全文获取类型
收费全文 | 280篇 |
免费 | 12篇 |
出版年
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 7篇 |
2014年 | 6篇 |
2013年 | 28篇 |
2012年 | 7篇 |
2011年 | 19篇 |
2010年 | 7篇 |
2009年 | 8篇 |
2008年 | 14篇 |
2007年 | 8篇 |
2006年 | 25篇 |
2005年 | 21篇 |
2004年 | 18篇 |
2003年 | 18篇 |
2002年 | 18篇 |
2001年 | 5篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 5篇 |
1993年 | 4篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1974年 | 1篇 |
1971年 | 2篇 |
排序方式: 共有292条查询结果,搜索用时 31 毫秒
51.
Previous physiological studies have suggested central roles of brain natriuretic peptide (BNP). However, little information is available about the localization of BNP in the brain. In this study, we determined cDNA sequence encoding the entire coding region of prepro-BNP of Japanese and cynomologus monkeys, and then examined the immunohistochemical localization of BNP in the monkey hypothalamus. Japanese and cynomologus monkey prepro-BNP consisted of 132 amino acid residues with biologically active C-terminal 32 amino acids. Comparisons of deduced amino acid sequences among different species revealed high homology between monkey and human (91% in prerpro-BNP and 97% in the mature region). Immunohistochemical examination showed that BNP immunoreactive dots were observed in the paraventricular, periventricular, and supraoptic nuclei of the monkey hypothalamus. The present result suggests the central role of BNP in the neuroendocrine system in the hypothalamus. 相似文献
52.
53.
In vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells 总被引:2,自引:0,他引:2
Abdelalim EM Takada T Toyoda F Omatsu-Kanbe M Matsuura H Tooyama I Torii R 《Biochemical and biophysical research communications》2006,340(2):689-695
Functional characterization of ES cell-derived cardiomyocytes is important for differentiation control and application to the cell therapy. One of the crucial functions of cardiomyocytes is a production of atrial and brain natriuretic peptides (ANP and BNP, respectively), which have important endocrine, autocrine, and paracrine functions. In this study, we focused on the functional aspect of the cardiomyocytes differentiated from monkey ES cells in vitro and investigated the expression of ANP and BNP. Spontaneously contracting cells showed nodal-like action potentials, and expression of ANP and BNP by RT-PCR and immunocytochemistry. Interestingly, ANP and BNP expressions were detected as immunoreactive granules in the perinuclear area and these signals appeared to co-localize with trans-Golgi network. These findings suggest that monkey ES cells were able to differentiate into cardiomyocytes with functional characteristics in vitro and therefore can be used as a useful model to study mechanisms and functions in early cardiogenesis. 相似文献
54.
55.
Masuda T Inouchi T Fujimoto A Shingai Y Inai M Nakamura M Imai S 《Bioscience, biotechnology, and biochemistry》2012,76(4):705-711
The functionality of spring mountain herbs, which were collected in the Kajigamori mountain area of Shikoku area in Japan, was investigated in the course of our studies for utilizing local plant resources. The radical scavenging activity of the extracts from seventeen herbs was measured. Among these herbs, two extracts from Polystichym ovato-paleaceum (Japanese name: Tsuyanashiinode) and Sambucus racemosa subsp. sieboldiana (Japanese name: Niwatoko) showed potent DPPH radical scavenging activity. The material evidence for the potent activity of the extracts was studied by a combination of our developed method for detecting antiradical compounds, LC-MS/MS, and enzymatic hydrolysis. 相似文献
56.
57.
Andoh T Matsubara T Harumi T Yanagimachi R 《The International journal of developmental biology》2008,52(5-6):753-757
The fish egg is surrounded by a thick envelope called the chorion. The fertilizing spermatozoon enters the egg through a canal-like structure in the chorion, the micropyle. Examination of micropyle at fertilization is difficult if eggs are large and have no distinct landmarks surrounding the micropyle, or if they are positively buoyant in water. Eggs of many commercially important fishes (e.g., flounder, sea bream and eel) are buoyant in water or only slightly adhere to solid objects (e.g., sands, rock and water plants), which makes observation of spermatozoa at fertilization difficult. Here, we report that such eggs can be firmly attached to plastic and glass dishes that have been previously coated with poly-L-lysine. These adhering eggs can be fertilized and develop normally on the dishes. Observations of micropyles of three fish species, before and after sperm entry are presented. 相似文献
58.
59.
In the central nervous system, astrocytes produce erythropoietin (Epo) and neurons express its receptor. To examine whether
or not the brain Epo protects the in vitro cultured neurons from glutamate-induced cell death, we established rat astrocyte
cell lines containing the plasmid for production of recombinant rat Epo. Epo partially purified from the culture medium showed
a neuroprotective effect similar to that of rat Epo produced by Chinese hamster ovary (CHO) cells. Comparison was made in
some other properties between Epo produced by these astrocyte cell lines and that by CHO cells. Digestion of Epo with glycosidases
indicated that there was a little difference in glycosylation of Epo produced by two types of the cells.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
60.
Hiromi Takaki Hiroyuki Oshiumi Masashi Shingai Misako Matsumoto Tsukasa Seya 《Microbiology and immunology》2017,61(3-4):107-113
Viruses usually exhibit strict species‐specificity as a result of co‐evolution with the host. Thus, in mouse models, a great barrier exists for analysis of infections with human‐tropic viruses. Mouse models are unlikely to faithfully reproduce the human immune response to viruses or viral compounds and it is difficult to evaluate human therapeutic efficacy with antiviral reagents in mouse models. Humans and mice essentially have different immune systems, which makes it difficult to extrapolate mouse results to humans. In addition, apart from immunological reasons, viruses causing human diseases do not always infect mice because of species tropism. One way to determine tropism would be a virus receptor that is expressed on affected cells. The development of gene‐disrupted mice and Tg mice, which express human receptor genes, enables us to analyze several viral infections in mice. Mice are, indeed, susceptible to human viruses when artificially infected in receptor‐supplemented mice. Although the mouse cells less efficiently permit viral replication than do human cells, the models for analysis of human viruses have been established in vivo as well as in vitro, and explain viral pathogenesis in the mouse systems. In most systems, however, nucleic acid sensors and type I interferon suppress viral propagation to block the appearance of infectious manifestation. We herein review recent insight into in vivo antiviral responses induced in mouse infection models for typical human viruses. 相似文献