全文获取类型
收费全文 | 91篇 |
免费 | 9篇 |
国内免费 | 2篇 |
出版年
2022年 | 1篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2017年 | 5篇 |
2015年 | 5篇 |
2014年 | 7篇 |
2013年 | 4篇 |
2012年 | 7篇 |
2011年 | 2篇 |
2010年 | 4篇 |
2009年 | 4篇 |
2008年 | 2篇 |
2007年 | 2篇 |
2006年 | 4篇 |
2005年 | 5篇 |
2004年 | 4篇 |
2003年 | 1篇 |
2002年 | 3篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 5篇 |
1973年 | 1篇 |
1970年 | 2篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1934年 | 1篇 |
1929年 | 1篇 |
排序方式: 共有102条查询结果,搜索用时 31 毫秒
51.
Maggie?A. Ostrowski Ngan?F. Huang Travis?W. Walker Tom Verwijlen Charlotte Poplawski Amanda?S. Khoo John?P. Cooke Gerald?G. Fuller Alexander?R. Dunn 《Biophysical journal》2014,106(2):366-374
At present, little is known about how endothelial cells respond to spatial variations in fluid shear stress such as those that occur locally during embryonic development, at heart valve leaflets, and at sites of aneurysm formation. We built an impinging flow device that exposes endothelial cells to gradients of shear stress. Using this device, we investigated the response of microvascular endothelial cells to shear-stress gradients that ranged from 0 to a peak shear stress of 9–210 dyn/cm2. We observe that at high confluency, these cells migrate against the direction of fluid flow and concentrate in the region of maximum wall shear stress, whereas low-density microvascular endothelial cells that lack cell-cell contacts migrate in the flow direction. In addition, the cells align parallel to the flow at low wall shear stresses but orient perpendicularly to the flow direction above a critical threshold in local wall shear stress. Our observations suggest that endothelial cells are exquisitely sensitive to both magnitude and spatial gradients in wall shear stress. The impinging flow device provides a, to our knowledge, novel means to study endothelial cell migration and polarization in response to gradients in physical forces such as wall shear stress. 相似文献
52.
53.
54.
55.
Kopa Paulina Macieja Anna Gulbas Izabela Pastwa Elzbieta Poplawski Tomasz 《Molecular biology reports》2020,47(1):67-76
Molecular Biology Reports - Etoposide (VP-16) is the topoisomerase 2 (Top2) inhibitor used for treating of glioma patients however at high dose with serious side effects. It induces DNA... 相似文献
56.
Szczepanska J Poplawski T Synowiec E Pawlowska E Chojnacki CJ Chojnacki J Blasiak J 《Molecular biology reports》2012,39(2):1561-1574
HEMA (2-hydroxyethyl methacrylate), a methacrylate commonly used in dentistry, was reported to induce genotoxic effects, but
their mechanism is not fully understood. HEMA may be degraded by the oral cavity esterases or through mechanical stress following
the chewing process. Methacrylic acid (MAA) is the primary product of HEMA degradation. In the present work we compared cytotoxic
and genotoxic effects induced by HEMA and MAA in human gingival fibroblasts (HGFs). A 6-h exposure to HEMA or MAA induced
a weak decrease in the viability of HGFs. Neither HEMA nor MAA induced strand breaks in the isolated plasmid DNA, but both
compounds evoked DNA damage in HGFs, as evaluated by the alkaline comet assay. Oxidative modifications to the DNA bases were
monitored by the DNA repair enzymes Endo III and Fpg. DNA damage induced by HEMA and MAA was not persistent and was removed
during a 120 min repair incubation. Results from the neutral comet assay indicated that both compounds induced DNA double
strand breaks (DSBs) and they were confirmed by the γ-H2AX assay. Both compounds induced apoptosis and perturbed the cell
cycle. Therefore, methacrylic acid, a product of HEMA degradation, may be involved in its cytotoxic and genotoxic action. 相似文献
57.
添加玉米和水稻秸秆对淹水土壤pH、二氧化碳及交换态铵的影响 总被引:4,自引:0,他引:4
通过温室土壤培养试验,研究了不同添加量玉米和水稻秸秆对淹水土壤pH、CO2及交换态铵的影响.结果表明:在接近中性的土壤中加入秸秆可使土壤pH值降低,4 g·kg-1玉米和水稻秸秆处理的土壤pH值均与对照差异显著(P<0.05),而1 g·kg-1玉米和水稻秸秆处理与对照差异不显著(P>0.05).土壤溶液中的CO2含量随秸秆添加量的增加而增大,1 g·kg-1玉米和水稻秸秆处理的土壤溶液CO2含量最大值分别为35.9%和31.9%(v/v),与对照(25.8%)差异达显著水平(P<0.05),但两者间差异不显著(P>0.05);4 g·kg-1玉米和水稻秸秆处理的土壤溶液CO2含量最大值分别为54.2%和41.8%(v/v),与对照差异极显著(P<0.01),两者间差异也达显著水平(P<0.05).在不施氮肥的情况下,添加秸秆可降低土壤铵态氮浓度,且铵态氮浓度随秸秆添加量的增加而降低,不同添加量处理间差异显著(P<0.05);在施入氮肥的情况下,1 g·kg-1玉米和水稻秸秆处理提高了土壤铵态氮浓度,而4 g·kg-1玉米和水稻秸秆处理降低了土壤铵态氮浓度.无论是否施入氮肥,玉米和水稻秸秆处理的土壤铵态氮浓度差异不显著(P>0.05). 相似文献
58.
MARIA-MARCELA POVEDA MARIE-CLAUDE SOUQUAL MARIE-THÉRÈSE FAUVEL JACQUES GAMISANS THIERRY GAUQUELIN 《Botanical journal of the Linnean Society. Linnean Society of London》2002,140(2):165-168
The cuticular wax composition of leaves has been analysed in three western European populations (Corsica, central Pyrenees, northern Alps) of Juniperus communis var. saxatilis Pall. (= J. nana Willd., nom illeg.) and in one population of J. communis L. var. depressa Pursh. from North America (Sierra Nevada). Gas chromatography shows the presence of 13 alkanes in all samples ranging from C23 to C35 with important intraspecific polymorphism in alkane content. The dominant alkanes range from C33 to C35 . Alkanes C21 and C22 were found only in Corsica and Sierra Nevada populations. Canonical discriminant analysis separated the J. communis L. var. depressa Pursh. of the population of Sierra Nevada from other populations of J. communis var. saxatilis Pall. on the basis of their higher C31 content and the constant presence of C21 and C22 alkanes. J. communis var. saxatilis Pall. populations from the Pyrenees are close to northern Alps populations characterized by high concentrations of C33 , C34 and C35 alkanes. This paper confirms the existence of Juniperus var. saxatilis Pall. in the Pyrenees (France). © The Linnean Society of London, Botanical Journal of the Linnean Society , 2002, 140 , 165–168. 相似文献
59.
Phenotypically concordant and discordant monozygotic twins display different DNA copy-number-variation profiles
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bruder CE Piotrowski A Gijsbers AA Andersson R Erickson S Diaz de Ståhl T Menzel U Sandgren J von Tell D Poplawski A Crowley M Crasto C Partridge EC Tiwari H Allison DB Komorowski J van Ommen GJ Boomsma DI Pedersen NL den Dunnen JT Wirdefeldt K Dumanski JP 《American journal of human genetics》2008,82(3):763-771
The exploration of copy-number variation (CNV), notably of somatic cells, is an understudied aspect of genome biology. Any differences in the genetic makeup between twins derived from the same zygote represent an irrefutable example of somatic mosaicism. We studied 19 pairs of monozygotic twins with either concordant or discordant phenotype by using two platforms for genome-wide CNV analyses and showed that CNVs exist within pairs in both groups. These findings have an impact on our views of genotypic and phenotypic diversity in monozygotic twins and suggest that CNV analysis in phenotypically discordant monozygotic twins may provide a powerful tool for identifying disease-predisposition loci. Our results also imply that caution should be exercised when interpreting disease causality of de novo CNVs found in patients based on analysis of a single tissue in routine disease-related DNA diagnostics. 相似文献
60.