Multiple Staining of Isopropanol Prepared Tissues with Phloxine B and Bismarck Brown Y

Two methods employing phioxine B and Bismarck brown Y followingdehydration and infiltration using a graded series of isopropylalcohol-paraffin combinations are presented: (1) a five-componentstain combination for highly differentiated plant tissues inwhich orange G is included to differentiate between the otherdyes and (2) a triple stain combination especially useful forgeneral differentiation. Both methods are uniformly successfuland result in colour-fast sections. Advantages of the isopropylalcohol technique are: diminished distortion and less hardeningof the material, ready availability, and low cost. Brief detailsof some of the stains used and their advantages are discussed. Phloxine B, organge G, Bismarck brown Y, isopropanol, Metasequoia glyptostroboides, dawn redwood, Polystichum tsus-Simense, Holly fern, histology     

The structure of the apical meristem of Isoetes engelmannii, I. riparia and I. macrospora (Isoetales)

The shoot apex in three species of Isoetes (I. engelmannii, I. macrospora and I. riparia ) is differentiated into a cap-like initiation layer of somewhat columnar meristematic cells which divide most frequently anticlinally and a subadjacent central core of cells which divide irregularly, but with periclinal divisions predominating. The conflicting views of apical organization in Isoetes are discussed.