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ANTONY J. MUKKADA FRANK W. SCHAEFER MICHAEL W. SIMON CAROL NEU 《The Journal of eukaryotic microbiology》1974,21(2):393-397
Leishmania tropica promastigotes do not utilize glucose provided in the medium until late log phase. Rapid depletion of glucose from the medium, however, occurs during late log and stationary phases. At about the same time, the cells show maximal rates of glucose uptake as well as peak levels of phosphofructokinase and pyruvate kinase activities. The glucose analog, 2-deoxy-D-glucose inhibits glucose transport. Incorporation of this analog in the growth medium results in inhibition of growth. The hexokinase of L. tropica phosphorylates 2-deoxy-D-glucose. Pyruvate kinase is activated by fructose-1, 6-diphosphate and adenosine monophosphate. 相似文献
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FRANK W. SCHAEFER ELMER MARTIN ANTONY J. MUKKADA 《The Journal of eukaryotic microbiology》1974,21(4):592-596
Transport of glucose by Leishmania tropica promastigotes was measured by the uptake of the nonutilizable glucose analog, 2-deoxy-D-glucose (2-DOG), using the rapid filtration method. Both D-glucose and 2-DOG show identical rates of initial uptake. Intracellular 2-DOG readily exchanges with extracellular D-glucose and 2-DOG uptake is competitively inhibited by D-glucose. These observations suggest that both sugars are taken up by the same system. Neither the glucose analog α-methyl-D-glucoside (α-MG) nor 3-0-methyl glucose (3-0-MG) is taken up to any appreciable extent. Transport of 2-DOG shows saturation kinetics with a Vmax of 3.2 nmoles/mg cells/min and a Km of 0.16 mM. There is thus a stereospecific, carrier-mediated transport system for glucose uptake in L. tropica. About 2/3 of the intracellular pool following transport consists of 2-deoxy-D-glucose phosphate (2-DOG-P) and the remainder is free, unaltered 2-DOG. 相似文献
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A new species of the genus Haminaca has been found in samplesfrom the northern Adriatic Sea. The new species is very similarto H. orteai, but may be clearly distinguished by body size,the non-pigmented area around the eyes, number of leaves ofHancock's organ, number of ridges on the gizzard plates andby the morphology of male genital tract. A comparison and adiscussion of the values of the most significant characterswithin the genus Haminaca is provided herein. (Received 19 September 1991; accepted 28 February 1992) 相似文献