排序方式: 共有107条查询结果,搜索用时 31 毫秒
71.
72.
Moncef Benkhalifa Aicha Madkour Noureddine Louanjli Nouzha Bouamoud Brahim Saadani Ismail Kaarouch 《Expert review of proteomics》2015,12(4):407-423
The development of in vitro fertilization (IVF) techniques for infertility management has led to the investigation of the proteome of follicular fluid and oocyte. In addition, different markers contributing to oocyte maturation and embryo development potential have been reported in the literature. Different techniques were utilized to analyze whole proteome or single protein markers in follicular fluid and oocytes, particularly in animal models. Data from several studies have generated large amounts of information, however, an ideal profile to predict the best oocytes and embryos suitable for implantation are still to be uncovered. The identification of such profiles and markers from follicular fluid, oocytes and endometrium should help scientists and clinicians develop better strategies to improving clinical outcome of IVF cycles. 相似文献
73.
74.
Ben Slama K Ben Sallem R Jouini A Rachid S Moussa L Sáenz Y Estepa V Somalo S Boudabous A Torres C 《Current microbiology》2011,62(6):1794-1801
Fourteen broad-spectrum-cephalosporin-resistant Escherichia
coli isolates were recovered between June and December 2007 in a Tunisian hospital. Genes encoding extended-spectrum-beta-lactamases
(ESBL) and other resistance genes were characterized by PCR and sequencing. The following ESBL genes were identified: bla
CTX-M-15 (12 isolates), bla
CTX-M-14a (one isolate), and bla
CTX-M-14b (one isolate). The bla
OXA-1 gene was detected in 13 bla
CTX-M-producing strains and a bla
TEM-1 gene in 6 of them. The ISEcp1 sequence was found upstream of bla
CTX-M genes in 8 of 14 strains, and orf477 or IS903 downstream of this gene in 13 strains. Nine of the strains carried class 1 integrons and five different gene cassette arrangements
were detected, dfrA17–aadA5 being the most common. One of the strains (bla
CTX-M-14a-positive) harbored three class 1 integrons, and one of them was non-previously described containing as gene cassettes new
variants of aac(6′)-Ib and cmlA1 genes and it was linked to the bla
CTX-M-14a gene flanked by a truncated ISEcp1 sequence (included in GenBank with accession number JF701188). CTX-M-15-producing strains were ascribed to phylogroup B2
(six isolates) and D (six isolates). Multilocus-sequence-typing revealed ten different sequence-types (STs) among ESBL-positive
E.
coli strains with prevalence of ST405 (four strains of phylogroup D) and ST131 types (two strains of phylogroup B2 and serogroup
O25b). A high clonal diversity was also observed among studied strains by pulsed-field-gel-electrophoresis (11 unrelated profiles).
CTX-M-15 is an emergent mechanism of resistance in the studied hospital and the world-disseminated 0:25b-ST131-B2 and ST405-D
clones have been identified among CTX-M-15-producing isolates. 相似文献
75.
Abderrahmani R François A Buard V Tarlet G Blirando K Hneino M Vaurijoux A Benderitter M Sabourin JC Milliat F 《PloS one》2012,7(4):e35740
Normal tissue toxicity still remains a dose-limiting factor in clinical radiation therapy. Recently, plasminogen activator inhibitor type 1 (SERPINE1/PAI-1) was reported as an essential mediator of late radiation-induced intestinal injury. However, it is not clear whether PAI-1 plays a role in acute radiation-induced intestinal damage and we hypothesized that PAI-1 may play a role in the endothelium radiosensitivity. In vivo, in a model of radiation enteropathy in PAI-1 -/- mice, apoptosis of radiosensitive compartments, epithelial and microvascular endothelium was quantified. In vitro, the role of PAI-1 in the radiation-induced endothelial cells (ECs) death was investigated. The level of apoptotic ECs is lower in PAI-1 -/- compared with Wt mice after irradiation. This is associated with a conserved microvascular density and consequently with a better mucosal integrity in PAI-1 -/- mice. In vitro, irradiation rapidly stimulates PAI-1 expression in ECs and radiation sensitivity is increased in ECs that stably overexpress PAI-1, whereas PAI-1 knockdown increases EC survival after irradiation. Moreover, ECs prepared from PAI-1 -/- mice are more resistant to radiation-induced cell death than Wt ECs and this is associated with activation of the Akt pathway. This study demonstrates that PAI-1 plays a key role in radiation-induced EC death in the intestine and suggests that this contributes strongly to the progression of radiation-induced intestinal injury. 相似文献
76.
Imen Tarchoune Elena Degl’Innocenti Rym Kaddour Lucia Guidi Mokhtar Lachaâl Flavia Navari-Izzo Zeineb Ouerghi 《Acta Physiologiae Plantarum》2012,34(2):607-615
Basil (Ocimum basilicum L., cultivar Genovese) plants were grown in Hoagland solution with or without 50 mM NaCl or 25 mM Na2SO4. After 15 days of treatment, Na2SO4 slowed growth of plants as indicated by root, stem and leaf dry weight, root length, shoot height and leaf area, and the
effects were major of those induced by NaCl. Photosynthetic response was decreased more by chloride salinity than by sulphate.
No effects in both treatments on leaf chlorophyll content, maximal efficiency of PSII photochemistry (F
v/F
m) and electron transport rate (ETR) were recorded. Therefore, an excess of energy following the limitation to CO2 photoassimilation and a down regulation of PSII photochemistry was monitored under NaCl, which displays mechanisms that play
a role in avoiding PSII photodamage able to dissipate this excess energy. Ionic composition (Na+, K+, Ca2+, and Mg2+) was affected to the same extent under both types of salinity, thus together with an increase in leaves Cl−, and roots SO4
2− in NaCl and Na2SO4-treated plants, respectively, may have resulted in the observed growth retardation (for Na2SO4 treatment) and photosynthesis activity inhibition (for NaCl treatment), suggesting that those effects seem to have been due
to the anionic component of the salts. 相似文献
77.
78.
Gnouma Asma Sehli Emna Medhioub Walid Ben Dhieb Rym Masri Mahmoud Mehlmer Norbert Slimani Wissem Sebai Khaled Zouari Amel Brück Thomas Medhioub Amel 《Bioprocess and biosystems engineering》2018,41(10):1449-1459
Bioprocess and Biosystems Engineering - Microalgae could be of importance for future biodiesel production as an alternative for a third generation of biofuels. To select the most appropriate strain... 相似文献
79.
80.
Aouameur R Da Cal S Bissonnette P Coady MJ Lapointe JY 《American journal of physiology. Gastrointestinal and liver physiology》2007,293(6):G1300-G1307
This study presents the characterization of myo-inositol (MI) uptake in rat intestine as evaluated by use of purified membrane preparations. Three secondary active MI cotransporters have been identified; two are Na(+) coupled (SMIT1 and SMIT2) and one is H(+) coupled (HMIT). Through inhibition studies using selective substrates such as d-chiro-inositol (DCI, specific for SMIT2) and l-fucose (specific for SMIT1), we show that SMIT2 is exclusively responsible for apical MI transport in rat intestine; rabbit intestine appears to lack apical transport of MI. Other sugar transport systems known to be present in apical membranes, such as SGLT1 or GLUT5, lacked any significant contribution to MI uptake. Functional analysis of rat SMIT2 activity, via electrophysiological studies in Xenopus oocytes, demonstrated similarities to the activities of SMIT2 from other species (rabbit and human) displaying high affinities for MI (0.150 +/- 0.040 mM), DCI (0.31 +/- 0.06 mM), and phlorizin (Pz; 0.016 +/- 0.007 mM); low affinity for glucose (36 +/- 7 mM); and no affinity for l-fucose. Although these functional characteristics essentially confirmed those found in rat intestinal apical membranes, a unique discrepancy was seen between the two systems studied in that the affinity constant for glucose was approximately 40-fold lower in vesicles (K(i) = 0.94 +/- 0.35 mM) than in oocytes. Finally, the transport system responsible for the basolateral efflux transporter of glucose in intestine, GLUT2, did not mediate any significant radiolabeled MI uptake in oocytes, indicating that this transport system does not participate in the basolateral exit of MI from small intestine. 相似文献