Structures of cytokinins influence synergistic production of ethylene

Twenty-five naturally occurring cytokinins and structurally related compounds were tested for their ability to promote ethylene production synergistica     

新疆副新蚤属一新种记述(蚤目:毛蚤科)

球副新蚤Paraneopsylla globa新种 鉴别特征 本新种与深窦副新蚤(P.ioffi Tiflov,1937)的形态比较相似,其主要区别是新种第9腹板后臂前缘近端处明显前凸,后缘虽微凹但未形成深窦,端部纯圆,未形成背角;阳茎钩突除端部膜状增宽外,基部尚赘生大的球形膜状物。新种♀第7腹板端缘上叶突出比较狭小。 形态记述 头部(图1)额瘤齿状,嵌于额缘近下1/4处。下唇须约达前足基节长的3/4—4/5。额宗列3—4支宗,均较细小。眼宗列3支宗,较粗长。后头宗3列,依次为1—2、2—3、5—6支,前两列除下位宗外均较细小。 胸部(图1):前胸栉两侧共有栉刺14支,栉前有一列宗4—6支。中胸背板有两列较完整的宗,其前尚有不少小宗,颈片每侧具假宗1—3支。 部分标本后胸背板后缘有1—2个端齿。前足基节外面有宗40—50支。后足基节内面有细宗25—40支,多位于近     

Ginsenoside Rg1 protects H9c2 cells against nutritional stress-induced injury via aldolase /AMPK/PINK1 signalling

Insufficient nutrients supply will greatly affect the function of cardiac myocytes. The adaptive responses of cardiac myocytes to nutritional stress are not fully known. Ginsenoside Rg1 is one of the most pharmacologically active components in Panax Ginseng and possesses protective effects on cardiomyocyte. Here, we investigate the effects of ginsenoside Rg1 on H9c2 cells which were subjected to nutritional stress. Nutritional stress-induced by glucose deprivation strongly induced cell death and this response was inhibited by ginsenoside Rg1. Importantly, glucose deprivation decreased intracellular ATP levels and mitochondrial membrane potential. Ginsenoside Rg1 rescued ATP levels and mitochondrial membrane potential in nutrient-starved cells. For molecular mechanisms, ginsenoside Rg1 increased the expressions of PTEN-induced kinase 1 (PINK1) and p-AMPK in glucose deprivation treated H9c2 cells. Reducing the expression of aldolase in H9c2 cells inhibited ginsenoside Rg1′s actions on PINK1 and p-AMPK. Further, the nutritional stress mice were used to verify the mechanisms obtained in vitro. Ginsenoside Rg1 increased the expressions of aldolase, p-AMPK, and PINK1 in starved mice heart. Taken together, our results reveal that ginsenoside Rg1 limits nutritional stress-induced H9c2 cells injury by regulating the aldolase /AMP-activated protein kinase/PINK1 pathway.     

Comprehensive profiling of accessible surface glycans of mammalian sperm using a lectin microarray

It is well known that cell surface glycans or glycocalyx play important roles in sperm motility, maturation and fertilization. A comprehensive profile of the sperm surface glycans will greatly facilitate both basic research (sperm glycobiology) and clinical studies, such as diagnostics of infertility. As a group of natural glycan binders, lectin is an ideal tool for cell surface glycan profiling. However, because of the lack of effective technology, only a few lectins have been tested for lectin-sperm binding profiles. To address this challenge, we have developed a procedure for high-throughput probing of mammalian sperm with 91 lectins on lectin microarrays. Normal sperm from human, boar, bull, goat and rabbit were collected and analyzed on the lectin microarrays. Positive bindings of a set of ~50 lectins were observed for all the sperm of 5 species, which indicated a wide range of glycans are on the surface of mammalian sperm. Species specific lectin bindings were also observed. Clustering analysis revealed that the distances of the five species according to the lectin binding profiles are consistent with that of the genome sequence based phylogenetic tree except for rabbit. The procedure that we established in this study could be generally applicable for sperm from other species or defect sperm from the same species. We believe the lectin binding profiles of the mammalian sperm that we established in this study are valuable for both basic research and clinical studies.     

上海农作物种质资源数据库的设计和构建

随着农作物种质资源数量的不断增加,种质资源交流的日益频繁以及种质资源利用的逐步深入,农作物种质资源数据库的建设对农作物的应用研究和基础性研究至关重要。上海市农业生物基因中心借助于本中心保存的近21万份种质资源的信息,开发了一套上海农作物种质资源数据库,本文主要阐述了该数据库设计和构建的目的、原则和数据库的架构、运行环境以及主要功能模块。上海农作物种质资源数据库采用了B/S结构,平台系统利用多层逻辑架构实现了对农作物种质资源数据信息的收集、存储、查询、统计分析等功能。外部用户通过浏览器即可访问平台系统,浏览查询种质资源信息。内部管理员可使用平台的受限功能,需要认证成功后才可使用后台管理功能,实现对整个平台数据的管理。注册用户还可通过基因资源数据库提交引种申请,管理员结合管理信息系统实现对外供种全流程的网络化,借此可提高工作效率,增加工作流程的透明度。     

Room Temperature Preparation of Surface-Clean Hydrogen-Doped Plasmonic Molybdenum Oxide as a High-Efficient and Degradable Reactive Oxygen Species Scavenger

Considerable efforts have been made to develop reactive oxygen species (ROS) scavengers for removing high level of ROS. However, most of the reported ROS scavengers are nondegradable and involve harsh reaction conditions as well as utilize various surface ligands. In order to overcome these drawbacks, in the present work, we develop a facile and mild synthesis avenue for preparation of surface-clean hydrogen-doped molybdenum oxide (H_0.34MoO₃) via simply mixing MoO₃ dispersion with aluminum foil under an acidic environment without any surface capping reagents at room temperature. The resulting H_0.34MoO₃ can act as a broad-spectrum ROS scavenger, including ^.OH, H₂O₂, O₂⁻, and ¹O₂ as well as 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The free radical scavenging activity of H_0.34MoO₃ achieves as high as 71.6% and 99.1% for ^.OH and DPPH scavenging, which is comparable and superior to that of ascorbic acid that is a classic free radical scavenger. More significantly, the resulting H_0.34MoO₃ is degrade, which can be degraded into molybdate ions under a neutral environment (pH 7.4).