全文获取类型
收费全文 | 2448篇 |
免费 | 338篇 |
国内免费 | 3篇 |
出版年
2021年 | 36篇 |
2020年 | 27篇 |
2019年 | 32篇 |
2018年 | 32篇 |
2017年 | 30篇 |
2016年 | 43篇 |
2015年 | 77篇 |
2014年 | 106篇 |
2013年 | 121篇 |
2012年 | 123篇 |
2011年 | 126篇 |
2010年 | 95篇 |
2009年 | 95篇 |
2008年 | 106篇 |
2007年 | 120篇 |
2006年 | 107篇 |
2005年 | 127篇 |
2004年 | 98篇 |
2003年 | 98篇 |
2002年 | 74篇 |
2001年 | 80篇 |
2000年 | 81篇 |
1999年 | 70篇 |
1998年 | 41篇 |
1997年 | 21篇 |
1996年 | 28篇 |
1995年 | 28篇 |
1994年 | 18篇 |
1993年 | 21篇 |
1992年 | 49篇 |
1991年 | 47篇 |
1990年 | 36篇 |
1989年 | 34篇 |
1988年 | 36篇 |
1987年 | 38篇 |
1986年 | 28篇 |
1985年 | 44篇 |
1984年 | 27篇 |
1983年 | 26篇 |
1982年 | 32篇 |
1981年 | 25篇 |
1979年 | 25篇 |
1978年 | 21篇 |
1977年 | 20篇 |
1975年 | 18篇 |
1974年 | 17篇 |
1973年 | 22篇 |
1971年 | 18篇 |
1970年 | 20篇 |
1968年 | 21篇 |
排序方式: 共有2789条查询结果,搜索用时 203 毫秒
251.
A novel class of 3-phenyl-2-styryl-3H-quinazolin-4-one Hsp90 inhibitors with in vitro anti-tumor activity are identified by structure-based virtual screening of a chemical database with docking simulations in the N-terminal ATP-binding site, in vitro ATPase assay using yeast Hsp90, and cell-based Her2 degradation assay in a consecutive fashion. These results exemplify the usefulness of the structure-based virtual screening with molecular docking in drug discovery. The structural features responsible for a tight binding of the inhibitors in the active site of Hsp90 are discussed in detail. 相似文献
252.
253.
Kang MJ Lee MH Shim JK Seo ST Shrestha R Cho MS Hahn JH Park DS 《Journal of microbiology and biotechnology》2007,17(11):1765-1771
A polymerase chain reaction (PCR)-based method was developed to detect the DNA of Ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. One pair of primers (RALSF and RALSR), designed using cytochrome c1 signal peptide sequences specific to R. solanacearum, produced a PCR product of 932 bp from 13 isolates of R. solanacearum from several countries. The primer specificity was then tested using DNA from 21 isolates of Ralstonia, Pseudomonas, Burkholderia, Xanthomonas, and Fusarium oxysporum f. sp. dianthi. The specificity of the cytochrome c1 signal peptide sequences in R. solanacearum was further confirmed by a DNA-dot blot analysis. Moreover, the primer pair was able to detect the pathogen in artificially inoculated soil and tomato plants. Therefore, the present results indicate that the primer pair can be effectively used for the detection of R. solanacearum in soil and host plants. 相似文献
254.
Conditions were evaluated for optimum cryopreservation of primary chicken embryo kidney (CEK) cells. The recovery of viable
CEK cells was best (50.8% viability) when the concentration of dimethyl sulfoxide (DMSO) in the freezing medium was 20% (v/v).
The viability of primary CEK cells was not influenced by the concentration of calf serum in the freezing medium, the duration
of storage at −70°C before storage in liquid nitrogen, cell concentration, or the method of addition or dilution of DMSO.
Thawed cells recovered and grew in complete growth medium similarly to cells freshly isolated from kidney, and influenza viruses
produced plaques in the monolayer. The cryopreservation procedures described here may facilitate maintenance of a standard
stock of primary CEK cells for laboratories where preparation of primary CEK cells is not an option. 相似文献
255.
Zhang H Nimmer PM Tahir SK Chen J Fryer RM Hahn KR Iciek LA Morgan SJ Nasarre MC Nelson R Preusser LC Reinhart GA Smith ML Rosenberg SH Elmore SW Tse C 《Cell death and differentiation》2007,14(5):943-951
Platelets are relatively short-lived, anucleated cells that are essential for proper hemostasis. The regulation of platelet survival in the circulation remains poorly understood. The process of platelet activation and senescence in vivo is associated with processes similar to those observed during apoptosis in nucleated cells, including loss of mitochondrial membrane potential, caspase activation, phosphatidylserine (PS) externalization, and cell shrinkage. ABT-737, a potent antagonist of Bcl-2, Bcl-X(L), and Bcl-w, induces apoptosis in nucleated cells dependent on these proteins for survival. In vivo, ABT-737 induces a reduction of circulating platelets that is maintained during drug therapy, followed by recovery to normal levels within several days after treatment cessation. Whole body scintography utilizing ([111])Indium-labeled platelets in dogs shows that ABT-737-induced platelet clearance is primarily mediated by the liver. In vitro, ABT-737 treatment leads to activation of key apoptotic processes including cytochrome c release, caspase-3 activation, and PS externalization in isolated platelets. Despite these changes, ABT-737 is ineffective in promoting platelet activation as measured by granule release markers and platelet aggregation. Taken together, these data suggest that ABT-737 induces an apoptosis-like response in platelets that is distinct from platelet activation and results in enhanced clearance in vivo by the reticuloendothelial system. 相似文献
256.
Nieto M Finn S Loda M Hahn WC 《The international journal of biochemistry & cell biology》2007,39(9):1562-1568
Prostate cancer is the most common, non-dermatologic cancer in men. Since prostate cancer is highly associated with increased age, the incidence of this disease is expected to increase as the population ages. In its initial stages prostate cancer depends upon the actions of androgen, and androgen deprivation therapy induces tumor regression. Currently, androgen deprivation is achieved by either surgical or chemical androgen blockade. Unfortunately, nearly all prostate cancer patients develop tumors that grow despite androgen blockade and ultimately relapse. Many alterations in prostate cancer cells contribute to this state. Although chemotherapy induces short remissions in some patients, there are no curative therapies for metastatic disease. This review summarizes our current understanding in androgen signaling and the mechanisms that allow tumor cells to bypass androgen manipulation therapy. The identification of novel survival pathways and effector molecules that drive androgen independent growth is necessary to develop effective therapies for advanced prostate cancers. 相似文献
257.
We devised a bioinformatics method for systematic identification of putative human-specific exon-deletion mutations that occurred after the divergence of human and chimpanzee and experimentally verified 2 of the predicted mutations in MOXD2 and S100A15A genes. MOXD2 gene encodes a monooxygenase that is highly conserved in mammals and is mostly expressed in the olfactory epithelium in mouse. The presence of a deletion of the last 2 exons and a polymorphic nonsense mutation in exon 6 suggests that MOXD2 gene is inactive in humans. S100A15A is a member of the S100 family of calcium-binding proteins, the mouse ortholog of which is expressed during epidermal maturation. Human S100A15A gene is likely to be inactive because the start codon-bearing exon is deleted in human. We propose that modification or inactivation of MOXD2 and S100A15A genes have contributed to the loss of certain smell sense in humans and to the development of human skin. 相似文献
258.
259.
Hahn Kyu Ri Kim Woosuk Jung Hyo Young Kwon Hyun Jung Kim Dae Won Hwang In Koo Yoon Yeo Sung 《Neurochemical research》2022,47(4):1073-1082
Neurochemical Research - Cuprizone is commonly used to induce neuronal demyelination in mice. In the present study, we compared the cuprizone-induced demyelination in the corpus callosum and... 相似文献
260.