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991.
Azumi K Sabau SV Fujie M Usami T Koyanagi R Kawashima T Fujiwara S Ogasawara M Satake M Nonaka M Wang HG Satou Y Satoh N 《Developmental biology》2007,308(2):572-582
Recent whole-genome studies and in-depth expressed sequence tag (EST) analyses have identified most of the developmentally relevant genes in the urochordate, Ciona intestinalis. In this study, we made use of a large-scale oligo-DNA microarray to further investigate and identify genes with specific or correlated expression profiles, and we report global gene expression profiles for about 66% of all the C. intestinalis genes that are expressed during its life cycle. We succeeded in categorizing the data set into 5 large clusters and 49 sub-clusters based on the expression profile of each gene. This revealed the higher order of gene expression profiles during the developmental and aging stages. Furthermore, a combined analysis of microarray data with the EST database revealed the gene groups that were expressed at a specific stage or in a specific organ of the adult. This study provides insights into the complex structure of ascidian gene expression, identifies co-expressed gene groups and marker genes and makes predictions for the biological roles of many uncharacterized genes. This large-scale oligo-DNA microarray for C. intestinalis should facilitate the understanding of global gene expression and gene networks during the development and aging of a basal chordate. 相似文献
992.
Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension 总被引:10,自引:1,他引:9
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Wong HL Pinontoan R Hayashi K Tabata R Yaeno T Hasegawa K Kojima C Yoshioka H Iba K Kawasaki T Shimamoto K 《The Plant cell》2007,19(12):4022-4034
Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various cellular activities, including plant innate immunity response. In contrast with the large multiprotein NADPH oxidase complex of phagocytes, in plants, only the homologs of the catalytic subunit gp91phox and the cytosolic regulator small GTPase Rac are found. Plant homologs of the gp91phox subunit are known as Rboh (for respiratory burst oxidase homolog). Although numerous Rboh have been isolated in plants, the regulation of enzymatic activity remains unknown. All rboh genes identified to date possess a conserved N-terminal extension that contains two Ca2+ binding EF-hand motifs. Previously, we ascertained that a small GTPase Rac (Os Rac1) enhanced pathogen-associated molecular pattern-induced ROS production and resistance to pathogens in rice (Oryza sativa). In this study, using yeast two-hybrid assay, we found that interaction between Rac GTPases and the N-terminal extension is ubiquitous and that a substantial part of the N-terminal region of Rboh, including the two EF-hand motifs, is required for the interaction. The direct Rac-Rboh interaction was supported by further studies using in vitro pull-down assay, a nuclear magnetic resonance titration experiment, and in vivo fluorescence resonance energy transfer (FRET) microscopy. The FRET analysis also suggests that cytosolic Ca2+ concentration may regulate Rac-Rboh interaction in a dynamic manner. Furthermore, transient coexpression of Os Rac1 and rbohB enhanced ROS production in Nicotiana benthamiana, suggesting that direct Rac-Rboh interaction may activate NADPH oxidase activity in plants. Taken together, the results suggest that cytosolic Ca2+ concentration may modulate NADPH oxidase activity by regulating the interaction between Rac GTPase and Rboh. 相似文献
993.
Kanemitsu Takuya Kawabata Shinji Fukumura Masao Futamura Gen Hiramatsu Ryo Nonoguchi Naosuke Nakagawa Fumiko Takata Takushi Tanaka Hiroki Suzuki Minoru Masunaga Shin-Ichiro Ono Koji Miyatake Shin-Ichi Nakamura Hiroyuki Kuroiwa Toshihiko 《Radiation and environmental biophysics》2019,58(1):59-67
Radiation and Environmental Biophysics - Folic acid (FA) has high affinity for the folate receptor (FR), which is limited expressed in normal human tissues, but over-expressed in several tumor... 相似文献
994.
995.
Yoshihara Toshinori Chang Shuo-wen Tsuzuki Takamasa Natsume Toshiharu Kakigi Ryo Sugiura Takao Naito Hisashi 《Journal of physiology and biochemistry》2019,75(4):585-595
Journal of Physiology and Biochemistry - Males and females of many species, including humans, exhibit different muscle responses and adaptations to exercise stress; however, the molecular... 相似文献
996.
Yusuke Nakatsu Yasuka Matsunaga Takeshi Yamamotoya Koji Ueda Masa-ki Inoue Yu Mizuno Mikako Nakanishi Tomomi Sano Yosuke Yamawaki Akifumi Kushiyama Hideyuki Sakoda Midori Fujishiro Akihide Ryo Hiraku Ono Tohru Minamino Shin-Ichiro Takahashi Haruya Ohno Masayasu Yoneda Tomoichiro Asano 《Cell reports》2019,26(12):3221-3230.e3
997.
Noriko Miyake Hiroyasu Tsukaguchi Eriko Koshimizu Akemi Shono Satoko Matsunaga Masaaki Shiina Yasuhiro Mimura Shintaro Imamura Tomonori Hirose Koji Okudela Kandai Nozu Yuko Akioka Motoshi Hattori Norishige Yoshikawa Akiko Kitamura Hae?Il Cheong Shoji Kagami Michiaki Yamashita Atsushi Fujita Satoko Miyatake Yoshinori Tsurusaki Mitsuko Nakashima Hirotomo Saitsu Kenichi Ohashi Naoko Imamoto Akihide Ryo Kazuhiro Ogata Kazumoto Iijima Naomichi Matsumoto 《American journal of human genetics》2015,96(4):555-564
We investigated complex genomic rearrangements (CGRs) consisting of triplication copy-number variants (CNVs) that were accompanied by extended regions of copy-number-neutral absence of heterozygosity (AOH) in subjects with multiple congenital abnormalities. Molecular analyses provided observational evidence that in humans, post-zygotically generated CGRs can lead to regional uniparental disomy (UPD) due to template switches between homologs versus sister chromatids by using microhomology to prime DNA replication—a prediction of the replicative repair model, MMBIR. Our findings suggest that replication-based mechanisms might underlie the formation of diverse types of genomic alterations (CGRs and AOH) implicated in constitutional disorders. 相似文献
998.
999.