention on the development of cell culture models of human airway submucosal glands. This report
describes the propagation of CF airway submucosal gland epithelial cells which continue to express mucin production. The CF
bronchus was obtained from a 31-yr-old patient who received a double lung transplant. The glands were dissected out and primary
cultures prepared by the explant/outgrowth procedure. The cells were immortalized by infection with Adl2-SV40 hybrid virus.
The cultures are maintained in serum-free keratinocyte basal medium supplemented with insulin (5μg/ml), hydrocortisone (0.5μg/ml), epidermal growth factor (10 ng/ml), bovine pituitary extract (25μg/ml), and antibiotics. Cultures were passaged using 0.125% trypsin in Ca+2 and Mg+2-free Hanks’, balanced salt solution. Polymerase chain reaction (PCR) analysis demonstrated that the cells were homozygous
for the ΔF508 mutation. Morphologic observations showed that the cells were epithelial and were interconnected by sparsely
distributed desmosomes. Their cytoplasm contained secretory-type structures including abundant Golgi, rough endoplasmic reticulum,
and secretory vesicles. Immunofluorescent studies determined that all cells were positive for cytokeratins, mucin glycoconjugates,
and cystic fibrosis transmembrane conductance regulator. The cultures secreted substantial amounts of mucin glycoproteins
and expressed the MUC-2 mucin gene. Patch clamp experiments revealed that the cells expressed defective Cl− channels which were not activated by Forskolin. 相似文献
49.
Ar^+,远红外激光,γ射线单一及复合处理水稻的诱变效果 总被引:1,自引:0,他引:1
用Ar~+、远红外两种激光和~(60)Co—r射线单一或复合处理两个籼稻品种的干种子,分析和比较了不同处理对水稻的当代生物学效应及处理二代的变异频率。结果表明,两种激光对当代的几个性状均表现为刺激效应,并有减轻,射线辐射损伤的作用,复合处理二代的变异频率和变异类型数明显高于相应的单一处理。说明复合处理是提高激光育种效果的有效方法。 相似文献
50.
Wan Noraini Wan Yusof Menaka Nagaratnam Chong-lek Koh Savithri Puthucheary Tikki Pang 《Microbiology and immunology》1993,37(8):667-670
Human mononuclear cells pre-labeled with [3H]arachidonic acid were shown to release metabolites following in vitro addition of heat-killed Salmonella typhi (HKST). The amount of label released was significantly higher than that seen with live S. typhi (LST). Addition of increasing amounts of HKST resulted in an increased release of metabolites. Enzyme immunoassay of the culture supernatants revealed that the bulk of the metabolite released was prostaglandin E2 (PGE2). Leukotriene B4 (LTB4) and leukotriene C4 (LTC4) were not detectable in the culture supernatants. The significance and implications of these results are discussed. 相似文献