贻贝棘尾虫镜像骈体横切后前后切块再分别左右对折的实验

将贻贝棘尾虫镜像骈体横断后,再将前后切块的左右半细胞做对折骈接。前半部分切块对折后有６７％成为左右镜像对称骈体;有３３％形成末端对末端的镜像骈体,最后分开成为一正常虫体和一具有反向口围的单体虫。后半部分切块对折后基本上全部形成左右镜像对称骈体。这一事实可用位置值的添加理论来解释。但也显示出预存的缘棘毛在确定形成左右镜像对称骈体或末端对末端的镜像骈体中所起的重要作用。     

图们江流域河流生态系统健康评价

河流是重要的自然生态系统,也是重要的生态廊道之一,图们江流域河流生态系统的健康状况,对维护跨国界流域的水环境的管理和可持续发展有着重要的意义。基于河流水文、河流形态、河岸带状况、水体理化参数以及河流生物5个层面选取22个指标构建了图们江流域河流生态系统健康评价指标体系,运用层次分析法和加权平均法对其进行了健康评价。评价结果表明,虽然该地区处于"健康"级别,但也有28%的地区处于"亚健康"状态。河流健康综合指数(RHI`)与河流生物指标、水体理化指标、河流形态指标、河岸带指标等4项呈显著相关(P0.05),相关系数依次为0.847、0,757、0.740、0.547。研究结果表明图们江流域水生生物的生存环境遭到严重破坏、水体污染严重,河岸带生态退化、城市化影响严重等一系列影响河流健康的问题。     

人肿瘤坏死因子α基因穿梭表达载体的构建和在鱼腥藻7120中的表达

报道了人肿瘤坏死因子α(hTNFα)基因穿梭表达载体的构建及其在丝状体蓝藻鱼腥藻 712 0中的表达和鉴定结果 .将质粒pRL rhTNF上hTNFα的cDNA连于pRL 43 9质粒上的PpsbA启动子下游 ,得到中间载体pRL TC .进一步与穿梭质粒pDC 8重组 ,得到可在大肠杆菌和蓝藻中均可表达的穿梭表达载体pDC TNF .pRL rhTNF ,pRL TC和pDC TNF三者在大肠杆菌中的表达量分别为 11.8% ,16 9%和 15 .0 % .通过三亲接合转移将pDC TNF引入鱼腥藻 712 0中并获稳定遗传的转基因株 .从转基因的鱼腥藻 712 0中检测到pDC TNF质粒的存在 ,且在和TNFα的cDNA探针进行的Southern杂交中呈阳性反应 .抽提转基因藻的蛋白样品进行检测 ,在Western印迹中和TNFα单克隆抗体呈阳性反应 .采用TNF对L92 9细胞的细胞毒性方法 ,证明转基因藻粗提液中 ,TNF确有细胞毒活性 .     

Resistance of SKW6 cell to apoptosis induction with anti-Fas antibody upon transduction of a reverse fragment to a cDNA encoding human 6A8 α-mannosidase

The effect of transduction with a reverse fragment to a cDNA encoding human 6A8 α-mannosidase on apoptosis induction of human B cell line SKW6 by anti-Fas antibody was tested. Apoptosis-inducer of anti-Fas monoclonal antibody was used to induce apoptosis in SKW6 cells. Giemsa’s staining, Annexin-V-FLUOS staining and DNA ladder test were used to determine the events of apoptosis. Indirect immunofluorescent staining with anti-Fas antibody was performed to detect the surface Fas expression. In a time-course test of 12, 24 and 36 h for apoptosis induction by anti-Fas antibody, DNA ladder was observed in the wild-type SKW6 cells in a time-dependent fashion. Mock transduction had no effect on DNA ladder production. However, no DNA ladder was detected in the rAAV-antisense 6A8 cDNA-transduced SKW6. Results from Annexin-V-FLUOS staining on anti-Fas antibody-treated cells revealed that the staining-positive rate in the rAAV-antisense 6A8 cDNA-transduced SKW6 cells was decreased in comparison to that in the wild-type and the mock-transduced cells. Giemsa’s staining observation showed that the number of dying (with apoptotic bodies) and dead cells was reduced in the rAAV-antisense 6A8 cDNA-transduced SKW6 cells in comparison with that in the wild-type and the mock-transduced cells upon anti-Fas antibody induction. The transduction did not affect the expression of Fas molecular on cell surface. 100% cells in all the groups showed Fas expression. The SKW6 cells became resistant to apoptosis induction by anti-Fas antibody upon transduction with a reverse fragment to a cDNA encoding human 6A8 α-mannosidase. The transduction did not affect the expression of Fas molecule on cells.     

莼菜冬芽越冬生理研究

按季节测定了莼菜生长周期呵溶性蛋白质、可溶性糖、脯氨酸以及水分的含量,测定结果表明冬芽越冬阶段其体现人可溶性蛋白质、可溶性９糖以及水分的含量较高,而脯氨酸却是一年中最低。这说明冬芽冬越期间其抗寒性的维持可能与体内高浓度的可溶性蛋白质,可溶性糖以及水分有关,而与脯氨酸无直接的关系。     

Using yeast two-hybrid system to detect interactions of ATP synthase subunits from Spinacia oleracea

Subunit interactions among the chloroplast ATP synthase subunits were studied using the yeast two-hybrid system. Various pairwise combinations of genes encoding a, p, y, 8 and e subunits of Spinach ATP synthase fused to the binding domain or activation domain of GAL4 DNA were introduced into yeast and then expression of a reporter gene encoding p-galactosidase was detected. Of all the combinations, that of y and e subunit genes showed the highest level of reporter gene expression, while those of a and p, a and e, p and e and p and 8 induced stable and significant reporter gene expression. The combination of 8 and e as well as that of 8 and y induced weak and unstable reporter gene expression. However, combinations of a and y, p and y and a and 8 did not induce reporter gene expression. These results suggested that specific and strong interactions between y and e, a and p, a and e, p and e and p and 8 subunits, and weak and transient interactions between 8 and e and 8 and y subunits occurred in the yeast     

破伤风毒素C部分的克隆及在大肠杆菌中的表达

用ＣＲ方法从破伤风杆菌基因组ＤＮＡ上坟增出破伤风毒素Ｃ部分（ｔｅｔａｎｕｓ ｔｏｘｉｎ ｆｒａｇｍｅｎｔ Ｃ,ＴＴＣ）,经测序其基因片段由１３５６ｂｐ组成,可编码４５１个氨基酸残基的多肽。将其插入原核表达载体ｐＥＴ－２８ａ（＋）中,并在大肠杆力ＢＬ２１９ＤＥ３）中表达,其表达量占可溶性总蛋白质的８．２％;ＳＤＳ－ＰＡＧＥ和免疫印迹分析表明,ＴＴＣ基因（该基因ＧｅｎＢａｎｋ登录号为ＡＦ１５４８２）表     

突变的叶绿体ATP合成酶ε亚基对菠菜叶绿体毫秒延迟发光的影响

突变和野生型叶绿体ＡＴＰ 合成酶的ε亚基均可增强叶绿体毫秒延迟发光的快相;不同的突变体对快相的增强强度不同,但与它们对离体ＣＦ１ 的Ｃａ２＋ＡＴＰ酶水解活力的抑制程度情况一致。且这种影响在１ ℃左右的低温下较显著,而温度升至２５ ℃时,这种增强作用趋于消失。加入解联剂后,快相部分消除,ε亚基仍有增强作用。这些结果说明,ε亚基是通过与ＣＦ１ 的专一作用而影响光系统Ⅱ附近类囊体膜的动态结构, 使质子不易从类囊体膜上流失,质子动力势较难被解联剂所去除     

精神病患者家属心理健康状况调查研究

目的:探讨精神病患者家属的心理健康状况,并找出相关的影响因素.方法:对中山市91位住院以及门诊精神科病人家属进行问卷调查和访谈,采用的是症状自评量(SCL-90)和家庭疾病负担量表(Family Burden Scale of Disease).结果:家属心理健康状况与患者给家庭带来的负担存在明显的相关,患者患病对配偶的心理健康的影响明显大于对其他亲人或者朋友的影响(F=5.358,P＜0.001),15-30岁年龄段的SCL-90的得分要明显的高于其他年龄段(F=1.436,P＜0.01),而精神分裂症患者的家属SCL-90的得分也要明显高于其他疾病家属的得分.结论:影响家属心理健康的因素有多个方面,应该有针对性的采取家庭护理措施并加强家庭健康教育.     

皱边石杉内生真菌DNA提取有效方法比较

目的:优化确定适用于皱边石杉内生真菌DNA提取的有效方法,并评价其效果。方法:运用氯化苄改良法、CTAB改良法分别提取皱边石杉内生真菌菌丝体DNA。结果:氯化苄改良法可从17个内生真菌菌丝体中均获得了高质量的DNA,除5、13、14号生长缓慢的内生真菌其菌丝体DNA浓度≤60ng/μL,其余样品的DNA浓度均≥200ng/μL。而CTAB改良法仅适宜于平板培养菌落生长迅速、菌落疏松,发酵培养菌丝体产量高的1、2、3、9、10、12号菌株。结论:氯化苄改良法是适宜于皱边石杉内生真菌DNA提取的理想方法。