全文获取类型
收费全文 | 934691篇 |
免费 | 101685篇 |
国内免费 | 682篇 |
专业分类
1037058篇 |
出版年
2018年 | 8813篇 |
2017年 | 8477篇 |
2016年 | 11746篇 |
2015年 | 15058篇 |
2014年 | 18069篇 |
2013年 | 26222篇 |
2012年 | 28917篇 |
2011年 | 29852篇 |
2010年 | 20141篇 |
2009年 | 18368篇 |
2008年 | 26154篇 |
2007年 | 27124篇 |
2006年 | 25397篇 |
2005年 | 24558篇 |
2004年 | 24205篇 |
2003年 | 23382篇 |
2002年 | 22706篇 |
2001年 | 44099篇 |
2000年 | 44137篇 |
1999年 | 34677篇 |
1998年 | 11469篇 |
1997年 | 12054篇 |
1996年 | 11365篇 |
1995年 | 10628篇 |
1994年 | 10321篇 |
1993年 | 10280篇 |
1992年 | 28312篇 |
1991年 | 27252篇 |
1990年 | 26683篇 |
1989年 | 26053篇 |
1988年 | 24102篇 |
1987年 | 22773篇 |
1986年 | 21018篇 |
1985年 | 21061篇 |
1984年 | 17301篇 |
1983年 | 14852篇 |
1982年 | 11198篇 |
1981年 | 10109篇 |
1980年 | 9514篇 |
1979年 | 16514篇 |
1978年 | 12699篇 |
1977年 | 11745篇 |
1976年 | 10968篇 |
1975年 | 12104篇 |
1974年 | 13025篇 |
1973年 | 12979篇 |
1972年 | 11770篇 |
1971年 | 10810篇 |
1970年 | 9370篇 |
1969年 | 8947篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
951.
A total of 764 breeding soundness examinations was conducted on beef bulls utilizing the method of examination and criteria for classifying bulls of the Society for Theriogenology. In addition to this examination each bull was subjected to scrotal circumference measurement and to weighing. Classification of the bulls according to breeding soundness potentials was as follows: 88% of the bulls were satisfactory potential breeders, 8% of the bulls were questionable potential breeders and 4% of the bulls were unsatisfactory potential breeders. The proportions of bulls in each classification; satisfactory, questionable, or unsatisfactory; were not different among the four breeds evaluated. The majority of bulls evaluated in this study were between 14 and 36 months of age and weighed between 900 and 1500 pounds. For the ages and weights evaluated, scrotal circumference measurement variances were not closely related to age and weight differences. There was a tendency shown for “Questionable” and “Unsatisfactory Potential Breeders” to have smaller scrotal circumference measurements. The study indicates that Angus, Charolais, Horned Hereford and Polled Hereford bulls of breeding ages and weights should have scrotal circumference measurements of at least 32 centimeters in order to be classified as “Satisfactory Potential Breeders”. 相似文献
952.
953.
B Hollenbach E Scherzinger K Schweiger R Lurz H Lehrach E E Wanker 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1999,354(1386):991-994
We have shown previously by electron microscopy that the purified glutathione S-transferase (GST)-Huntington's disease (HD) exon 1 fusion protein with 51 glutamine residues (GST-HD51) is an oligomer, and that site-specific proteolytic cleavage of this fusion protein results in the formation of insoluble more highly ordered protein aggregates with a fibrillar or ribbon-like morphology (E. Scherzinger et al. (1997) Cell 90, 549-558). Here we report that a truncated GST HD exon 1 fusion protein with 51 glutamine residues, which lacks the proline-rich region C-terminal to the polyglutamine (polyQ) tract (GST-HD51 delta P) self-aggregates into high-molecular-mass protein aggregates without prior proteolytic cleavage. Electron micrographs of these protein aggregates revealed thread-like fibrils with a uniform diameter of ca. 25 nm. In contrast, proteolytic cleavage of GST-HD51 delta P resulted in the formation of numerous clusters of high-molecular-mass fibrils with a different, ribbon-like morphology. These structures were reminiscent of prion rods and beta-amyloid fibrils in Alzheimer's disease. In agreement with our previous results with full-length GST-HD exon 1, the truncated fusion proteins GST-HD20 delta P and GST-HD30 delta P did not show any tendency to form more highly ordered structures, either with or without protease treatment. 相似文献
954.
Utilization of mangrove wood products around mida creek (Kenya) amongst subsistence and commercial users 总被引:1,自引:0,他引:1
Mida Creek (Kenya) comprises mangrove forests and other marine resources that are of economic, ecological, and environmental importance to the local village communities. In total 116 households (100 of which could be used for numerical analysis), which are estimated to correspond to a coverage of ca. 30% of the total Mida Creek population, were interviewed to assess the human reliance on mangrove resources in Mida Creek. The survey indicates that mangroves are a major resource of wood for house construction, fuel wood, charcoal, and boat building. Minor uses of mangrove products include pharmaceutical and medicinal applications, tanning material, and furniture making. Rhizophora mucronata, Ceriops tagal, and Bruguiera gymnorrhiza are the major resources for house construction and fuel wood, while Sonneratia alba and Xylocarpus granatum were reported to be useful for boat building and medicinal uses respectively. The survey further describes harvesting activities and house construction, and reveals species preferences within this one particular use. As a result of depletion of the supply and the banning of mangrove harvesting, the local people are turning to other wood materials and to poaching. In our view, local utilization patterns rather than global usefulness data are required to establish a conservation policy of both mangroves and users’ subsistence requirements. 相似文献
955.
Spatial, temporal and habitat-related variation in the abundance of large predatory fish at One Tree Reef, Australia 总被引:1,自引:1,他引:0
Patterns of abundance of large piscivorous fish (>200 mm TL) were documented at two spatial and four temporal scales within
the main lagoon of One Tree Reef on Australia’s Great Barrier Reef. Grouper (Serranidae), snapper (Lutjanidae) and wrasses
(Labridae) were the most abundant large piscivores. On a large scale (hundreds of metres), patterns of predator abundance
were consistently greater on the inner edge than centre of the lagoon over a range of temporal scales: days, weeks, months
and years. On a small spatial scale (tens of metres), the abundance of large predatory fish was patchy. At both spatial scales,
fish were consistently aggregated in particular areas and associated with specific structural features of the reef habitat.
Predator abundance was high where live corals were predominant and the topography was more complex. Hence, predation pressure
and its potential effects on the distribution and abundance of prey populations, both in time and space, may vary greatly
within lagoonal environments.
Accepted: 25 May 1997 相似文献
956.
957.
958.
Recent evidence suggests that the nutritional state of male Mediterranean fruit flies, Ceratitis capitata (Wied.) (medfly), is an important influence on various components of their reproductive biology, including mating success. The objective of the present study was to examine experimentally the effect of temporary starvation on the mating success of wild male C. capitata. Males were maintained on protein–sugar or sugar-only diets, and for each diet we compared the mating success of continuously fed males versus males starved for 18 or 24 h immediately before testing. In trials conducted on field-caged, host trees, males starved for 24 h obtained only about half as many matings as fed males for both diets. However, when the starvation period was 18 h, starved males reared on the protein–sugar diet mated significantly less frequently than fed males, whereas starved males reared on sugar mated as often as fed males. Measurements of male pheromone calling and female attraction revealed that reduced mating success likely reflected the decreased signaling activity of starved males. 相似文献
959.
Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. 下载免费PDF全文
We have taxonomically and phylogenetically characterized a new aerobic bacterial strain (JF-1) that contains photosynthetic pigment-protein complexes and which was recently isolated from black smoker plume waters of the Juan de Fuca Ridge. Strain JF-1 is a gram-negative, yellow-pigmented, motile bacterium that is salt-, pH-, and thermotolerant. These properties are consistent with an oligotrophic adaptation to varied environmental conditions thought to exist around deep-sea hydrothermal vents. The analysis of 16S rDNA sequences revealed that strain JF-1 forms a separate phylogenetic branch between the genus Erythromonas and the Erythromicrobium-Porphyrobacter-Erythrobacter cluster within the alpha subclass of the Proteobacteria. The taxonomic name Citromicrobium bathyomarinum (gen. nov., sp. nov.) is proposed for strain JF-1. 相似文献
960.
Ruth C. Paul B. Rainey Brian J. Sheehan Orla M. Keane Charles J. Dorman 《Current biology : CB》1999,9(24)
The relationship between environment and mutation is complex [1]. Claims of Lamarkian mutation [2] have proved unfounded [3], [4] and [5]; it is apparent, however, that the external environment can influence the generation of heritable variation, through either direct effects on DNA sequence [6] or DNA maintenance and copying mechanisms [7], [8], [9] and [10], or as a consequence of evolutionary processes [11], [12], [13], [14], [15] and [16]. The spectrum of mutational events subject to environmental influence is unknown [6] and precisely how environmental signals modulate mutation is unclear. Evidence from bacteria suggests that a transient recombination-dependent hypermutational state can be induced by starvation [5]. It is also apparent that chnages in the mutability of specific loci can be influenced by alterations in DNA topology [10] and [17]. Here we describe a remarkable instance of adaptive evolution in Salmonella which is caused by a mutation that occurs in intermediate-strength osmotic environments. We show that the mutation is not ‘directed’ and describe its genetic basis. We also present compelling evidence in support of the hypothesis that the mutational event is constrained by signals transmitted from the external environment via changes in the activity of DNA gyrase. 相似文献