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171.
Guobin He Debanjan Dhar Hayato Nakagawa Joan Font-Burgada Hisanobu Ogata Yuhong Jiang Shabnam Shalapour Ekihiro Seki Shawn E. Yost Kristen Jepsen Kelly A. Frazer Olivier Harismendy Maria Hatziapostolou Dimitrios Iliopoulos Atsushi Suetsugu Robert M. Hoffman Ryosuke Tateishi Kazuhiko Koike Michael Karin 《Cell》2013
172.
173.
Kaburagi H Sugano N Oshikawa M Koshi R Senda N Kawamoto K Ito K 《Acta biochimica et biophysica Sinica》2007,39(6):399-405
To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1-day-old, 1-week-old, and 2-week-old rats. Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RGU34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expression, and 19 genes with significantly decreased expression. A comprehensive analysis appears to be an effective method of studying the complex process of development. 相似文献
174.
Akinori Masuda Takao Yamanaka Tadatoshi Hirakawa Yasuyuki Koga Ryosuke Minomo Takao Munemoto Chuwa Tei 《BioPsychoSocial medicine》2007,1(1):1-7
Many patients with somatoform disorders are frequently encountered in psychosomatic clinics as well as in primary care clinics.
To assess such patients objectively, the concept of somatosensory amplification may be useful. Somatosensory amplification
refers to the tendency to experience a somatic sensation as intense, noxious, and disturbing. It may have a role in a variety
of medical conditions characterized by somatic symptoms that are disproportionate to demonstrable organ pathology. It may
also explain some of the variability in somatic symptomatology found among different patients with the same serious medical
disorder. It has been assessed with a self-report questionnaire, the Somatosensory Amplification Scale. This instrument was
developed in a clinical setting in the U.S., and the reliability and validity of the Japanese and Turkish versions have been
confirmed as well. 相似文献
175.
Chiba Satoru N. Kakehashi Ryosuke Shibukawa Kouichi Mukai Takahiko Suzuki Yasuyuki Hanzawa Naoto 《Ichthyological Research》2015,62(2):156-162
Ichthyological Research - The geographical distribution and intraspecific genetic diversity of an endangered freshwater goby, Gymnogobius sp. “Chokai-endemic species”, was surveyed... 相似文献
176.
Yoshitaka Tashiro Makoto Urushitani Haruhisa Inoue Masato Koike Yasuo Uchiyama Masaaki Komatsu Keiji Tanaka Maya Yamazaki Manabu Abe Hidemi Misawa Kenji Sakimura Hidefumi Ito Ryosuke Takahashi 《The Journal of biological chemistry》2012,287(51):42984-42994
Evidence suggests that protein misfolding is crucially involved in the pathogenesis of amyotrophic lateral sclerosis (ALS). However, controversy still exists regarding the involvement of proteasomes or autophagy in ALS due to previous conflicting results. Here, we show that impairment of the ubiquitin-proteasome system, but not the autophagy-lysosome system in motor neurons replicates ALS in mice. Conditional knock-out mice of the proteasome subunit Rpt3 in a motor neuron-specific manner (Rpt3-CKO) showed locomotor dysfunction accompanied by progressive motor neuron loss and gliosis. Moreover, diverse ALS-linked proteins, including TAR DNA-binding protein 43 kDa (TDP-43), fused in sarcoma (FUS), ubiquilin 2, and optineurin were mislocalized or accumulated in motor neurons, together with other typical ALS hallmarks such as basophilic inclusion bodies. On the other hand, motor neuron-specific knock-out of Atg7, a crucial component for the induction of autophagy (Atg7-CKO), only resulted in cytosolic accumulation of ubiquitin and p62, and no TDP-43 or FUS pathologies or motor dysfunction was observed. These results strongly suggest that proteasomes, but not autophagy, fundamentally govern the development of ALS in which TDP-43 and FUS proteinopathy may play a crucial role. Enhancement of proteasome activity may be a promising strategy for the treatment of ALS. 相似文献
177.
H Watanabe M Ono H Kimura K Matsumura M Yoshimura Y Okamoto M Ihara R Takahashi H Saji 《Bioorganic & medicinal chemistry letters》2012,22(17):5700-5703
This letter describes the synthesis and biological evaluation of a novel series of radioiodinated oxindole (OI) derivatives for detecting neurofibrillary tangles (NFTs) in the brains of patients with Alzheimer's disease (AD). In binding experiments in vitro, 2-oxindole (2-OI) and 3-oxindole (3-OI) derivatives showed affinity for tau aggregates. The 3-OI derivative 14 showed the highest affinity of these derivatives. In biodistribution experiments using normal mice, the OI derivatives displayed good uptake (2.4-2.5%ID/g at 2min) and clearance from the brain with time (0.6-1.4%ID/g at 30min). In fluorescence staining experiments using AD brain sections, 14 clearly stained NFTs. 3-OI may serve as a new molecular scaffold for developing novel NFT imaging agents. 相似文献
178.
Hakamata W Ishikawa R Ushijima Y Tsukagoshi T Tamura S Hirano T Nishio T 《Bioorganic & medicinal chemistry letters》2012,22(1):62-64
5-Thiazoleacetamide derivatives of AR122 and AR125 were screened as α-glucosidase inhibitors by in silico high-throughput screening from commercial drug-like small compound libraries. Inhibition of α-glucosidase with AR122 and AR125 is time dependent: with no preincubation, AR122 and AR125 are relatively moderate inhibitors, but interestingly, after a 120 min incubation, they were 50-fold more potent (AR122: IC(50)=2.47 μM and AR125: IC(50)=27.1 μM). Plots of ln [residual α-glucosidase activity %] versus preincubation time show a pseudo-first order kinetics for both inhibitors. Through dialysis of enzyme-inhibitor complexes, no activity recovery was shown. These results suggest that AR122 and AR125 constitute a new class of noncarbohydrate mimetic inhibitor with an irreversible mechanism. 相似文献
179.
Kato H Suyama H Yamada R Hasunuma T Kondo A 《Applied microbiology and biotechnology》2012,94(6):1585-1592
To improve the ability of recombinant Saccharomyces cerevisiae strains to utilize the hemicellulose components of lignocellulosic feedstocks, the efficiency of xylose conversion to ethanol needs to be increased. In the present study, xylose-fermenting, haploid, yeast cells of the opposite mating type were hybridized to produce a diploid strain harboring two sets of xylose-assimilating genes encoding xylose reductase, xylitol dehydrogenase, and xylulokinase. The hybrid strain MN8140XX showed a 1.3- and 1.9-fold improvement in ethanol production compared to its parent strains MT8-1X405 and NBRC1440X, respectively. The rate of xylose consumption and ethanol production was also improved by the hybridization. This study revealed that the resulting improvements in fermentation ability arose due to chromosome doubling as well as the increase in the copy number of xylose assimilation genes. Moreover, compared to the parent strain, the MN8140XX strain exhibited higher ethanol production under elevated temperatures (38 °C) and acidic conditions (pH 3.8). Thus, the simple hybridization technique facilitated an increase in the xylose fermentation activity. 相似文献
180.