Blockage of IGF－1R signaling sensitizes urinary bladder cancer cells to mitomycin－mediated cytotoxicity

INTRODUCTIONtransitional cell carcinoma (TCC) of the bladder represents the fifth most preValent malignancy inwestern population. A major problem in the management of TCC is the low sensitivity to chemotherapy and the high recu-rrence after transurethral resection, which occupies a large proportion (approximately 40%) among bladder cancer patients[1, 21. Sodrug resistance remains a major and difficult problem to resolye in TCC chemotherapy. This phenomenon has often been ascribed to so…     

桔梗的组织培养

桔梗 (ＰｌａｔｙｃｏｄｏｎｇｒａｎｄｉｆｌｏｒｕｍＡ .ＤＣ .)为桔梗科多年生草本植物 ,根供药用 ,有宣肺、祛痰、排脓之功效。用于治疗外感咳嗽 ,咽喉肿痛 ,肺痈吐脓等症[1] 。近年来 ,桔梗成为极具开发前景的一种药食两用经济作物 ,需求量大增 ,野生资源不能满足需要 ,人工栽培问题已有研究[2 ] ,进展也较快。桔梗药材生产主要采用种子或无性繁殖 ,而通过组织培养的方法进行桔梗快繁 ,在 80年代中期少数学者进行过初步研究[3 ,4 ] 。作者拟通过桔梗的离体培养试验 ,优化培养条件 ,为缩短桔梗育苗周期和优良品种快繁提供依据。1　材料…     

转Bt基因水稻对二化螟幼虫取食、生长及其存活的影响(英文)

在实验室中以转cry1Ab基因水稻 克螟稻 1号为材料 ,研究了不同温度下Bt水稻对二化螟Chilosuppressalis (Walker) 3龄和 5龄幼虫取食、生长及其存活的影响。结果表明 ,不同温度下 3龄和 5龄幼虫取食Bt水稻后 ,其取食量、体重增长和存活率均极显著低于对照。温度对 3龄幼虫取食、生长和存活无显著影响 ,但对 5龄幼虫的取食和体重增长则有显著影响。幼虫死亡率与其取食Bt水稻的累积食量间存在着正相关关系。     

苹果园光能截获率的数学模型

应用气象学原理推导了不同纬度、不同栽植行向、不同树形的苹果园光能截获率的数学模型,给出在保证树冠基部外围日照时间大于25％总日照时间的条件下,生长季中充分截获光能的最佳树形、行距、冠高、冠径的优化组合方案。实例分析表明,计算结果与专家经验基本一致。本研究为果园合理栽植,充分利用光能,合理整形修剪提供理论依据与参考方案。     

生物芯片技术的原理与应用

生物芯片是指将大量生物讯息密码(寡核苷酸、cDNA、基因组DNA、蛋白质等)以预先设计的方式固定在玻片、硅片等固相载体上组成的密集分子阵列,可分为核酸芯片、蛋白芯片、芯片实验室三类.生物芯片技术的本质是生物信号的平行分析,它利用核酸分子杂交、蛋白分子亲和原理,通过荧光标记技术检测杂交或亲和与否,可迅速获得所需信息.高效、快速的生物芯片技术以其无与伦比的优势,已在医学、分子生物学等领域显现出巨大的应用价值,具有非常广阔的发展前景。 Abstract:Bio-chip is a molecular micro-array,which is composed of some biological message codes (e.g.ohgonucleotides,cDNA,genome DNA,protein,and so on) arranged on solid surfaces in light of a engineering design in advance.There are three kinds of Bio-Chips,i.e.,nucleic acid chip,protein chip,and chip “lab”.The essence of Bio-Chip technology is a parallel analysis for biology message.It is based on the principle of nucleic acid molecule hybridization or protein immunochemistry.The occurrence of hybridization/immunoreaction is detected by fluo-labelled technology.Then the needed message can quickly be obtained.Efficient and fast Bio-Chip technology has already exhibited enormous applied value in medical science and molecule biology field because of incomparable superority.Moreover,it possesses very wide development prospect.     

金门浯江溪口鸟类的多样性

在福建省金门岛西岸的滨海滩地浯江溪口进行了为期2年的调查,采用每月2次的定点调查,获得鸟类30科105种的记录,2000年为27科89种5514只;2001年为28科84种9079只,两年种类和数量起伏月变动曲线都有2个高峰,一是在春季,另一是在秋季,虽两年高峰点有前后之差,但曲线的模式,基本上是一致的,滩地的冬候鸟和旅鸟最为显著,优势种包括普通鸬鹚（Phalacrocorax carbo),环颈Xing（Charadrius alexandrinus),白腰杓鹬（Numenius arquata),中杓鹬（Numenius phaeopus),翻石鹬（Arenaria interpres),黑腹滨鹬（Calidris alpina)等,物种多样性指数均以春季最高,秋季次之,夏季最为贫瘠。     

国外松衰退病原因的研究

对安徽省国外松大面积衰退和枯死原因的调查研究表明,造成这种衰退和枯死的现象是多方面因素共同作用的结果,是一种林木衰退病.该衰退病的发生原因:1)诱发因素:温度年较差较大、年降雨量分布不均匀、土壤粘重板结或容重过大、地势低洼积水、土壤瘠薄、林分密度过大;2)激化因素:干旱严重重复发生、割脂过早过度、霜冻、食叶害虫等害虫危害;松针褐斑病等病害侵害;3)促进因素:松枯梢病菌、根腐病菌、立木腐朽病菌、天牛、小蠹虫等病虫害危害.     

以小时为步长的大兴安岭典型林分地表死可燃物含水率模型预测及外推精度

地表死可燃物含水率是火险天气和火行为预报中的重要指标.本研究基于时滞平衡含水率法(Nelson和Simard方法)及气象要素回归方法,于2010年9—10月对黑龙江省大兴安岭地区盘古林场不同郁闭度的山杨-白桦混交林、红皮云杉纯林,以及采伐迹地(原1∶1樟子松-白桦混交林)地表死可燃物含水率进行以小时为步长的连续测定,建立其预测模型,得到预测误差,并使用相应的模型对其他林分地表死可燃物含水率进行外推精度分析.结果表明:采用Nelson平衡含水率法构建的地表死可燃物含水率变化模型的平均绝对误差、平均相对误差和均方误差根(0.0154、0.104和0.0226)低于Simard法(0.0185、0.117和0.0256)和气象要素回归法(0.0222、0.150和0.0331).在外推效果方面,气象要素回归法的平均绝对误差、平均相对误差和均方误差根(0.0410、0.0300和0.0740)低于Simard法(0.610、0.492和0.846),但前两者均高于Nelson法(0.034、0.021和0.0660),说明以小时为步长的时滞平衡含水率法,尤其是Nelson法适用于大兴安岭地区所测林分.外推虽不能降低误差,但有助于提高现有模型应用至不同林分条件或大尺度范围内的地表死可燃物含水率预测精度和利用率.模型建模和外推误差与不同树种和郁闭度条件差异有关,研究时应根据不同林分和地点选择合适的平衡含水率模型.     

A Rapid,Non-invasive Method for Anatomical Observations of Tadpole Vertebrae in Vivo

The tadpole is a critical stage in the amphibian life cycle and plays an important role during the transition from the aquatic to the terrestrial stage.However,there is a large gap in tadpole research,which represents a vital component of our understanding of the diversity and complexity of the life history traits of amphibians,especially their developmental biology.Some aspects of this gap are due to limited research approaches.To date,X-ray microcomputed tomography(micro-CT)has been widely used to conduct osteology research in adult amphibians and reptiles,but little is known about whether this tool can be applied in tadpole studies.Thus,we compared the results of two methods(the bone-cartilage double-staining technique and micro-CT)to study vertebrae in tadpole specimens.The results revealed no significant difference between the two methods in determining the number of vertebrae,and micro-CT represents a rapid,non-invasive,reliable method of studying tadpole vertebrae.When scanning tadpoles,voltage is the most critical of the scanning parameters(voltage,current and scan time),and moderate scanning parameters are recommended.In addition,micro-CT performed better using specimens stored in 70% ethanol than those preserved in 10% formalin.Finally,we suggest that micro-CT should be more widely applied in herpetological research to increase specimen utilization.