Tandem repeats modify the structure of the canine CD1D gene

Among the CD1 proteins that present lipid antigens to T cells, CD1d is the only one that stimulates a population of T cells with an invariant T‐cell receptor known as NKT cells. Sequencing of a 722 nucleotide gap in the dog (Canis lupus familiaris) genome revealed that the canine CD1D gene lacks a sequence homologous to exon 2 of human CD1D, coding for the start codon and signal peptide. Also, the canine CD1D gene contains three different short tandem repeats that disrupt the expected gene structure. Because canine CD1D cDNA lacks sequences homologous to human exon 2 and 3, the functionality of canine CD1d protein may be affected, and this could have consequences for the development and activation of canine NKT cells.     

Ultrastructural localization of a chondroitinase-sensitive, cuprolinic blue-positive filament in developing mouse lung

By use of the cationic dye Cuprolinic Blue in a critical electrolyte concentration method, the lungs of mice ranging from the late fetal stage (17 days of gestation) to the puberal stage (27 days) were surveyed for their proteoglycans. A large Cuprolinic Blue-positive filament is present within the connective tissue of lungs of late fetal and young postnatal mice. It is mostly located at the boundary between large extracellular matrix structures and electron microscopically empty areas, but sometimes also at the surface of fibroblast-like cells. The stainability of the filament disappears after treatment with chondroitinase ABC or chondroitinase AC, but not after treatment with nitrous acid. The Cuprolinic Blue-positive structure appears to be most abundant around 2 days postnatally. From day 10 on, its number decreases dramatically, and it can be no longer observed in the lungs of 27-day-old mice.     

27 Spatiotemporal distribution of abundance and density of calcareous green macroalgae along the florida keys: a synchrony study

The development of sustainable integrated aquaculture systems requires combining fed aquaculture (finfish) with extractive inorganic aquaculture (seaweed) and extractive organic aquaculture (shellfish). With the support of AquaNet, the Network of Centers of Excellence in Aquaculture in Canada, we are developing such a system at an industrial pilot scale by co-cultivating salmon (Salmo salar), kelp ( Laminaria saccharina ) and blue mussel ( Mytilus edulis ) at aquaculture sites in the Bay of Fundy, Canada. This presentation will focus on the development of the extractive inorganic component. The entire cycle of rearing Laminaria saccharina has been completed and improved, both in the laboratory and at the integrated sites: release in the laboratory of spores from mature macroscopic sporophytes, seeding of ropes, germination of microscopic gametophytes, sexual maturation of male and female gametophytes, development of zygotes into juvenile sporophytes, which are then transplanted to the sites for rapid grow-out. Another aspect of the project, food safety monitoring of chemical therapeutants and phycotoxins in mussel and kelp cultured in proximity to salmon, will also be described. The productivity, nutrient absorption capacity, and role of the seaweed component are being analyzed so that its appropriate scale to the other components can be defined in order to develop responsible aquaculture practices in which metabolic/physiological processes of the different co-cultured organisms counter-balance each other within acceptable operational limits. Adopting polytrophic strategies will be key to the aquaculture industry to develop its environmentally and economically-balanced diversification and increase its social acceptability within a broader coastal management framework.     

A general method for selection of riboflavin-overproducing food grade micro-organisms

Background  

This study describes a strategy to select and isolate spontaneous riboflavin-overproducing strains of Lactobacillus (Lb.) plantarum, Leuconostoc (Lc.) mesenteroides and Propionibacterium (P.) freudenreichii.     

Kinetics of uranous iron and ferrous iron oxidation by thiobacillus ferrooxidans

Archives of Microbiology -