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991.
To clarify the small-scale heterogeneity of light regimes in a rain forest, photosynthetic photon flux density (PFD) was measured at 1-min intervals during six days at 12 microsites in each of two plots, a small gap and an understory in Pasoh Forest Reserve, Peninsular Malaysia. Frequency distribution of microsite PFD was unimodal with the peak value between 16 and 32 μmol/m2/sec in the small gap, but between 8 and 16 μmol/m2/sec in the understory. In the small gap, PFD was more variable among microsites; total daily PFD and daily sunfleck PFD exceeding 10 μmol/ m2/sec tended to be higher (P <0.05; t-test) compared to those in the understory. Sunfleck PFD exceeding 50 μmol/ m2/sec, however, showed no difference between the two plots. Diffuse PFD transmittance, defined as the ratio of PFD in the forest to that measured at 43 m above ground during the periods 0800-0810 and 1750-1800 h, was significantly higher in the small gap than in the understory plot. Diffuse PFD transmittance was also positively correlated with microsite total daily PFD. To examine the effects of the subtle heterogeneity of light regimes on leaf carbon gain, we simulated carbon gain by sun and shade leaves in a typical shade-tolerant species, Brosimum aticastrum Sw. (Moraceae). Despite the similarity in total daily PFD, total daily carbon gain was considerably higher in the gap than in the understory for both sun and shade leaves. This study suggests that frequency distribution of PFD is critical in describing microsite PFD regimes and determining leaf carbon gain in the tropical forest floor. 相似文献
992.
Fujita Yoshihiko; Murakami Akio; Ohki Kaori; Hagiwara Naoki 《Plant & cell physiology》1988,29(4):557-564
The quantitative composition of thylakoid components such asthe photosystem (PS) I/PS II ratio in the cyanobacterial photosyntheticsystem is regulated in response to the light regime. The regulationoccurs as changes in PS I content due to control of either PSI formation or decomposition. In order to determine which ofthese two is controlled in this regulation, experiments wereperformed to determine the light-induced PS I decrease in cellsof Synechocystis PCC 6714 under conditions where protein synthesiswas suppressed, i.e. the incubation without a nitrogen sourcefor cell growth or with chloramphenicol. The results revealedthat light-induced PS I decrease did not occur when synthesisof the thylakoid system was suppressed by incubation withouta nitrogen source or by the addition of chloramphenicol, indicatingthat (1) the thylakoid composition is regulted in the processof thylakoid formation and (2) the regulation is achieved bythe control of PS I formation. (Received November 6, 1987; Accepted March 2, 1988) 相似文献
993.
Yojiro Anzai Yohei Iizaka Wei Li Naoki Idemoto Shu-ichi Tsukada Kazuo Koike Kenji Kinoshita Fumio Kato 《Journal of industrial microbiology & biotechnology》2009,36(8):1013-1021
Some of the polyketide-derived bioactive compounds contain sugars attached to the aglycone core, and these sugars often impart
specific biological activity to the molecule or enhance this activity. Mycinamicin II, a 16-member macrolide antibiotic produced
by Micromonospora griseorubida A11725, contains a branched lactone and two different deoxyhexose sugars, d-desosamine and d-mycinose, at the C-5 and C-21 positions, respectively. The d-mycinose biosynthesis genes, mycCI, mycCII, mycD, mycE, mycF, mydH, and mydI, present in the M. griseorubida A11725 chromosome were introduced into pSET152 under the regulation of the promoter of the apramycin-resistance gene aac(3)IV. The resulting plasmid pSETmycinose was introduced into Micromonospora rosaria IFO13697 cells, which produce the 16-membered macrolide antibiotic rosamicin containing a branched lactone and d-desosamine at the C-5 position. Although the M. rosaria TPMA0001 transconjugant exhibited low rosamicin productivity, two new compounds, IZI and IZII, were detected in the ethylacetate
extract from the culture broth. IZI was identified as a mycinosyl rosamicin derivative, 23-O-mycinosyl-20-deoxo-20-dihydro-12,13-deepoxyrosamicin (MW 741), which has previously been synthesized by a bioconversion technique.
This is the first report on production of mycinosyl rosamicin-derivatives by a engineered biosynthesis approach. The integration
site ΦC31attB was identified on M. rosaria IFO13697 chromosome, and the site lay within an ORF coding a pirin homolog protein. The pSETmycinose could be useful for
stimulating the production of “unnatural” natural mycinosyl compounds by various actinomycete strains using the bacteriophage
ΦC31 att/int system. 相似文献
994.
Biodegradation of diphenylarsinic acid to arsenic acid by novel soil bacteria isolated from contaminated soil 总被引:2,自引:0,他引:2
Naoki Harada Kazuhiro Takagi Koji Baba Kunihiko Fujii Akio Iwasaki 《Biodegradation》2010,21(3):491-499
Microorganisms capable of degrading diphenylarsinic acid (DPAA) were enriched from contaminated soil using the soil-charcoal
perfusion method. Two novel bacterial strains, L2406 and L2413, that can degrade DPAA in a mineral salt medium supplemented
with DPAA as the sole carbon source were isolated. Based on comparative morphology, physiology, and comparison of the 16S
rRNA gene sequences, both were presumed to be species closely related to Ensifer adhaerens. As the metabolites, phenylarsonic acid (PAA) was determined by liquid chromatography-mass spectrometry analysis as well
as three unknown peaks all of whose molecular weights were estimated to be 278. The increase of m/z = 16 from DPAA in the
unknowns suggests monohydroxylation of DPAA at the 2-, 3- and 4-positions. The ability of strains L2406 and L2413 to degrade
DPAA was suppressed in iron insufficient conditions, e.g. less than 7.2 μM iron in the culture medium. These facts strongly
suggest the following hypothesis: Monooxygenase works at the initial degradation step of DPAA degradation by the isolates;
and direct hydrolysis from DPAA to PAA is not likely to occur. In addition, release of arsenic acid from PAA by strain L2406
was confirmed by liquid chromatography-inductively coupled plasma mass spectrometry. From these results, strain L2406 was
considered to be capable of degrading DPAA to arsenic acid via PAA when DPAA was supplied as the sole carbon source. 相似文献
995.
Yoshida N Nakasato M Ohmura N Ando A Saiki H Ishii M Igarashi Y 《Current microbiology》2006,53(5):406-411
A novel thermoacidophilic iron-reducing Archaeon, strain NA−1, was isolated from a hot fumarole in Manza, Japan. Strain NA-1 could grow autotrophically using H2 or S0 as an electron donor and Fe3+ as an electron acceptor, and also could grow heterotrophically using some organic compounds. Fe3+ and O2 served as electron acceptors for growth. However, S0, NO3
−, NO2
−, SO4
2−, Mn4+, fumarate, and Fe2O3 did not serve as electron acceptors. The ranges of growth temperature and pH were 60–90°C (optimum: 80°C) and pH 1.0–5.0
(optimum: pH 1.2–1.5), respectively. Cells were nearly regular cocci with an envelope comprised of the cytoplasmic membrane
and a single outer S-layer. The crenarchaeal-specific quinone (cardariellaquinone) was detected, and the genomic DNA G + C
content was 29.9 mol%. From 16S rDNA analysis, it was determined that strain NA-1 is closely related to Acidianus ambivalens (93.1%) and Acidianus infernus (93.0%). However, differences revealed by phylogenetic and phenotypic analyses clearly show that strain NA-1 represents a
new species, Acidianus manzaensis, sp. nov., making it the first identified thermoacidophilic iron-reducing microorganism (strain NA-1T = NBRC 100595 = ATCC BAA 1057).
Strain NA-1 has been deposited in the culture collections of the National Institute of Technology and Evolution (NBRC 100595)
and American Type Culture Collection (ATCC BAA 1057). The 16S rDNA sequence has been deposited at GenBank under accession
number AB182498. 相似文献
996.
ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation 总被引:1,自引:0,他引:1
Takase T Nakazawa M Ishikawa A Kawashima M Ichikawa T Takahashi N Shimada H Manabe K Matsui M 《The Plant journal : for cell and molecular biology》2004,37(4):471-483
To study the GH3 gene family of Arabidopsis, we investigated a flanking sequence database of Arabidopsis activation-tagged lines. We found a dwarf mutant, named yadokari 1-D (ydk1-D), that had a T-DNA insertion proximal to a GH3 gene. ydk1-D is dominant and has a short hypocotyl not only in light but also in darkness. Moreover, ydk1-D has a short primary root, a reduced lateral root number, and reduced apical dominance. A GH3 gene, named YDK1, was upregulated in ydk1-D, and YDK1 transgenic plants showed the ydk1-D phenotype. YDK1 gene expression was induced by exogenously applied auxin and regulated by auxin-response factor (ARF)7. In addition, YDK1 gene expression was downregulated by blue and far-red (FR) lights. Strong promoter activity of YDK1 was observed in roots and flowers. These results suggest that YDK1 may function as a negative component in auxin signaling by regulating auxin activity. 相似文献
997.
Apoptosis induced by the histone deacetylase inhibitor FR901228 in human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells 总被引:7,自引:0,他引:7 下载免费PDF全文
Mori N Matsuda T Tadano M Kinjo T Yamada Y Tsukasaki K Ikeda S Yamasaki Y Tanaka Y Ohta T Iwamasa T Tomonaga M Yamamoto N 《Journal of virology》2004,78(9):4582-4590
Inhibition of histone deacetylase (HDAC) activity induces growth arrest, differentiation, and, in certain cell types, apoptosis. FR901228, FK228, or depsipeptide, is an HDAC inhibitor effective in T-cell lymphomas. Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1) and remains incurable. We examined whether FR901228 is effective for treatment of ATL by assessing its ability to induce apoptosis of HTLV-1-infected T-cell lines and primary leukemic cells from ATL patients. FR901228 induced apoptosis of Tax-expressing and -unexpressing HTLV-1-infected T-cell lines and selective apoptosis of primary ATL cells, especially those of patients with acute ATL. FR901228 also efficiently reduced the DNA binding of NF-kappaB and AP-1 in HTLV-1-infected T-cell lines and primary ATL cells and down-regulated the expression of Bcl-x(L) and cyclin D2, regulated by NF-kappaB. Although the viral protein Tax is an activator of NF-kappaB and AP-1, FR901228-induced apoptosis was not associated with reduced expression of Tax. In vivo use of FR901228 partly inhibited the growth of tumors of HTLV-1-infected T cells transplanted subcutaneously in SCID mice. Our results indicated that FR901228 could induce apoptosis of these cells and suppress the expression of NF-kappaB and AP-1 and suggest that FR901228 could be therapeutically effective in ATL. 相似文献
998.
Naoki Nishizawa Taiji Asami Kimiko Nishibori Yoshihiro Takatsu Atsuko Suzuki Kazutaka Ushio Shin-ichi Matsumoto Yuji Shimizu Masashi Yamaguchi Masami Kusaka Hisanori Matsui Tetsuya Ohtaki Chieko Kitada 《Bioorganic & medicinal chemistry letters》2019,29(4):654-658
The kisspeptin (Kp, Kp-54, metastin)/KISS1R system plays crucial roles in regulating the secretion of gonadotropin-releasing hormone. Continuous administration of nonapeptide Kp analogs caused plasma testosterone depletion, whereas bolus administration caused strong plasma testosterone elevation in male rats. To develop a new class of small peptide drugs, we focused on stepwise N-terminal truncation of Kp analogs and discovered potent pentapeptide analogs. Benzoyl-Phe-azaGly-Leu-Arg(Me)-Trp-NH2 (16) exhibited high agonist activity for KISS1R and excellent metabolic stability in rat serum. A single injection of a 4-pyridyl analog (19) at the N-terminus of 16 into male Sprague Dawley rats caused a robust increase in plasma luteinizing hormone levels, but unlike continuous administration of nonapeptide Kp analogs, continuous administration of 19 maintained moderate testosterone levels in rats. These results indicated that small peptide drugs can be successfully developed for treating sex hormone deficiency. 相似文献
999.
Formation and carbon monoxide‐dependent dissociation of Allochromatium vinosum cytochrome c′ oligomers using domain‐swapped dimers 下载免费PDF全文
Masaru Yamanaka Makoto Hoshizumi Satoshi Nagao Ryoko Nakayama Naoki Shibata Yoshiki Higuchi Shun Hirota 《Protein science : a publication of the Protein Society》2017,26(3):464-474
The number of artificial protein supramolecules has been increasing; however, control of protein oligomer formation remains challenging. Cytochrome c′ from Allochromatium vinosum (AVCP) is a homodimeric protein in its native form, where its protomer exhibits a four‐helix bundle structure containing a covalently bound five‐coordinate heme as a gas binding site. AVCP exhibits a unique reversible dimer–monomer transition according to the absence and presence of CO. Herein, domain‐swapped dimeric AVCP was constructed and utilized to form a tetramer and high‐order oligomers. The X‐ray crystal structure of oxidized tetrameric AVCP consisted of two monomer subunits and one domain‐swapped dimer subunit, which exchanged the region containing helices αA and αB between protomers. The active site structures of the domain‐swapped dimer subunit and monomer subunits in the tetramer were similar to those of the monomer subunits in the native dimer. The subunit–subunit interactions at the interfaces of the domain‐swapped dimer and monomer subunits in the tetramer were also similar to the subunit–subunit interaction in the native dimer. Reduced tetrameric AVCP dissociated to a domain‐swapped dimer and two monomers upon CO binding. Without monomers, the domain‐swapped dimers formed tetramers, hexamers, and higher‐order oligomers in the absence of CO, whereas the oligomers dissociated to domain‐swapped dimers in the presence of CO, demonstrating that the domain‐swapped dimer maintains the CO‐induced subunit dissociation behavior of native ACVP. These results suggest that protein oligomer formation may be controlled by utilizing domain swapping for a dimer–monomer transition protein. 相似文献
1000.
Iigaya K Kumagai H Onimaru H Kawai A Oshima N Onami T Takimoto C Kamayachi T Hayashi K Saruta T Itoh H 《American journal of physiology. Regulatory, integrative and comparative physiology》2007,292(2):R927-R936
We used an optical imaging technique to investigate whether axons of neurons in the caudal end of the ventrolateral medulla (CeVLM), as well as axons of neurons in the rostral ventrolateral medulla (RVLM), project to neurons in the intermediolateral cell column (IML) of the spinal cord. Brain stem-spinal cord preparations from neonatal normotensive Wistar-Kyoto and spontaneously hypertensive rats were stained with a voltage-sensitive dye, and responses to electrical stimulation of the IML at the Th2 level were detected as changes in fluorescence intensity with an optical imaging apparatus (MiCAM-01). The results were as follows: 1) depolarizing responses to IML stimulation during low-Ca high-Mg superfusion were detected on the ventral surface of the medulla at the level of the CeVLM, as well as at the level of the RVLM, 2) depolarizing responses were also detected on cross sections at the level of the CeVLM, and they had a latency of 24.0 +/- 5.5 (SD) ms, 3) antidromic action potentials in response to IML stimulation were demonstrated in the CeVLM neurons where optical images were detected, and 4) glutamate application to the CeVLM increased the frequency of excitatory postsynaptic potentials (EPSPs) and induced depolarization of the IML neurons. The optical imaging findings suggested a novel axonal and functional projection from neurons in the CeVLM to the IML. The increase in EPSPs of the IML neurons in response to glutamate application suggests that the CeVLM participates in the regulation of sympathetic nerve activity and blood pressure and may correspond to the caudal pressor area. 相似文献