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81.
Patrick D. Brandt Susi Sturzenegger Varvayanis Tracey Baas Amanda F. Bolgioni Janet Alder Kimberly A. Petrie Isabel Dominguez Abigail M. Brown C. Abigail Stayart Harinder Singh Audra Van Wart Christine S. Chow Ambika Mathur Barbara M. Schreiber David A. Fruman Brent Bowden Christopher A. Wiesen Yvonne M. Golightly Chris E. Holmquist Daniel Arneman Joshua D. Hall Linda E. Hyman Kathleen L. Gould Roger Chalkley Patrick J. Brennwald Rebekah L. Layton 《PLoS biology》2021,19(7)
82.
Archana Mathur Ajay Kumar Mathur Anita Gangwar Sharawan Yadav Priyanka Verma Rajender Singh Sangwan 《In vitro cellular & developmental biology. Plant》2010,46(1):13-21
A root-derived callus line of Panax sikkimensis that stably accumulates anthocyanins was established by small cell aggregate selection method. The selected line showed a
growth index of 221.36 and an anthocyanin content of 2.76 mg/g fw (7.076% dw) in 50–60 d of growth on a modified MS medium
containing 4.5 μM 2,4-dichlorophenoxy acetic acid and 1.2 μM kinetin under 16-h light and 8-h dark photoperiodic conditions.
Incubation under continuous light increased the growth index to 435.57 but led to a marginal dilution of anthocyanin content
to 2.192 mg/g fw (6.928% dw). The purple-red pigment had absorption maximum at 528 nm. The selected callus line has shown
sustained growth and productivity for more than 6 yr now. Interestingly, pigment accumulation in the selected line did not
hinder the ginsenoside production in the callus tissue (0.9–1.2% fw). 相似文献
83.
84.
Jagesh K. Tiwari Poonam D. Sarkar SK. Pandey Jai Gopal S. Raj Kumar 《Plant Cell, Tissue and Organ Culture》2010,103(2):175-187
Interspecific potato somatic hybrids between Solanum tuberosum L. (di)haploid C-13 and 1 endosperm balance number non-tuberous wild species S. etuberosum Lindl. were produced by protoplasts electrofusion. The objective was to transfer virus resistance from this wild species
into the cultivated potatoes. Post-fusion products were cultured in VKM medium followed by regeneration of calli in MS13 K medium at 20°C under a 16-h photoperiod, and regenerants were multiplied on MS medium. Twenty-one somatic hybrids were
confirmed by RAPD, SSR and cytoplasm (chloroplast/mitochondria) type analysis possessing species-specific diagnostic bands
of corresponding parents. Tetraploid nature of these somatic hybrids was determined through flow cytometry analysis. Somatic
hybrids showed intermediate phenotypes (plant, leaves and floral morphology) to their parents in glass-house grown plants.
All the somatic hybrids were male-fertile. ELISA assay of somatic hybrids after artificial inoculation of Potato virus Y (PVY)
infection reveals high PVY resistance. 相似文献
85.
Archana Mathur Anita Gangwar Ajay K. Mathur Priyanka Verma Girish C. Uniyal Raj K. Lal 《Biotechnology letters》2010,32(3):457-461
A thin, profusely branched, fast growing hairy root line of Panax quinquefolium (American ginseng) was established by co-culturing epicotyl explants with a wild type strain of Agrobacterium rhizogenes. The transformed roots grew by over 10-fold from the initial inoculum within 8 weeks. The crude ginsenosides content in the roots was about 0.2 g/g dry wt level up to the 10th week of culture. Ginsenosides Rb2, Rd, Re, Rf and Rg1 constituted 47–49% of the crude saponin fraction between 6 and 8 weeks of growth whereas, Rc ginsenoside was accumulated only after 9th weeks when the biomass started receding. PCR amplification analysis of the hairy roots confirmed their transgenic nature by showing the presence of Ri-TL DNA with rolA, rolB and rolC genes in their genome. 相似文献
86.
Anjana Jajoo Sonal Mathur Pooja Mehta Miho Yoshoika Suleyman I. Allakhverdiev Yasusi Yamamoto 《Journal of bioenergetics and biomembranes》2010,42(1):47-53
Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating
effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient
and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of
different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion
by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS
II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted
PS II membranes. 相似文献
87.
88.
Mathur J Radhamony R Sinclair AM Donoso A Dunn N Roach E Radford D Mohaghegh PS Logan DC Kokolic K Mathur N 《Plant physiology》2010,154(4):1573-1587
Photoconvertible fluorescent proteins (FPs) are recent additions to the biologists' toolbox for understanding the living cell. Like green fluorescent protein (GFP), monomeric EosFP is bright green in color but is efficiently photoconverted into a red fluorescent form using a mild violet-blue excitation. Here, we report mEosFP-based probes that localize to the cytosol, plasma membrane invaginations, endosomes, prevacuolar vesicles, vacuoles, the endoplasmic reticulum, Golgi bodies, mitochondria, peroxisomes, and the two major cytoskeletal elements, filamentous actin and cortical microtubules. The mEosFP fusion proteins are smaller than GFP/red fluorescent protein-based probes and, as demonstrated here, provide several significant advantages for imaging of living plant cells. These include an ability to differentially color label a single cell or a group of cells in a developing organ, selectively highlight a region of a cell or a subpopulation of organelles and vesicles within a cell for tracking them, and understanding spatiotemporal aspects of interactions between similar as well as different organelles. In addition, mEosFP probes introduce a milder alternative to fluorescence recovery after photobleaching, whereby instead of photobleaching, photoconversion followed by recovery of green fluorescence can be used for estimating subcellular dynamics. Most importantly, the two fluorescent forms of mEosFP furnish bright internal controls during imaging experiments and are fully compatible with cyan fluorescent protein, GFP, yellow fluorescent protein, and red fluorescent protein fluorochromes for use in simultaneous, multicolor labeling schemes. Photoconvertible mEosFP-based subcellular probes promise to usher in a much higher degree of precision to live imaging of plant cells than has been possible so far using single-colored FPs. 相似文献
89.
Mathur N Singh J Bohra S Bohra A Vyas A 《International journal of phytoremediation》2010,12(8):798-804
An experiment was conducted to study the potential of chromium (Cr) phytoaccumulatory capabilities of four tree species viz., Anogeissus latifolia, Terminalia arjuna, Tecomella undulata, and Salvadora persica Possibility of enhancement of Cr uptake by citric acid and vesicular arbuscular mycorrhizal fungi (VAM) amendments were also tried. Cr is a major pollutant of the environment. Chromium can exist in oxidation states from III to VI, but the most stable and common forms of Cr are trivalent and hexavalent species. Cr(VI) was more toxic to the tree growth in terms of collar diameter (CD) increment in all the tree species than Cr(lll). Roots accumulated more Cr than shoots in all the tree species. There was more than 10 fold increase in root Cr content in comparison with shoot Cr content in all the trees at all the concentration of Cr and all sources of Cr. Citric acid significantly increased the Cr content in the tissues of roots in all the species under both speciation of Cr. The highest increase in Cr content brought by 20 mM citric acid addition was in A. latifolia Results suggest that Anogeissus latifolia is a potential Cr accumulator with citric acid as soil amendment. 相似文献
90.
Cell suspension cultures of Commiphora wightii, grown in modified MS medium containing 2,4-dichlorophenoxyacetic acid (0.5 mg l−1) and kinetin (0.25 mg l−1), produced ∼5 μg guggulsterone g−1 dry wt. In a 2 l stirred tank bioreactor, the biomass was 5.5 g l−1 and total guggulsterone was 36 μg l−1. 相似文献