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91.
Sensitivity to metalaxyl of Phytophthora infestans populations in potato crops in south-west England in 1980 and 1981 总被引:5,自引:0,他引:5
A laboratory test was developed to assess the sensitivity of field populations of Phytophthora infestans to metalaxyl. Discs of potato leaf tissue were floated upon solutions of the fungicide at different concentrations and inoculated with spores. The extent of symptom development was noted after incubation under standard conditions for 5–6 days. In preliminary experiments growth of isolates of P. infestans obtained from culture collections was severely inhibited in discs treated at 2 μg/ml. By contrast the development of an isolate obtained from a crop in Eire in which blight control with metalaxyl had failed, and known to be markedly less sensitive in vitro, was unaffected in discs treated at 100 μg/ml. During the summer of 1980, 234 samples of P. infestans were obtained from 20 sites in south-west England, 10 of which had received sprays containing metalaxyl and 10 of which had not. All samples were sensitive to metalaxyl applied at 2 μg/ml. In 1981, 35 sites within the same area, 30 of which had received sprays containing either metalaxyl or ofurace (a related fungicide), were similarly surveyed. Most of the 79 samples of P. infestans examined proved sensitive and at all sites the amount of blight was small. However, at three sites, including one not treated with acylalanine fungicides, strains were found which were unaffected by 100 μg/ml metalaxyl in leaf disc tests. These findings are discussed in relation to the development of resistant blight in other areas and to the use of fungicide mixtures. 相似文献
92.
The carbon canopy economy of the association between cowpea and the parasitic angiosperm Striga gesnerioides 总被引:2,自引:2,他引:0
The association between the parasite Striga gesnerioides and cowpea (Vigna unguiculata) was investigated using measurements of growth and gas exchange together with calculations of the carbon budget of the association. Striga gesnerioides has a very low photosynthetic capacity coupled with high rates of respiration. Even at photosynthetic light saturation shoots exhibit no net carbon gain. Thus S. gesnerioides is highly dependent on its host for carbon as well as for water and inorganic solutes. It is estimated that 70% of the carbon transferred from host to parasite is used in parasite respiration. Infected cowpea had a lower photosynthetic capacity, at times less than half that of uninfected plants. Infection with S. gesnerioides reduced the growth of cowpea by 75%. Calculations indicate that the loss of carbon from the host by export to the parasite is more important than reduced photosynthetic capacity of the host in accounting for the observed growth reductions. 相似文献
93.
Bayesian analyses for a multiple capture-recapture model 总被引:3,自引:0,他引:3
94.
Salt-Induced Ultrastructural Damage to Mitochondria in Root Tips of a Salt-Sensitive Ecotype of Agrostis Stolonifera 总被引:4,自引:0,他引:4
SMITH M. M.; HODSON M. J.; OPIK HELGI; WAINWRIGHT S. J. 《Journal of experimental botany》1982,33(5):886-895
In root tips of a salt-sensitive ecotype of Agrostis stolonifera,treatment with 100 mmol I1 NaCl for two weeks resultedin conspicuous structural damage to mitochondria, which becamedeficient in cristae, swelled and vacuolated. A salt-tolerantecotype maintained normal root tip mitochondrial structure underthe same salt treatment, in spite of accumulating higher levelsof sodium and chloride in its roots. The subcellular distributionof chloride, as judged by electron microscopy after precipitationwith silver ions, was identical in both ecotypes. 相似文献
95.
96.
The vacuolar pH (pHv) and the cytoplasmic pH (pHc) of the marinegiant-celled green alga Chaetomorpha darwinii were measuredby pH microelectrode techniques on extracted vacuolar sap, andby the [I4C]DMO distribution method respectively. Equilibrationof DMO occurred with a half-time of about 2 h, with an apparentPDMO of 3.6 x 105 cm s1, but the vacuolar concentrationof free, undissociated DMO was always less than the externalconcentration. The explanation offered for freshwater giant-celledalgae of net DMO leakage across the plasmalemma cannotapply to Chaetomorpha darwinii, since electrically-driven DMOexit from the cytoplasm should be similar across the plasmalemmaand the tonoplast in these cells with large, vacuole-positivepotential differences across the tonoplast. pHc was accordinglycomputed assuming either tonoplast or plasmalemma equilibrationof DMO, with correction for DMO metabolism within the cell.pHc was 8.08.3 in the light in artificial seawater (pHoabout 8.0), was some 0.5 units lower in the dark, and was slightlylower with an external pH of 7. Vacuolar pH was 6.56.9,without consistent effects of illumination or of external pHof 7 rather than 8. While µH+ at the tonoplast was similarto that in giant-celled freshwater algae (although with a greatercontribution from relative to pH), µH+ at the plasmalemmawas less than 8 kJ mol1, i.e. less than one-third ofthe value in freshwater green algae. µNa+ was some 13kJ mol1 at the plasmalemma. The possibility that theprimary active transport process at the plasmalemma of Chaetomorphadarwinii (and certain other marine algae) is Na+ efflux ratherthan H+ efflux is discussed. 相似文献
97.
98.
Influence of crop background on aphids and other phytophagous insects on Brussels sprouts 总被引:1,自引:0,他引:1
JUDITH G. SMITH 《The Annals of applied biology》1976,83(1):1-13
More Brevicoryne brassicae and other alate aphids were caught in yellow water-traps in a weed-free crop of Brussels sprouts than in a crop with a weedy background. More B. brassicae colonized Brussels sprout plants in bare soil than in weeds; very few alatae were attracted to cruciferous weeds in the crop. Results in 1 yr suggest that initially larger populations on the weed-free sprouts became smaller than populations on the weedy sprouts because the larger aphid population attracted more natural enemies. Aleyrodes brassicae and certain Lepidoptera were also more abundant on sprout plants in bare soil than on sprouts surrounded by weeds; more adult A. brassicae were caught in water traps over the bare soil. More A. brassicae were present on sprout plants surrounded by a green than by a brown cloth background but the differences were not significant (P < 0–05). Numbers of B. brassicae on sprout plants with green and brown backgrounds varied greatly and did not differ significantly. In field cages, B. brassicae alatae were more attracted to potted sprout plants surrounded by bare soil than to ones surrounded by rings of living or cut grass or by artificial green rings. This effect was greater with small than with large sprout plants surrounded by grass rings. The maintenance of a limited weed cover is considered potentially useful in integrated control of some brassica pests. 相似文献
99.
J. R. COOK SANDRA SMITH HAGGARD PATRICIA HARRIS 《The Journal of eukaryotic microbiology》1976,23(3):368-373
SYNOPSIS. In populations of Euglena gracilis strain Z synchronized by cultivation on a repetitive light-dark cycle, chloroplasts undergo cyclic changes in structure. During most of the light period chloroplasts are relatively compact with closely appressed lamellae; during the dark (division) period the chloroplasts become quite distended. This change persists for at least one cycle even when the cells are left in continuous light, suggesting that the periodicity may be related more to the age of the cell than to a direct effect of light. In addition, the pyrenoid in synchronized cells has a transient existence, being present only in the first half of the light period. 相似文献
100.
JOSEPH J. NAPOLITANO BARBARA-HELENE SMITH 《The Journal of eukaryotic microbiology》1975,22(2):196-199
Nuclear division in synchronized cultures of the ameboflagellate Adelphamoeba galeacystis has been described. Division in this organism is typically promitotic. It occurs within an intact nuclear membrane and is characterized by the persistence of the nucleolus and its transformation into 2 polar masses. The nucleolus is stained with pyronin-Y by the methyl green pyronin-Y technic, and with Heidenhain's hematoxylin, but is unstained by the Feulgen reaction. The reaction with these stains is removed after digestion of the nucleolus by ribonuclease. During mitosis the nucleolus undergoes an orderly series of vacuolizations before forming the polar masses. The chromatin is Feulgen positive, stains with methyl green by the methyl green pyronin-Y technic and undergoes a series of characteristic changes during the division process. Synchronization of amebae grown on coverglasses was accomplished by transfer of cells from 30 to 38.5 C for a period of 100 min. A temporal sequence of nucleolar and chromatin participation in the nuclear division of this organism is suggested. 相似文献