Taxonomic Review of the Tribe Tenebrionini (Coleoptera, Tenebrionidae) in Korea

ABSTRACT The tribe Tenebrionini of Korean Tenebronidae is reviewed taxonomically. Five species, Encyalesthus violaceipennis (Marseul), Menephilus lucens Marseul, Neatus ventralis (Marseul), Tenebrio molitor Linné, Tenebrio obscurus Fabricius, are recognized in Korea. A key to diagnosis of the genera and species, and figures of these species are presented.     

CH4 production and oxidation processes in a boreal fen ecosystem after long‐term water table drawdown

Fens, which extend over vast areas in the Northern hemisphere, are sources of the greenhouse gas CH₄. Climate change scenarios predict a lowering water table (WT) in mires. To study the effect of WT drawdown on CH₄ dynamics in a fen ecosystem, we took advantage of a WT drawdown gradient near a ground water extraction plant. Methane fluxes and CH₄ production and oxidation potentials were related to microbial communities responsible for the processes in four mire locations (wet, semiwet, semidry, and dry). Principal component analyses performed on the vegetation, pH, CH₄, and WT results clearly separated the four sampling locations in the gradient. Long‐term lowering of WT was associated with decreased coverage of Sphagnum and aerenchymatic plants, decreased CH₄ field emissions and CH₄ production potential. Based on mcrA terminal restriction fragment length polymorphism the methanogen community structure correlated best with the methane production and coverage of aerenchymatic plants along the gradient. Methanosarcinaceae and Methanocellales were found at the pristine wet end of the gradient, whereas the Fen cluster characterized the dry end. The methane‐oxidizing bacterial community consisted exclusively of Methylocystis bacteria, but interestingly of five different alleles (T, S, R, M, and O) of the particulate methane monooxygenase marker gene pmoA. The M allele was dominant in the wet locations, and the occurrence of alleles O, S, and T increased with drainage. The occurrence of the R allele that characterized the upper peat layer correlated with CH₄ oxidation potential. These results advance our understanding of mire dynamics after long‐term WT drawdown and of the microbiological bases of methane emissions from mires.     

组蛋白去甲基化酶LSD1及其生物学功能

赖氨酸特异性组蛋白去甲基化酶1(Lysine specific demethylase 1, LSD1) 的发现, 表明组蛋白的甲基化修饰是一个动态可调节的过程。结构分析显示, LSD1 是一个黄素腺嘌呤二核苷酸(Flavin adenine dinulcleotide, FAD) 依赖性胺氧化酶, 它能够特异性脱去单甲基化和二甲基化组蛋白H3第4位赖氨酸(H3K4) 和H3K9 位点上的甲基基团。功能研究显示, LSD1 定位于细胞核内, 调控着基因转录的激活和抑制, 被誉为细胞深处的基因“开关”, 在胚胎发育和肿瘤发生过程中起着重要的作用。文章主要综述了LSD1 的结构、作用机制及其调控作用研究的新进展。     

光学显微镜下松柏类和菊科花粉的分类、鉴定要点及生态意义

本文对地层中广泛分布的松柏类和菊科花粉进行初步分类,讨论各类型(属)的鉴定特征及生态意义.以气囊的有无、外壁结构和纹饰、气囊的形态和纹饰、本体与气囊过渡点的特征、帽与帽缘纹理等为鉴定要点,对杉型、柏科型、刺柏型、红豆杉型、落叶松属、铁杉属、罗汉松属、云杉属、雪松属、冷杉属、油杉属和松属(双束松型和单束松型)13个类型的松柏类植物花粉逐步分类.通过总结前人研究和统计分析,讨论落叶松属、铁杉属、罗汉松属、云杉属、雪松属、冷杉属、油杉属和松属,特别是双束松型和单束松型花粉的生态环境指代意义.依据花粉的大小、外壁表面纹饰、外壁光切面中基柱层的结构和发育程度,初步拟将菊科花粉划分为6个类型,即菊苣-蒲公英型、紫菀型、春黄菊型、风毛菊型、蓝刺头型和蒿属,并通过对6个花粉类型植物母体生态环境的归纳总结和统计分析,讨论它们的生态环境指代意义.     

小鼠白细胞介素33真核表达质粒的构建及表达

克隆小鼠IL-33基因构建其真核表达质粒,并转染COS-7细胞检测其表达。提取C57BL/6小鼠肺组织总RNA,经反转录聚合酶链式反应（RT-PCR）扩增小鼠IL-33基因,酶切后插入pcDNA^TM3.1/myc HisA构建其真核表达质粒pcDNA-3.1-IL-33,重组质粒转染COS-7细胞,RT-PCR和免疫印迹法（western blotting）检测目的基因表达。结果显示,pcDNA3.1-IL-33中插入的片段序列测定结果与小鼠IL-33cDNA序列一致,重组质粒转染COS-7细胞后检测到相应mRNA及蛋白表达。成功克隆了小鼠IL-33基因cDNA,并构建其真核表达质粒。     

EFFECT OF MEAT APPEARANCE ON SOUTH KOREAN CONSUMERS' CHOICE OF PORK CHOPS DETERMINED BY IMAGE METHODOLOGY

The effect of appearance characteristics of fresh pork on Korean consumers' preferences was assessed using images of pork chops. Consumers (1015) in six provinces selected their preferred chop from 16 images modified systematically to give two levels each of fat cover, color, marbling and drip. Meat color was the most important characteristic; similar numbers of consumers chose the dark and light red meat colored pork. Almost two‐thirds of the consumers chose consistently the option without drip. A strong characteristic was that almost half of the Korean consumers chose the marbled pork, although fat cover was not an important selection criterion. Similar preferences were observed on a regional basis, except for color, which varied according to region. More female, particularly the married ones, than male consumers preferred the light red, no drip and marbled options.