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11.
Plant leaf colour is commonly used as an index for nutrientdiagnosis. We have developed a low-cost diagnostic method thatis easy to use to assess the nutrient status of plants, basedon the estimation of chlorophyll content of leaves using a portablecolour video camera and a personal computer. Relationships betweenchlorophyll content and various functions derived from red,green and blue wavelengths are examined. Although red-blue andgreen-blue wavelengths show the highest correlation with chlorophyllcontent under a limited range of meteorological conditions,the normalized difference (red-blue)/(red+blue) is the mostapplicable function which can use data collected under differentmeteorological conditions. The accuracy in estimating chlorophyllcontent from video images could be improved by correcting withsolar radiation data. It is shown that, for practical purposes,the chlorophyll content of leaves can be estimated with sufficientaccuracy using a portable video camera.Copyright 1998 Annalsof Botany Company Chlorophyll content; leaf colour; video camera; image processing; nutrient diagnosis. 相似文献
12.
Fraction 1 protein of spinach and tobacco leaves was purifiedby Sephadex G-200 and DEAE-cellulose column chromatography.Immunological comparison of purified preparations of these proteinswas carried out with precipitin analysis using an antiserumof tobacco fraction 1 protein. Two different antigenic activitieswere found in tobacco fraction 1 protein, of which one was commonlyfound in the spinach protein and the other was specific to tobaccofraction 1 protein. The immunological results suggest that fraction 1 protein iscomposed of at least two different structures, one of whichis common to both spinach and tobacco proteins and the otherof which is specific to each of these proteins. This was confirmedfrom a chemical experiment. Fraction 1 protein was divided intolarge and small polypeptide components by sodium dodecyl sulfatetreatment and subsequent Sephadex G-100 column chromatography,then the amino acid compositions of each polypeptide of theseproteins were compared. The amino acid composition of the smallpolypeptide of spinach was different from that of tobacco, whileamino acid compositions of the large polypeptides of those proteinswere similar to each other. (Received July 1, 1968; ) 相似文献