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311.
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THE high-sulphur proteins of α-keratins, which constitute the non-filamentous matrix between the microfibrils, comprise several major groups of proteins, each group consisting of a number of closely related components. They are obtained in a soluble form by reduction of the disulphide bonds of wool and preferential extraction with alkaline thioglycollate at high ionic strength1. The thiol groups are subsequently stabilized by alkylation with iodoacetic acid. 相似文献
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Serological comparison of bovine T-mycoplasmas 总被引:1,自引:0,他引:1
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Summary The iliofibularis muscle ofXenopus laevis is reported to contain five types of fibres which have different force—velocity relationships. Ten fibres of each type were selected on the basis of succinate dehydrogenase activity, cross-sectional area and location in the muscle, in order to assess the validity of the fibre type classification.Maximum calcium-stimulated myofibrillar ATPase activity (V
max) and apparent Michaelis constant (K
m) for ATP were determined for these 50 fibres from serial sections. The values obtained varied according to the type of fibre. Type 1 had the highest and type 5 the lowest values forK
m andV
max.In a separate experiment, single freeze-dried fibres were used to determine the relationship between their ATP content and apparentK
m for ATP. There was a tendency for high ATP concentrations in fibres with highK
m values.When myofibrillar ATPase activity was related to the maximum velocity of shortening of the five fibre types, a significant correlation was found. It is concluded that calcium-stimulated myofibrillar ATPase histochemistry allows an estimate of the maximum shortening velocity of muscle fibres fromXenopus laevis. 相似文献
320.
David Skelly 《BMC biology》2010,8(1):136
A new study of divergence in freshwater fish provides strong evidence of rapid, temperature-mediated adaptation. This study
is particularly important in the ongoing debate over the extent and significance of evolutionary response to climate change
because divergence has occurred in relatively few generations in spite of ongoing gene flow and in the aftermath of a significant
genetic bottleneck, factors that have previously been considered obstacles to evolution. Climate change may thus be more likely
to foster contemporary evolutionary responses than has been anticipated, and I argue here for the importance of investigating
their possible occurrence. 相似文献