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81.
LEONARD B. THIEN ERIK G. ELLGAARD MARGARET S. DEVALL SARA E. ELLGAARD PAUL F. RAMP 《Plant Species Biology》1994,9(1):47-55
Abstract The population structure and reproductive biology of Saururus cernuus (Lizard's Tail; Saururaceae; Piperales), is documented in five sites in southern Louisiana (Mississippi Delta). The species is common throughout the southeastern United States in marshes, along streams, edges of lakes, and in the understory of moist forests. The clonal species exhibits sexual and vegetative reproduction. Wind and insects both play important roles in pollination. Pollen may be borne by insects directly. Alternatively, the pollen may be borne by wind after its release is triggered from pendulous floral spikes (the “Lizard's Tail”) by either wind or insect landings (insect-mediated wind pollination). The plants are self-incompatible and seed set results from a combination of pollination modes with wind pollination being the primary mode (rare in the Magnoliidae). Inflorescence and floral structure exhibit adaptive features that facilitate the various modes of pollen transfer, viz., numerous, small scented, protogynous flowers with no perianth, ultraviolet patterns produced by stamen filaments, small pollen grains, curved inflorescences, and exerted stamens, etc. Fruit production and seed germination are documented and plant growth and densities are compared in sunny versus forest sites. 相似文献
82.
Secretion of Specific Extracellular Proteins by Somatic Embryos of Picea abies is Dependent on Embryo Morphology 总被引:1,自引:0,他引:1
Embryogenic cell lines ofPicea abieswere categorized into twogroups, A and B, based on the morphology of the somatic embryosand the ability of the somatic embryos to proceed through amaturation process when treated with ABA. Group A embryos hada distinct, densely-packed embryonic region whereas group Bembryos had loosely packed cells in their embryonic region.Embryo morphology was shown to be regulated by changes in theplant growth regulators in the culture medium. Treatment withN6-benzyladenine stimulated embryos to develop large embryonicregions. The morphology of somatic embryos and especially thatof the embryonic regions was correlated with the presence ofspecific extracellular proteins. Only somatic embryos with denselypacked cells in the embryonic regions secreted proteins withrelative molecular weights of 28, 66 and 85kD. The extracellularprotein of 28kD was isolated and the first 21 amino acids inthe N-terminus were identified. These showed 5257% identitywith the N-terminal sequence conserved among members of a proteinfamily which includes zeamatin and which have been shown tobe involved in plant anti-fungal mechanisms. Immunological studiesof extracellular chitinases and zeamatin-like proteins, as wellas of activity of extracellular peroxidase, revealed a closecorrelation between the presence of specific chitinases andembryo morphology. Auxin; cytokinin; embryogenic cell lines; embryo morphology; extracellular proteins; Norway spruce; Picea abies; somatic embryos 相似文献
83.
Temperature effects on forest herbs assessed by warming and transplant experiments along a latitudinal gradient 总被引:1,自引:0,他引:1
PIETER De FRENNE JÖRG BRUNET ANNA SHEVTSOVA ANNETTE KOLB OLIVIER CHABRERIE SARA AO COUSINS GUILLAUME DECOCQ AN De SCHRIJVER MARTIN DIEKMANN ROBERT GRUWEZ THILO HEINKEN MARTIN HERMY CHRISTER NILSSON WESLEY TACK JUSTIN WILLAERT KRIS VERHEYEN 《Global Change Biology》2011,17(10):3240-3253
Slow‐colonizing forest understorey plants are probably not able to rapidly adjust their distribution range following large‐scale climate change. Therefore, the acclimation potential to climate change within their actual occupied habitats will likely be key for their short‐ and long‐term persistence. We combined transplant experiments along a latitudinal gradient with open‐top chambers to assess the effects of temperature on phenology, growth and reproductive performance of multiple populations of slow‐colonizing understorey plants, using the spring flowering geophytic forb Anemone nemorosa and the early summer flowering grass Milium effusum as study species. In both species, emergence time and start of flowering clearly advanced with increasing temperatures. Vegetative growth (plant height, aboveground biomass) and reproductive success (seed mass, seed germination and germinable seed output) of A. nemorosa benefited from higher temperatures. Climate warming may thus increase future competitive ability and colonization rates of this species. Apart from the effects on phenology, growth and reproductive performance of M. effusum generally decreased when transplanted southwards (e.g., plant size and number of individuals decreased towards the south) and was probably more limited by light availability in the south. Specific leaf area of both species increased when transplanted southwards, but decreased with open‐top chamber installation in A. nemorosa. In general, individuals of both species transplanted at the home site performed best, suggesting local adaptation. We conclude that contrasting understorey plants may display divergent plasticity in response to changing temperatures which may alter future understorey community dynamics. 相似文献
84.
SARA B. PRUSS HANNAH CLEMENTE MARC LAFLAMME 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2012,45(3):401-410
Pruss, S.B., Clemente, H. & Laflamme, M. 2012: Early (Series 2) Cambrian archaeocyathan reefs of southern Labrador as a locus for skeletal carbonate production. Lethaia, Vol. 45, pp. 401–410. Archaeocyathan reefs, the first reefs produced by animals, are prominent, global features of early Cambrian successions. However, microbialites – the dominant reef components of the Proterozoic – were still abundant in most archaeocyathan reefs. Although such reefs were a locus for carbonate production, it is unclear how much carbonate was produced skeletally. This analysis of well‐known early Cambrian archaeocyathan patch reefs of the Forteau Formation, southern Labrador, demonstrates that skeletal carbonate was abundantly produced in these archaeocyathan reefs, although only about half was produced by archaeocyathans. Trilobites, echinoderms and brachiopods contributed substantially to the total carbonate budget, particularly in grainstone facies flanking the reefs. Through point count analysis of samples collected from the reef core and flanking grainstones, it can be demonstrated that skeletal material was most abundant in grainstone facies, where animals such as trilobites and echinoderms contributed significantly to carbonate production. In contrast, microbial fabrics were more abundant than skeletal fabrics in the reef core, although archaeocyathan material was more abundant than other skeletal debris. Similar to modern reefs, these reefs created a variety of habitats that allowed for the proliferation of skeletal organisms living on and around the reef, thereby promoting skeletal carbonate production through ecosystem engineering. □Archaeocyatha, bioherms, carbonates, calcification, point count analysis 相似文献
85.
Bees are known to collect pollen and nectar to provide their larvae and themselves with food. That bees, especially the tropical stingless bees (Apidae: Meliponini), also collect plant resins has, however, been barely addressed in scientific studies on resource use in bees. Resins are used for nest construction, nest maintenance and nest defence. Furthermore, some South‐East Asian species transfer resin‐derived terpenes to their cuticular profiles. The resin requirement of bees is in turn used by certain plant species, which attract bees either for pollination by providing resin in their inflorescences, or for seed dispersal by providing resin in their seed capsules (mellitochory). Mellitochory is found in the eucalypt tree Corymbia torelliana, the resin of which is collected by Australian stingless bees. We investigated how the interaction between C. torelliana and resin‐collecting bees affects the chemical ecology of two Australian stingless bee genera by comparing the chemical profiles of eight bee species with resin from C. torelliana fruits. The two bee genera differed significantly in their chemical profiles. Similar to South‐East Asian stingless bees, 51% of all compounds on the body surfaces of the five Tetragonula species were most likely derived from plant resins. Up to 32 compounds were identical with compounds from C. torelliana resin, suggesting that Tetragonula species include C. torelliana compounds in their chemical profiles. By contrast, few or none resinous compounds were found on the body surfaces of the three Austroplebeia species sampled. However, one prominent but as yet unknown substance was found in both C. torelliana resin and the chemical profiles of all Tetragonula and four Austroplebeia colonies sampled, suggesting that most colonies (76%) gathered resin from C. torelliana. Hence, C. torelliana resin may be commonly collected by Australian stingless bees and, along with resins from other plant species, shape their chemical ecology. 相似文献
86.
SARA H. THOMAS JESSICA M. HOUSLEY ABIGAIL N. REYNOLDS RACHEL M. PENCZYKOWSKI KATHRYN H. KENLINE NATALIE HARDEGREE SIERRA SCHMIDT MEGHAN A. DUFFY 《Freshwater Biology》2011,56(2):384-394
1. Recently, the potential for parasites to influence the ecology and evolution of their zooplankton hosts has been the subject of increasing study. However, most research to date has focussed on Daphnia hosts, and the potential for parasites to influence other zooplankton taxa remains largely unstudied. 2. During routine sampling of zooplankton in a eutrophic lake, we observed that the rotifer Asplanchna girodi was often infected with a parasitic oomycete. Epidemics of this parasite occurred frequently, with three separate events in a single year. Prevalence at peak infection ranged from 29 to 41% and epidemics lasted from 17 to 56 days. Our data indicate that high densities of the host population are required for epidemics to occur. 3. Our morphological and molecular analyses suggest that this parasite is in the genus Pythium. Most Pythium spp. are plant pathogens, but our study supports recent work on Daphnia, suggesting that Pythium spp. are also important parasites of zooplankton. 4. As the parasite in this study was recalcitrant to cultivation, we developed an alternative method to verify its identity. Our approach used quantitative PCR to show that the ribosomal sequences identified increased with increasing density of infected hosts and, thus, were associated with the parasite. This approach should be generally applicable to other plankton parasites that are difficult to cultivate outside their hosts. 5. Infections significantly reduced host fecundity, lifespan and population growth rate. As a result of the virulence of this parasite, it is likely to influence the population ecology and evolution of its Asplanchna host, and may be a useful model system for studies on host–parasite coevolutionary dynamics. 相似文献
87.
HERCULES MOURA SARA WALLACE GOVINDA S. VISVESVARA 《The Journal of eukaryotic microbiology》1992,39(5):573-583
ABSTRACT Two strains of Acanthamoeba isolated from human brain tissue and a strain of Acanthamoeba isolated from a fish were compared with 10 species of Acanthamoeba belonging to groups 1, 2 and 3 based on their isoenzyme profiles and antigenic characteristics. A total of 12 enzymes were studied. The isoenzymes and antigens were electrophoretically separated on polyacrylamide gradient gels, and the patterns obtained were compared after appropriate staining for particular enzymes and reactivities with homologous and heterologous rabbit anti- Acanthamoeba antisera. One of the human strains (CDC:1283:V013) was identified as A. healyi n. sp. because of its unique isoenzyme profiles for 11 of the 12 enzymes tested. The other human isolate was reidentified as A. culbertsoni because its isoenzyme profiles for 10 of 12 enzymes resembled those of A. culbertsoni , Lilly A-1 strain. Since the isoenzyme profiles and the antigenic patterns of the fish isolate as well were remarkably similar to those of A. royreba , it was considered as a strain of A. royreba . Polyacrylamide gradient gel electrophoresis appears to be a powerful technique for the study of isoenzymes and antigens of Acanthamoeba . 相似文献
88.
A Revised Medium for Growth of Pea Mesophyll Protoplasts 总被引:3,自引:0,他引:3
The nutrient requirements of mesophyll protoplasts from Pisum sativum L. cv. Timo have been investigated and a synthetic and completely defined medium has been designed. A high calcium concentration (12 mM) stimulated both protoplast survival and cell division. The content of iron and zinc was also critical. Additions of nicotinic acid, pyridoxine and thiamine were necessary. The protoplast growth was enhanced when some amino acids were included in the medium. An absolute requirement for auxin and cytokinin was shown. In the revised medium about 90% of the isolated protoplasts survived and formed a cell wall. The first divisions were observed after 5 days and after 1 week 10–20% of the cells had divided at least once. 相似文献
89.
90.
GIUSEPPINA PICHIRI MARIELLA NIEDDU SARA MANCONI CINZIA CASU PIERPAOLO CONI SUSANNA SALVADORI ROBERTO MEZZANOTTE 《Molecular ecology resources》2006,6(3):638-641
We cloned and sequenced the HaeIII 350‐bp 5S ribosomal DNA (rDNA) band of Anguilla rostrata and designed specific primers from this sequence. Polymerase chain reaction performed with these primers is able to distinguish DNA samples obtained from European (Anguilla anguilla) and American (Anguilla rostrata) eels. Two amplicons of 1200 bp and 600 bp were obtained, respectively, from A. rostrata and A. anguilla, and the whole 5S rDNA repeated unit from these eels was cloned and sequenced. Southern blot experiments, using four different restriction enzymes and the 5S nontranscribed spacers regions as probe, are able to point out specific diversity in these eels. 相似文献