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241.
Linda Spiegelberg Sigrid MA Swagemakers Wilfred FJ van IJcken Edwin Oole Eppo B Wolvius Jeroen Essers Joanna AM Braks 《Molecular medicine (Cambridge, Mass.)》2014,20(1):257-269
A side effect of radiation therapy in the head and neck region is injury to surrounding healthy tissues such as irreversible impaired function of the salivary glands. Hyperbaric oxygen therapy (HBOT) is clinically used to treat radiation-induced damage but its mechanism of action is largely unknown. In this study, we investigated the molecular pathways that are affected by HBOT in mouse salivary glands two weeks after radiation therapy by microarray analysis. Interestingly, HBOT led to significant attenuation of the radiation-induced expression of a set of genes and upstream regulators that are involved in processes such as fibrosis and tissue regeneration. Our data suggest that the TGFβ-pathway, which is involved in radiation-induced fibrosis and chronic loss of function after radiation therapy, is affected by HBOT. On the longer term, HBOT reduced the expression of the fibrosis-associated factor α-smooth muscle actin in irradiated salivary glands. This study highlights the potential of HBOT to inhibit the TGFβ-pathway in irradiated salivary glands and to restrain consequential radiation induced tissue injury. 相似文献
242.
Paolo Maltese Emanuele Canestrari Linda Palma Annamaria Ruzzo Francesco Corini Michele Menotta Francesca Andreoni Anna Latiano Vito Annese Mauro Magnani 《The Journal of steroid biochemistry and molecular biology》2009,113(3-5):269-274
Polymorphisms in the glucocorticoid receptor (GR) gene have been associated with altered sensitivity to glucocorticoids. We designed a high-resolution melting (HRM) assay to detect, simultaneously, the three most intriguing GR polymorphisms, selected on the bases of clinical relevance and frequencies in caucasian population as described in literature. HRM enables the detection of ER22/23EK and N363S genotypes but fails to discriminate homozygous mutant for the BclI polymorphism from wild-type samples, however a simple spike experiment leads to a clear discrimination between these genotypes. The analyses were performed on a cohort of 70 healthy Caucasian subjects. The method was validated by restriction fragment length polymorphisms; HRM results were found to be in 100% concordance with those observed with the restriction enzymes. We also employed this method on a population of 40 Crohn Disease patients; the analysis demonstrated a significantly higher frequency of the BclI polymorphism in patients than in healthy volunteers.This is, at now, the less expensive and time-and work-saving method to detect GR mutations, providing precision, fast screening and high throughput capabilities. 相似文献
243.
Rogier AM Quax Ya?l A de Man Jan W Koper Elisabeth FC van Rossum Sten P Willemsen Steven WJ Lamberts Johanna MW Hazes Radboud JEM Dolhain Richard A Feelders 《Arthritis research & therapy》2012,14(4):R183
Introduction
The mechanism underlying the spontaneous improvement of rheumatoid arthritis (RA) during pregnancy and the subsequent postpartum flare is incompletely understood, and the disease course varies widely between pregnant RA patients. In pregnancy, total and free levels of cortisol increase gradually, followed by a postpartum decrease to prepregnancy values. The glucocorticoid receptor (GR) polymorphisms BclI and N363S are associated with relatively increased glucocorticoid (GC) sensitivity, whereas the 9β and ER22/23EK polymorphisms of the GR gene are associated with a relatively decreased GC sensitivity. We examined the relation between the presence of these GR polymorphisms and level of disease activity and disease course of RA during pregnancy and postpartum.Methods
We studied 147 participants of the PARA study (Pregnancy-Induced Amelioration of Rheumatoid Arthritis study), a prospective study investigating the natural improvement during pregnancy and the postpartum flare in women with RA. Patients were visited, preferably before pregnancy, at each trimester and at three postpartum time points. On all occasions, disease activity was scored by using DAS28. All patients were genotyped for the GR polymorphisms BclI, N363S, 9β, and ER22/23EK and divided in groups harboring either polymorphisms conferring increased GC sensitivity (BclI and N363S; GC-S patients) or polymorphisms conferring decreased GC sensitivity (9β or 9β + ER22/23EK; GC-I patients). Data were analyzed by using a mixed linear model, comparing GC-S patients with GC-I patients with respect to improvement during pregnancy and the postpartum flare. The cumulative disease activity was calculated by using time-integrated values (area under the curve, AUC) of DAS28 in GC-I patients versus GC-S patients. Separate analyses were performed according to the state of GC use.Results
GC-S patients treated with GC had a significantly lower AUC of DAS28 in the postpartum period than did GC-I patients. This difference was not observed in patients who were not treated with GCs. During pregnancy, GC-S and GC-I patients had comparable levels of disease activity and course of disease.Conclusions
Differences in relative GC sensitivity, as determined by GR polymorphisms, are associated with the level of disease activity in the postpartum period in GC-treated patients, but they do not seem to influence the course of the disease per se. 相似文献244.
245.
K van Oers A W Santure I De Cauwer N EM van Bers R PMA Crooijmans B C Sheldon M E Visser J Slate M AM Groenen 《Heredity》2014,112(3):307-316
Linking variation in quantitative traits to variation in the genome is an important, but
challenging task in the study of life-history evolution. Linkage maps provide a valuable
tool for the unravelling of such trait−gene associations. Moreover, they give
insight into recombination landscapes and between-species karyotype evolution. Here we
used genotype data, generated from a 10k single-nucleotide polymorphism (SNP) chip, of
over 2000 individuals to produce high-density linkage maps of the great tit (Parus
major), a passerine bird that serves as a model species for ecological and
evolutionary questions. We created independent maps from two distinct populations: a
captive F2-cross from The Netherlands (NL) and a wild population from the United Kingdom
(UK). The two maps contained 6554 SNPs in 32 linkage groups, spanning 2010 cM and
1917 cM for the NL and UK populations, respectively, and were similar in size and
marker order. Subtle levels of heterochiasmy within and between chromosomes were
remarkably consistent between the populations, suggesting that the local departures from
sex-equal recombination rates have evolved. This key and surprising result would have been
impossible to detect if only one population was mapped. A comparison with zebra finch
Taeniopygia guttata, chicken Gallus gallus and the green anole lizard
Anolis carolinensis genomes provided further insight into the evolution of
avian karyotypes. 相似文献
246.
M Gennarelli G Novelli A Ruzzo F Grianti B Dallapiccola 《Bollettino della Società italiana di biologia sperimentale》1989,65(1):13-18
An inexpensive apparatus for Pulsed Field Gel Electrophoresis constructed by the Authors, is described. According to our experimental condition it was found that this apparatus is capable to resolve DNA fragments in order of 50-1500 Kb. This range of analysis is adequate for the molecular studies adjacent regions of prokaryotes, primitive eukaryotes and mammals. 相似文献
247.
248.
249.
Background
Pelodera (Rhabditis) strongyloides is a small saprophytic nematode that lives in decaying organic matter. On rare occasions, it can invade the mammalian skin, causing a pruritic, erythematous, alopecic and crusting dermatitis on skin sites that come into contact with the ground. Diagnosis of the disease is based on case history (a dog living outdoors on damp straw bedding) with characteristic skin lesions and on the demonstration of typical larvae in skin scrapings or biopsy. Pelodera (rhabditic) dermatitis cases have been reported mainly from Central European countries and the United States. 相似文献250.
Bases and spaces: resources on the web for accessing the draft human genome - II - After publication of the draft 下载免费PDF全文
Colin AM Semple 《Genome biology》2001,2(6):reviews2001.1-reviews20016
The volume of human genome sequence and the variety of web-based tools to access it continue to grow at an impressive rate, but a working knowledge of certain key resources can be sufficient to get the most from your genome. This article provides an update to Genome Biology 2000, 1(4):reviews2001.1-2001.5. 相似文献