全文获取类型
收费全文 | 6058篇 |
免费 | 567篇 |
国内免费 | 2篇 |
专业分类
6627篇 |
出版年
2022年 | 54篇 |
2021年 | 117篇 |
2020年 | 63篇 |
2019年 | 67篇 |
2018年 | 78篇 |
2017年 | 60篇 |
2016年 | 134篇 |
2015年 | 266篇 |
2014年 | 262篇 |
2013年 | 390篇 |
2012年 | 485篇 |
2011年 | 442篇 |
2010年 | 258篇 |
2009年 | 210篇 |
2008年 | 373篇 |
2007年 | 384篇 |
2006年 | 323篇 |
2005年 | 275篇 |
2004年 | 281篇 |
2003年 | 277篇 |
2002年 | 276篇 |
2001年 | 72篇 |
2000年 | 43篇 |
1999年 | 62篇 |
1998年 | 86篇 |
1997年 | 53篇 |
1996年 | 50篇 |
1995年 | 41篇 |
1994年 | 54篇 |
1993年 | 44篇 |
1992年 | 37篇 |
1991年 | 32篇 |
1990年 | 45篇 |
1989年 | 35篇 |
1988年 | 34篇 |
1986年 | 29篇 |
1985年 | 39篇 |
1984年 | 37篇 |
1983年 | 40篇 |
1982年 | 41篇 |
1981年 | 42篇 |
1980年 | 33篇 |
1978年 | 27篇 |
1977年 | 25篇 |
1976年 | 33篇 |
1975年 | 29篇 |
1974年 | 28篇 |
1973年 | 27篇 |
1972年 | 24篇 |
1969年 | 25篇 |
排序方式: 共有6627条查询结果,搜索用时 15 毫秒
61.
D. M. McLean E. Joan Quantz Ruth D. Bach B. Mae Pevzner R. P. B. Larke G. A. McNaughton 《CMAJ》1964,90(25):1390-1393
Virological investigations of 115 children with the aseptic meningitis syndrome during 1963 resulted in the isolation of enteroviruses from cerebrospinal fluid (CSF) and/or feces of 21 of 48 children who had no association with mumps. For the third successive year, Echo 9 was the dominant enterovirus in cases of aseptic meningitis in Toronto children, but no rashes were associated with Echo 9 meningitis during 1963, in contradistinction to previous years. Mumps virus was isolated from CSF of 25 patients by inoculation of rhesus monkey kidney cultures, and rising or elevated mumps antihemagglutinin titres in paired sera from a further 33 cases provided laboratory evidence of infection with mumps virus in 58 of 67 patients with mumps meningoencephalitis. No enlargement of salivary glands was noted in 20 laboratory-proved cases of mumps meningoencephalitis. Enteroviral meningitis occurred principally during summer, but the peak of mumps meningoencephalitis occurred during late winter. 相似文献
62.
We have examined the nature of the circular dichroism band at 330 mμ for a series of copolymers of β-p-nitrobenzyl L -aspartate with β-benzyl L -aspartate. The circular dichroism band arises from an electronic transition in the nitroaromatic group. In order to interpret the effect quantitatively, we employed a simplified statistical treatment and curve fitting for six copolymers. Both approaches gave consistent results, which indicates that the dichroism comes from pairwise interactions between two nitrobenzyl groups. We constructed a molecular model that meets the constraints and requirements of the analyses developed in this paper. In this model, it is proposed that the main chain forms a right-handed α-helix and that nitrobenzyl groups separated by four residues interact with each other. 相似文献
63.
Zusammenfassung Es werden verschiedene Typen von Einschlußkörperchen im Karyoplasma der Epithelzellen des menschlichen Nebenhodenepithels beschrieben. Die verschieden strukturierten Kerninklusionen werden als eine Differenzierungsreihe aufgefaßt. Sie beginnt mit kleinen rundlichen Körperchen aus feinfädigem Material und führt über die Ausbildung von Anhäufungen dichter homogener Kugeln zu großen Vakuolen wechselnden Inhaltes.
Herrn Professor Dr. W. Bargmann zum 60. Geburtstag gewidmet.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
Summary Different types of inclusion bodies are described in the karyoplasm of the epithelial cells of the human epididymis. The structural differences between these inclusions are interpreted as being indicative of consecutive stages in the process of their formation. Thus small, spherical bodies consisting of a fine fibrous material are believed to be the initial stages in the formation of the inclusion bodies whereas the dense, homogenous globules are thought to represent a later stage. Large vacuoles containing different materials are regarded as the final stage in this process of differentiation.
Herrn Professor Dr. W. Bargmann zum 60. Geburtstag gewidmet.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
64.
The Electrophoretic Velocity of Human Red Cells, of Their Ghosts and Mechanically Produced Fragments, and of Certain Lipid Complexes 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The Journal of general physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ghosts prepared in CO2-saturated water from unwashed human red cells can be fragmented mechanically, but ghosts from thrice washed cells cannot. If the ghosts are prepared by freezing and thawing, this difference is not observed. The electrophoretic velocity varies also with the way in which the ghosts are prepared. The pH-mobility dependence of washed red cells flatten off to a plateau at pH 9, and the electrophoretic velocity is zero at about pH 2. Ghosts prepared by freezing and thawing have almost the same pH-mobility dependence, but if the ghosts are prepared in CO2-saturated hyptonic saline, the mobility at pH 9.4 is 0.75 times that of washed cells. Fragments of ghosts of unwashed red cells have a smaller mobility than that of the red cells. Trypsin reduces the mobility of washed red cells and of ghosts. Sols of lipid complexes (lecithin, cephalin, and lipositol), at varying pH's, have a mobility 1.2 times that of the washed red cell. The pH-mobility relation is otherwise similar. These complexes can be coated with dextran and trypsin. 相似文献
65.
Rapid Differentiation Between Nocardia and Streptomyces by Paper Chromatography of Whole-Cell Hydrolysates 总被引:24,自引:11,他引:13
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
B. Becker Mary P. Lechevalier Ruth E. Gordon H. A. Lechevalier 《Applied microbiology》1964,12(5):421-423
Whole-cell hydrolysates were prepared from 58 strains of nocardiae and streptomycetes. Strains morphologically intermediate between the two genera and morphological variants of the same strains were included. Paper chromatograms made from the whole-cell hydrolysates clearly demonstrated meso-diaminopimelic acid as a major constituent of cultures of Nocardia spp., and LL-diaminopimelic acid as a major constituent of cultures of Streptomyces spp. In cultures of ten strains of N. madurae and of three of N. pelletieri, meso-diaminopimelic acid predominated, thereby supporting the assignment of these species to the genus Nocardia. 相似文献
66.
67.
Summary Three non-chromosomal and two chromosomal genes which influence resistance to streptomycin are described. Each of the non-chromosomal factors,sr-500,sr-1500, andsd, exhibits uniparental inheritance, with all progeny receiving the factor when it is carried by the parent of mating-typeplus, and none when it is carried by the mating-typeminus parent. The streptomycin-dependence factor,sd, shows zygotic dominance when contributed by the mating-typeplus parent, but not when coming from the mating-typeminus parent, indicating that the uniparental transmission results from events occurring within the zygote early in maturation and well before meiosis. The chromosomal geneA interacts both with chromosomal and non-chromosomal genes at the biochemical level, but does not alter their patterns of inheritance.With 1 Figure in the TextThis paper is dedicated to ProfessorL. C. Dunn in gratitude to him as teacher and advisor, on the occasion of his retirement.This work was supported by grants from the U.S. Public Health Service and the National Science Foundation. The generosity and interest of ProfessorFrancis J. Ryan in providing laboratory space is gratefully acknowledged, as is the technical assistance of MissFran Yablonsky. 相似文献
68.
Ruth Schwaninger Eric Dumermuth M. Ernst Schweingruber 《Molecular & general genetics : MGG》1990,221(3):403-410
Summary Structural gene mutants of the cell-surface glycoprotein acid phosphatase of Schizosaccharomyces pombe were analysed to define structural determinants that are responsible for enzymatic activity, N-glycosylation and secretion. All seven defined mutations cause a single amino acid substitution in the mature acid phosphatase protein and destroy the enzymatic activity. The mutational lesions are distributed throughout the pho1 gene. A ser to phe substitution at position 349 abolishes enzymatic activity only and does not affect glycosylation and secretion. Two mutations create a new N-glycosylation site by substitution of pro at position 56 by phe and ser, respectively. This new site is apparently used in the mutants. Their core-glycosylated acid phosphatase is slightly larger than that of the wild type. Overglycosylation seems not to affect secretion. Four different mutations (a gly to asp substitution at position 281 and ser to phe substitutions at positions 150, 271 and 277) cause intracellular accumulation of enzymatically inactive core-glycosylated acid phosphatase precursor. These mutational lesions apparently block transport of acid phosphatase from the endoplasmic reticulum to the Golgi apparatus. 相似文献
69.
70.