利用16S rRNA基因同源性分析鉴定两株明串珠菌

从酸马奶中分离出2株明串珠菌KLDS 5.0301和KLDS 5.0302,对2株菌的16S rRNA基因经PCR扩增测序,将测序结果同该属内菌株的16S rRNA序列作多序列比较,并建立明串珠菌属的系统发育树.结果表明,KLDS 5.0301的16S rRNA序列同L. garlicum的同源性百分比为100%.KLDS 5.0302的16S rRNA序列同L.mesenteroides LM2菌株的16S rRNA序列的同源性百分比为99.9%.根据系统发育树的结果,将KLDS5.0301鉴定为L.garlicum,KLDS 5.0302鉴定为L.mesenteroides.菌株KLDS 5.0301和KLDS 5.0302的16SrRNA序列已经在GeneBank申请国际序列注册号,分别为DQ239691和DQ297412.     

我国生态文明建设及其评估体系研究进展

传统的资源利用和环境管理方式已经越来越不能适应人们对于物质文明和精神文明的追求,资源节约型和环境友好型社会是现代区域规划的新需求,生态文明建设是体现这一理念的必然选择。全面认识和理解生态文明建设方法及其跟踪评估机制是加强生态文明建设的前提,但目前研究中提供给管理者的信息还很有限。目前国内对生态文明建设的研究主要集中在3个方面:生态文明建设内涵、生态文明建设指标体系和生态文明建设跟踪评估指标体系。综述了国内当前生态文明建设主要特征,采用评价指标重要值的方法评估生态文明建设和评估指标选取的重要性程度,提出了生态文明建设理论框架、建设与跟踪评估指标体系和今后的研究方向。     

蝗卵的研究Ⅱ.蝗卵在孵育时的变化及其意义

一.引言 东亚飞蝗在卵階段的发育过程有许多地方是和其他种类的昆虫相类似的,但是也有许多地方可作为飞蝗种的特徵。蝗虫是陸棲的昆虫,它们的卵经常在土中孵育,因此在卵的生理和构造上有许多与此种生活习性相适应的特点。和其他昆虫的卵一样,蝗卵是一种有壳卵(cleidoic egg),但含有更为充分的营养物质。它外面的卵包膜     

人β神经生长因子基因稀有密码子及mRNA二级结构对其在大肠杆菌中表达的影响

目的:研究人β神经生长因子(β-NGF)基因中稀有密码子及其mRNA二级结构对其在大肠杆菌中表达量的影响。方法:根据对人β-ngf中稀有密码子及其mRNA二级结构的研究,同义突变人β-ngf基因,通过PCR得到人β-ngf的5’端同义突变基因rh-β-ngfp32和全同义突变基因rh-β-ngfmu,将这2个序列克隆入载体pET3a中,得到重组质粒pET3a-ngfp32和pET3a-ngfmu,分别转化大肠杆菌BL21(DE3)感受态,IPTG诱导表达,收集菌体,SDS-PAGE检测其表达量的改变。结果:构建的pET3a-ngfp32和pET3a-ngfmu表达载体酶切和测序结果正确,SDS-PAGE结果显示,与在重组菌pET3a-NGF总蛋白中的表达量相比,目的蛋白rh-β-NGF在重组菌pET3a-NGFP32和pET3a-NGFmu中的表达量均明显增高,并且在重组菌pET3a-NGFmu中的表达量高于重组菌pET3a-NGFP32。结论:目的蛋白rh-β-NGF在重组菌pET3a-NGFP32和pET3a-NGFmu中表达量的增高,说明人β-ngf基因中稀有密码子和mRNA的二级结构对其在大肠杆菌中的表达有较为明显的影响,结果为构建rh-β-NGF的大肠杆菌工程菌株奠定了基础。     

施肥对西双版纳橡胶林土壤CO_2排放的影响

对土地的人工管理影响到土壤CO2的排放。西双版纳是中国橡胶主要种植区,施肥是橡胶林获取更好效益的主要管理措施之一。为探讨施肥对橡胶林土壤CO2排放的影响,选取西双版纳大面积种植的橡胶林,采用静态箱-气相色谱法开展了不同施肥处理试验。结果表明:(1)施肥处理没有改变西双版纳橡胶林土壤CO2排放的时间变化特征;(2)不同施肥处理和对照的西双版纳橡胶林土壤CO2排放均呈现出雨季大于干季的趋势,年排放总量在7.88~7.93 t C·hm-2·a-1;(3)不同施肥处理的土壤CO2月排放量大于对照;(4)施肥处理在一定程度上可促进土壤呼吸,使土壤CO2排放增加,但差异不显著(P0.05)。本研究结果为进一步揭示西双版纳橡胶林生态系统碳循环过程及其机理提供支持,为把握橡胶林碳平衡动态和应对全球变化提供参考,也可为橡胶林的可持续利用提供科学依据。     

C1 metabolism and the Calvin cycle function simultaneously and independently during HCHO metabolism and detoxification in Arabidopsis thaliana treated with HCHO solutions

Formaldehyde (HCHO) is suggested to be detoxified through one‐carbon (C1) metabolism or assimilated by the Calvin cycle in plants. To further understand the function of the Calvin cycle and C1 metabolism in HCHO metabolism in plants, HCHO elimination and metabolism by Arabidopsis thaliana in HCHO solutions was investigated in this study. Results verified that Arabidopsis could completely eliminate aqueous HCHO from the HCHO solutions. Carbon‐13 nuclear magnetic resonance (¹³C‐NMR) analysis showed that H¹³CHO absorbed by Arabidopsis was first oxidized to H¹³COOH. Subsequently, a clear increase in [U‐¹³C]Gluc peaks accompanied by a strong enhancement in peaks of [2‐¹³C]Ser and [3‐¹³C]Ser appeared in Arabidopsis. Pretreatment with cyclosporin A or L‐carnitine, which might inhibit the transport of ¹³C‐enriched compounds into chloroplasts and mitochondria, caused a remarkable decline in yields of both [U‐¹³C]Gluc and [3‐¹³C]Ser in H¹³CHO‐treated Arabidopsis. These results suggested that both the Calvin cycle and the C1 metabolism functioned simultaneously during HCHO detoxification. Moreover, both functioned more quickly under high H¹³CHO stress than low H¹³CHO stress. When a photorespiration mutant was treated in 6 mm H¹³CHO solution, formation of [U‐¹³C]Gluc and [2‐¹³C]Ser was completely inhibited, but generation of [3‐¹³C]Ser was not significantly affected. This evidence suggested that the Calvin cycle and C1 metabolism functioned independently in Arabidopsis during HCHO metabolism.     

中国微蛛三新种和一新纪录（蜘蛛目：皿蛛科）

本文记述采自长白山区皿蛛科微蛛亚科３新种和１新纪录种。新种包括：钩镰蛛Ｄｒｅｐａｎｏｔｙｌｕｓ ａｄｕｎｃｕｓ ｓｐ．ｎｏｖ．，圆双突蛛Ｔｉｂｉｏｐｌｏｉｄｅｓ ｃｙｃｌｉｃｕｓ ｓｐ．ｎｏｖ．和具结荫湿蛛Ｈｉｌａｉｒａ ｔｕｂｅｒｃｕｌｉｆｅｒａ ｓｐ．ｎｏｖ．。模式标本均保存于白求恩医科大学生物教研室。