全文获取类型
收费全文 | 1744篇 |
免费 | 103篇 |
出版年
2021年 | 7篇 |
2020年 | 9篇 |
2019年 | 10篇 |
2018年 | 15篇 |
2017年 | 13篇 |
2016年 | 22篇 |
2015年 | 41篇 |
2014年 | 42篇 |
2013年 | 87篇 |
2012年 | 90篇 |
2011年 | 93篇 |
2010年 | 56篇 |
2009年 | 56篇 |
2008年 | 76篇 |
2007年 | 98篇 |
2006年 | 84篇 |
2005年 | 89篇 |
2004年 | 89篇 |
2003年 | 69篇 |
2002年 | 65篇 |
2001年 | 67篇 |
2000年 | 70篇 |
1999年 | 56篇 |
1998年 | 23篇 |
1997年 | 27篇 |
1996年 | 22篇 |
1995年 | 28篇 |
1994年 | 23篇 |
1993年 | 18篇 |
1992年 | 37篇 |
1991年 | 44篇 |
1990年 | 34篇 |
1989年 | 33篇 |
1988年 | 26篇 |
1987年 | 27篇 |
1986年 | 23篇 |
1985年 | 22篇 |
1984年 | 25篇 |
1983年 | 16篇 |
1982年 | 11篇 |
1981年 | 11篇 |
1980年 | 8篇 |
1979年 | 11篇 |
1978年 | 9篇 |
1977年 | 7篇 |
1975年 | 11篇 |
1972年 | 5篇 |
1970年 | 5篇 |
1969年 | 8篇 |
1967年 | 4篇 |
排序方式: 共有1847条查询结果,搜索用时 703 毫秒
21.
A major plasma protein, referred to as SP 1, exhibits sex- and stage-specific expression during the larval development of the silkworm, Bombyx mori. We have isolated a plasmid clone bearing a part of mRNA sequence coding for SP 1 and quantitated the amount of SP 1 mRNA by means of RNA blot hybridization using the cDNA probe. The developmental change in the amount of SP 1 mRNA in the fat body closely reflected that of the hemolymph concentration of SP 1, indicating that the biosynthesis of SP 1 is regulated in a sex- and developmental stage-specific fashion at the level of mRNA. 相似文献
22.
Fujioka Shozo; Sakurai Akira; Yamaguchi Isomaro; Murofushi Noboru; Takahashi Nobutaka; Kaihara Sumiko; Takimoto Atsushi; Cleland Charles F. 《Plant & cell physiology》1986,27(7):1297-1307
The occurrence and endogenous level of various plant hormoneswere measured for the short-day plants Lemna paucicostata 151and 381 and the long-day plant Lemna gibba G3 to determine whetherany of them are involved in the photoperiodic control of flowering.ABA, IAA, GA1, GA29, GA34, GA53, trans- and cis-zeatin, trans-and cis-ribosyl zeatin, N6-(2-isopentenyl) adenine and N6-(2-isopentenyl)adenosine were definitely detected in each species, while GA4was only detected in L. gibba G3 and GA20 was only detectedin L. paucicostata 151. The endogenous levels of ABA and IAAwere in the range of 17 ng/g fr wt and were not significantlydifferent in vegetative and flowering plants. The endogenousgibberellin levels were generally higher in Lemna grown underlong-day rather than short-day conditions. The endogenous cytokininlevels were almost the same in both flowering and vegetativeplants of L. paucicostata 151 and 381. In L. gibba G3, however,the level of cis-ribosyl zeatin, N6-(2-isopentenyl) adenineand N6-(2-sopentenyl) adenosine were higher in vegetative thanin flowering plants. These results indicate that there is not necessarily a directrelation between endogenous plant hormone levels and flowering,and that the chemical basis for the photoperiodic control offlowering cannot be explained solely by changes in hormone levels.The possibility remains, however, that one or more of the planthormones has some influence of secondary importance on the floweringprocess in Lemna. (Received January 29, 1986; Accepted July 12, 1986) 相似文献
23.
Type IV collagen is a major structural component in basement membranes. It is considerably different from the fibrillar collagens, types I-III. For example, unlike fibrillar collagens, the triple helical domain of type IV collagen is frequently interrupted by nonhelical regions. In this report, we demonstrate several overlapping genomic clones which cover most of the mouse alpha 1(IV) chain. Electron microscopic analysis of R-loops revealed that there were at least 28 exons within 35 kilobases of the gene segment. The sizes of six exons were determined by DNA sequence analysis to be 81, 178, 134, 73, 129, and 213 base pairs. These sizes do not appear to be related to the 54-base pair coding unit which is characteristic of fibrillar collagen exons, suggesting that the alpha 1 type IV collagen gene evolved differently from the fibrillar collagen genes. 相似文献
24.
Atrial natriuretic factor inhibits vasopressin secretion from rat posterior pituitary 总被引:4,自引:0,他引:4
K Obana M Naruse T Inagami A B Brown K Naruse F Kurimoto H Sakurai H Demura K Shizume 《Biochemical and biophysical research communications》1985,132(3):1088-1094
The effects of synthetic atrial natriuretic factor (ANF) were studied in superfused rat posterior pituitary gland. ANF (10(-6)M, 10(-10)M) significantly inhibited basal as well as KC1 (50 mM) or angiotensin II-stimulated immunoreactive arginine vasopressin secretion. The magnitude of inhibition was greater at 10(-6)M than at 10(-10)M. ANF also decreased cAMP secretion and increased cGMP secretion from the posterior pituitary. These results suggest that ANF directly acts on the posterior pituitary to inhibit arginine vasopressin secretion and that this effect is, at least, partly mediated by the changes in cyclic nucleotide production. 相似文献
25.
H Sakurai H Uchikubo T Yoshimura M Maeda K Kawasaki 《Biochemical and biophysical research communications》1985,130(3):1254-1260
Thiol-containing penta (Leu-Ala-Cys-Ser-Leu) and nona (Leu-Ala-Cys-Ser-Leu-Ile-Glu-Ser-Leu) peptides corresponding to residues 132-136 and 132-140, respectively, of apo-P450 from Psuedomonas putida were synthesized to examine heme-binding by the enzymes in the oxidized (ferric) form. The peptide-hemin complexes prepared in solution were characterized by their optical and ESR spectra. In these complexes without nitrogenous ligands, no ferric complexes in the low-spin state were observed, suggesting that simultaneous axial coordination of Cys-134 (thiolate) and Ser-139 (hydroxyl) of apo-P450cam to the heme is unlikely to occur. In the presence of nitrogenous ligands, such as py, Im and MeIm, the resulting complexes were good models of apo-P450cam-nitrogenous ligand adducts in the low-spin ferric state retaining a thiolate-Fe(III)-nitrogen axial coordination mode, as judged by their spectral pattern as well as by crystal field analysis of ESR g-values. 相似文献
26.
The present study was designed to examine the influence of delta 9-tetrahydrocannabinol (THC) on the central dopaminergic system using circling behavior. THC 5 mg/kg i.p. produced ipsilateral circling in rats with unilateral nigral lesion by 6-hydroxy-dopamine. THC-induced ipsilateral circling was completely antagonized by 0.2 mg/kg of haloperidol. These findings suggest that THC may cause a presynaptic stimulation of nigrostriatal dopaminergic neurons. 相似文献
27.
Effect of Methanol on the Nucleotide Binding to High-Affinity Sites on Chloroplast Coupling Factor 1
The effects of methanol on the nucleotide binding to isolatedchloroplast coupling factor 1 (CF1) were investigated. IsolatedCF1 has four kinds of nucleotide binding sites; a barely dissociableADP-binding site (site A), two slowly exchangeable high-affinitysites with different affinities for ADP (sites B and C) whichare not catalytic sites, and several low-affinity sites (Hisaboriand Sakurai 1984). Methanol at 20% (v/v) slightly acceleratedthe binding of ADP to CF1 but did not influence the number ofbinding sites. Methanol at 1024% (v/v) affected neitherthe total amounts of bound adenine nucleotides (2.5 mol/molCF1) nor the incorporation of labeled ADP from the medium (1.5mol/mol CF1 into the slowly exchangeable sites (sites A, B,C). These results indicate that no appreciable exchange of ADPoccurred at site A at 1024% (v/v) methanol and excludethe possibility of direct participation of nucleotide bindingat this site in the regulation of ATPase. In 32% methanol, theamount of the labeled ADP bound increased, suggesting some exchangeat site A. Methanol at 20% (v/v) greatly increased the affinitiesof sites B and C for ADP, CDP, GDP, UDP and PPi. Conformational change of CF1 induced by the binding of nucleotidesto site(s) B (and C) increased the resistance of CF1 to inactivationby methanol at high concentrations or by cold treatment. (Received August 16, 1984; Accepted January 23, 1985) 相似文献
28.
A 7-year-old girl who was unable to flex her left thumb at the interphalangeal joint proved to have an extremely hypoplastic flexor pollicis longus with normal thenar muscles, which is very rare. The flexor digitorum superficialis of the ring finger was transferred to the inserting portion of the flexor pollicis longus tendon with good results. The patient's cooperation seems to be a factor determining the prognosis. 相似文献
29.
Katsuyuki Haranaka Nobuko Satomi Akiko Sakurai Ruriko Haranaka Naoko Okada Mosaburo Kobayashi 《Cancer immunology, immunotherapy : CII》1985,20(1):1-5
Summary The antitumor activities and capacity for tumor necrosis factor (TNF) production of traditional Chinese herbal preparations (Zhu-ling-tang, Xiao-chai-hu-tang), crude drugs (Polyporus, Hoelen, Bupleuri radix, Angelica radix, Cnidii rhizoma, Cinnamomum cortex), and Krestin (PSK) were investigated. These drugs were given to DDY mice in the drinking water before and after transplantation of Ehrlich tumors, and the development of the intradermally transplanted Ehrlich tumors and survival rate were observed. A good survival rate and sometimes a complete cure were found in the groups administered Bupleuri radix, Xiao-chai-hu-tang, Angelica radix, or Cinnamomum cortex, while the group given Hoelen showed poor results. To examine the capacity for TNF production these drugs were given to DDY mice PO as initial stimulating agents, to stimulate the reticuloendothelial system (RES) prior to lipopolysaccharide injection. The TNF activity was tested from the cytotoxicity against L cells. Significant differences in capacity for TNF production were observed among the drugs. Relatively high levels of TNF activity were noted in the groups given Angelica radix, Bupleuri radix, Cnidii rhizoma, or Cinnamomum cortex, very low activities in the groups given Xiao-chai-hu-tang, Zhu-ling-tang, or Krestin, and no TNF activities in the groups given Polyporus or Hoelen. The TNF capacity for production broadly paralleled the survival rate of the mice transplanted to Ehrlich tumors. Our findings suggest that one mechanism underlying the antitumor activities of these drugs is based on stimulation of the RES and is closely related of TNF production.This work was supported in part by a grant-in-aid from the Ministry of Education, Japan 相似文献
30.
We present a new algorithm for the display of RNA secondarystructure. The principle of the algorithm is entirely differentfrom those currently in use in that our algorithm is objectoriented while currrent algorithms are procedural.The circular RNA molecule of chrysanthemum stunt viroid wasused as input data for demonstrating the operation of the program.The major interest of this method will be found in its potentialuse in simulation graphics of RNA folding processes.
Received on October 9, 1986; accepted on February 17, 1987 相似文献