全文获取类型
收费全文 | 3328篇 |
免费 | 191篇 |
出版年
2023年 | 13篇 |
2022年 | 33篇 |
2021年 | 66篇 |
2020年 | 34篇 |
2019年 | 39篇 |
2018年 | 65篇 |
2017年 | 43篇 |
2016年 | 116篇 |
2015年 | 130篇 |
2014年 | 154篇 |
2013年 | 249篇 |
2012年 | 211篇 |
2011年 | 206篇 |
2010年 | 123篇 |
2009年 | 111篇 |
2008年 | 186篇 |
2007年 | 167篇 |
2006年 | 141篇 |
2005年 | 147篇 |
2004年 | 146篇 |
2003年 | 131篇 |
2002年 | 123篇 |
2001年 | 61篇 |
2000年 | 57篇 |
1999年 | 63篇 |
1998年 | 33篇 |
1997年 | 40篇 |
1996年 | 29篇 |
1995年 | 37篇 |
1994年 | 29篇 |
1993年 | 22篇 |
1992年 | 41篇 |
1991年 | 38篇 |
1990年 | 32篇 |
1989年 | 36篇 |
1988年 | 25篇 |
1987年 | 22篇 |
1986年 | 27篇 |
1985年 | 34篇 |
1984年 | 35篇 |
1983年 | 20篇 |
1982年 | 19篇 |
1981年 | 14篇 |
1980年 | 19篇 |
1979年 | 24篇 |
1978年 | 13篇 |
1977年 | 14篇 |
1975年 | 14篇 |
1974年 | 11篇 |
1973年 | 23篇 |
排序方式: 共有3519条查询结果,搜索用时 15 毫秒
31.
32.
33.
34.
35.
Population Ecology - From a field study for the vole population (Clethrionomys rufocanus) in Hokkaido in the late summer of 1965, it has been proved that the range length may decrease from 25 to 18... 相似文献
36.
Mechanism of arachidonic acid liberation in platelet-activating factor-stimulated human polymorphonuclear neutrophils 总被引:1,自引:0,他引:1
S Nakashima A Suganuma M Sato T Tohmatsu Y Nozawa 《Journal of immunology (Baltimore, Md. : 1950)》1989,143(4):1295-1302
Upon stimulation of human polymorphonuclear neutrophils with platelet-activating factor (PAF), arachidonic acid (AA) is released from membrane phospholipids. The mechanism for AA liberation, a key step in the synthesis of biologically active eicosanoids, was investigated. PAF was found to elicit an increase in the cytoplasmic level of free Ca2+ as monitored by fluorescent indicator fura 2. When [3H] AA-labeled neutrophils were exposed to PAF, the enhanced release of AA was observed with a concomitant decrease of radioactivity in phosphatidylinositol and phosphatidylcholine fractions. The inhibitors of phospholipase A2, mepacrine and 2-(p-amylcinnamoyl)-amino-4-chlorobenzoic acid, effectively suppressed the liberation of [3H]AA from phospholipids, indicating that liberation of AA is mainly catalyzed by the action of phospholipase A2. The extracellular Ca2+ is not required for AA release. However, intracellular Ca2+ antagonists, TMB-8 and high dose of quin 2/AM drastically reduced the liberation of AA induced by PAF, indicating that Ca2+ is an essential factor for phospholipase A2 activation. PAF raised the fluorescence of fura 2 at concentrations as low as 8 pM which reached a maximal level about 8 nM, whereas more than nM order concentrations of PAF was required for the detectable release of [3H]AA. Pretreatment of neutrophils with pertussis toxin resulted in complete abolition of AA liberation in response to PAF. However, the fura 2 response to PAF was not effectively inhibited by toxin treatment. In human neutrophil homogenate and membrane preparations, guanosine 5'-O-(thiotriphosphate) stimulated AA release and potentiated the action of PAF. Guanosine 5'-O-(thiodiphosphate) inhibited the effects of guanosine 5'-O-(thiotriphosphate). These results suggest several points: 1) PAF stimulates human polymorphonuclear neutrophils to liberate AA mainly by the action of phospholipase A2; 2) Ca2+ mobilization alone is not sufficient to stimulate AA release, although Ca2+ is the important factor for phospholipase A2 activation; and 3) a pertussis toxin-sensitive GTP-binding protein may be implicated in activation of phospholipase A2. 相似文献
37.
M Watahiki M Tanaka N Masuda K Sugisaki M Yamamoto M Yamakawa J Nagai K Nakashima 《The Journal of biological chemistry》1989,264(10):5535-5539
The perform of chicken prolactin (PRL) deduced from the cDNA sequence contains a signal peptide of 30 amino acid residues followed by a mature PRL of 199 residues. Chicken PRL shows 77, 68, 67, 58, and 31% identity of amino acid sequence with whale, human, ovine, rat, and salmon PRLs, respectively. Elucidation of the primary structure of avian PRL enabled extended analysis of the specific and conserved amino acid residues and domains of the PRL molecules. The mammalian, teleostean, and avian PRLs share 32 common residues, and these conserved residues are observed to cluster in four distinct domains (PD1 to PD4), corresponding to four of five conserved domains of the growth hormones. Of the 32 residues, 8 residues in the PD2 and PD4 domains, including 4 cysteines, are conserved by other members of the growth hormone family, which indicates that these 8 residues may be essential for common structural features of the gene family. On the other hand, 13 other residues distributed among all four domains are conserved almost exclusively in the PRLs, suggesting that these residues are indispensable for specific binding of the PRLs to their receptors. 相似文献
38.
Masahiro Okazaki Nobuyuki Yanagihara Futoshi Izumi Yasuhide Nakashima Akio Kuroiwa 《Journal of neurochemistry》1989,52(1):222-228
The distribution and secretion of atrial natriuretic peptides (ANPs) were investigated in bovine adrenal medulla. (1) Cultured bovine adrenal medullary cells (2 x 10(6)/dish) contained 100.4 +/- 6.0 fmol of immunoreactive ANP (IR-ANP) and 207.3 +/- 6.6 nmol of catecholamines as epinephrine plus norepinephrine. (2) Stimulation of nicotinic but not muscarinic acetylcholine receptors caused a cosecretion of IR-ANP and catecholamines corresponding to the ratio of IR-ANP to catecholamines in cultured bovine adrenal medullary cells. (3) Carbachol-stimulated secretion of IR-ANP was dependent on the presence of extracellular Ca2+. (4) Chromaffin granules isolated from bovine adrenal medulla contained large amounts of IR-ANP and catecholamines, in the same ratio as did cultured adrenal medullary cells. (5) Reverse-phase HPLC analysis showed that both stored and secreted IR-ANP consisted of two components, which eluted at the position of ANP(99-126) or ANP(1-126). These results indicate that ANPs are stored as ANP(99-126) and ANP(1-126) in chromaffin granules, and are cosecreted in parallel with catecholamines in a Ca2+-dependent manner by the stimulation of nicotinic acetylcholine receptors. 相似文献
39.
Double antenna structure of chicken prolactin receptor deduced from the cDNA sequence. 总被引:14,自引:0,他引:14
M Tanaka K Maeda T Okubo K Nakashima 《Biochemical and biophysical research communications》1992,188(2):490-496
Chicken prolactin receptor (cPRLR) deciphered from the cDNA sequence showed a unique double antenna structure in its extracellular domain. The predicted cPRLR preprotein was composed of 831 amino acids and contained a signal peptide and a transmembrane region. The extracellular domain comprised 438 residues, and was divided into two tandemly repeated, highly homologous units, each of which corresponded to the extracellular domains of mammalian prolactin receptors. Both extracellular units of cPRLR possessed two structural features characteristic of the ligand binding units of cytokine/prolactin receptor family, namely two pairs of cysteine residues and a WSXWS motif. These findings strongly suggest that cPRLR contains two repeated ligand binding units, that is a double antenna structure. The cPRLR gene is expressed in a wide range of tissues of laying hen. 相似文献
40.
M Masuda H Nakashima T Ueda H Naba R Ikoma A Otaka Y Terakawa H Tamamura T Ibuka T Murakami 《Biochemical and biophysical research communications》1992,189(2):845-850
Tachyplesin and polyphemusin are antimicrobial peptides recently isolated from the hemocytes of horseshoe crabs (Tachypleus tridentatus and Limulus polyphemus). We synthesized them and their analogs and examined their antiviral activity against human immunodeficiency virus (HIV) type 1 in vitro. The infection of human T cells with the virus was markedly inhibited by some of them at low concentrations. In this structure-activity study, we found that [Tyr5,12, Lys7]-polyphemusin II, which was designated as T22, had extremely high anti-HIV activity. Its 50% inhibitory concentration (EC50) was 0.008 micrograms/ml, while its 50% cytotoxic concentration (CC50) was 54 micrograms/ml and these values were comparable to those of AZT. This result indicates that T22 would be a potential candidate for the therapy of HIV infection. 相似文献