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J M Schlumpberger I L Weissman V L Scofield 《The Journal of experimental zoology》1984,229(3):401-411
Blood cells of the colonial tunicate Botryllus were separated by density gradient centrifugation in Percoll. Unseparated blood cells were used to immunize mice for development of hybridoma cell lines producing anti-Botryllus monoclonal antibodies. These antibodies identify specific subpopulations of blood cells, indicating possible divisions of these cells into defined subgroups sharing particular differentiation antigens. Additional studies utilizing fluorescein- conjugated lectins also revealed differential binding to density- and monoclonal antibody-defined blood cell fractions. These methods allow separation of the different Botryllus blood cell types for functional studies. 相似文献
696.
Effect of respiratory apparatus on respiration 总被引:1,自引:0,他引:1
Weissman C.; Askanazi J.; Milic-Emili J.; Kinney J. M. 《Journal of applied physiology》1984,57(2):475-480
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Drew Weissman Mohamad-Gabriel Alameh Thushan de Silva Paul Collini Hailey Hornsby Rebecca Brown Celia C. LaBranche Robert J. Edwards Laura Sutherland Sampa Santra Katayoun Mansouri Sophie Gobeil Charlene McDanal Norbert Pardi Nick Hengartner Paulo J.C. Lin Ying Tam Pamela A. Shaw David C. Montefiori 《Cell host & microbe》2021,29(1):23-31.e4
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Self-renewal, differentiation or death: regulation and manipulation of hematopoietic stem cell fate. 总被引:10,自引:0,他引:10
Hematopoietic stem cells (HSCs) are the rare cells from which all hematopoietic cells are derived. The absence of HSCs is not compatible with life because many essential cells, such as myeloid and erythroid cells, are short lived. The hematopoietic system is the first essential organ system that fails following cytotoxic treatments. It is the vulnerability of HSCs that prevents regeneration following treatment and thus long-term survival. Because HSCs have the capacity to regenerate a functional hematopoietic system, the manipulation of these cells in vitro holds many promises for gene-therapeutic and other applications; however, these are severely curtailed by current difficulties in maintaining and expanding HSCs in culture. This review focuses on recent approaches towards understanding how the HSC compartment is regulated in vivo and discusses how this knowledge might be applied to manipulating HSC numbers. 相似文献
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Cytodifferentiation during spermiogenesis in Hydra littoralis was studied at the fine structural level. Concentration of nuclear material as well as specific orientation of granular and filamentous nuclear elements are apparent in two regions of the early spermatid: where the nuclear envelope is in contact with mitochondrial membranes at one pole of the cell and at an opposite region where the nucleus is closely apposed to the plasma membrane. Ultimately the mass of condensed nuclear material becomes concentrated at the mitochondrial pole of the cell. Additional electron-dense material is extruded from the nucleus into a large vacuole which is in continuity with the nuclear membrane as well as associated with Golgi lamellae and vesicles. Eventually all residual cytoplasm is sloughed, leaving the nucleus, mitochondria, and flagellum. These observations are suggestive of nucleocytoplasmic interactions during development, especially influences of mitochondria and plasma membranes on chromatin condensation. 相似文献