全文获取类型
收费全文 | 9542篇 |
免费 | 925篇 |
国内免费 | 3篇 |
专业分类
10470篇 |
出版年
2022年 | 80篇 |
2021年 | 169篇 |
2020年 | 103篇 |
2019年 | 105篇 |
2018年 | 114篇 |
2017年 | 111篇 |
2016年 | 210篇 |
2015年 | 378篇 |
2014年 | 404篇 |
2013年 | 546篇 |
2012年 | 706篇 |
2011年 | 633篇 |
2010年 | 385篇 |
2009年 | 324篇 |
2008年 | 528篇 |
2007年 | 594篇 |
2006年 | 487篇 |
2005年 | 407篇 |
2004年 | 418篇 |
2003年 | 417篇 |
2002年 | 389篇 |
2001年 | 134篇 |
2000年 | 100篇 |
1999年 | 104篇 |
1998年 | 135篇 |
1997年 | 94篇 |
1996年 | 92篇 |
1995年 | 78篇 |
1994年 | 93篇 |
1993年 | 78篇 |
1992年 | 92篇 |
1991年 | 63篇 |
1990年 | 92篇 |
1989年 | 80篇 |
1988年 | 90篇 |
1987年 | 60篇 |
1986年 | 62篇 |
1985年 | 79篇 |
1984年 | 86篇 |
1983年 | 71篇 |
1982年 | 62篇 |
1981年 | 81篇 |
1980年 | 58篇 |
1979年 | 54篇 |
1978年 | 58篇 |
1977年 | 48篇 |
1976年 | 58篇 |
1975年 | 47篇 |
1974年 | 65篇 |
1973年 | 43篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
21.
22.
23.
24.
In vitro quantitative autoradiography of high-affinity [3H]imipramine binding sites was performed on 16 human brains postmortem. The densities of binding sites were highest in the hypothalamus. Next, in descending order, were the basal and lateral nuclei of the amygdala; substantia innominata; insular cortex; the central nucleus of the amygdala; the anterior nucleus of the thalamus; the head of the caudate nucleus; portions of the frontal, parietal, and temporal cortex; claustrum; the granular layer of the dentate gyrus; substantia nigra; the pyramidal layer of CA fields; globus pallidus; red nucleus; and white matter. Imipramine binding was found to increase with age in a region-specific manner. The presence of alcohol had a similar effect, which was most pronounced in the hippocampus. Sex and time from death to autopsy did not affect imipramine binding, in our sample. 相似文献
25.
Drosophila basement membrane procollagen IV. I. Protein characterization and distribution 总被引:1,自引:0,他引:1
G P Lunstrum H P B?chinger L I Fessler K G Duncan R E Nelson J H Fessler 《The Journal of biological chemistry》1988,263(34):18318-18327
A collagen was isolated from Drosophila E85, Schneider line 2L and Kc cell cultures. The purified protein was characterized and antibodies were raised against it. Immunofluorescence microscopy locates this material to the regions of basement membranes of Drosophila embryos, larvae, and adults. The molecules are mostly, or entirely, homotrimers of one polypeptide chain linked by interchain disulfide bonds. The partial amino acid sequences of a cyanogen bromide cleavage product of this chain are identical with a part of the virtual translation product of the Drosophila pro alpha 1(IV) nucleotide sequence that is reported in the accompanying paper. This gene is at Drosophila chromosome location 25C and was identified by the high homology of one part of it with the noncollagenous carboxyl terminus (NC1) of vertebrate type IV basement membrane collagens (Blumberg, B., MacKrell, A. J., Olson, P. F., Kurkinen, M., Monson, J. M., Natzle, J. E., and Fessler, J. H. (1987) J. Biol. Chem. 262, 5947-5950). In the electron microscope each molecule appears as a thread with a knob at one end, which contains the carboxyl peptide domains. The variation of flexibility of the thread was mapped along its length. Pulse-chase labeling of cell cultures showed that these molecules associate into disulfide-linked dimers and higher oligomers that can be partly separated by velocity sedimentation and are resolved by sodium dodecyl sulfate-agarose gel electrophoresis. Dimers and higher oligomers formed by overlap of the amino ends of molecules were found. Mild pepsin digestion of Drosophila embryos and larvae solubilized the corresponding disulfide-linked collagen molecules, and Staphylococcus aureus V8 protease peptide maps showed the identity of the collagen derived from animals and from cell cultures. Individual, native molecules have a sedimentation coefficient s20,w = 4.1 S, the dichroic spectrum and amino acid composition of a collagen, and a Tm = 31 degrees C. Positive in situ hybridization with a specific probe for this collagen began 6-8 h after egg laying and showed message in the locations of embryos and larvae which reacted with the antibodies. This included some prominent individual cells in the hemolymph. 相似文献
26.
C J Duncan C L Osborne J J Parsons K E McCall M J Jackson 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1988,90(2):459-460
1. Isolated amphibian hearts and pectoris cutaneous muscles were exposed either to DNP or to caffeine, thereby producing severe myofilament damage. 2. No accompanying change in sarcolemma permeability was detected by monitoring either CK or LDH release or Procion yellow entry in the heart, or by Procion entry in amphibian skeletal muscle. 3. The findings are in contrast with mammalian cardiac and skeletal muscles, and confirm that the pathways leading to myofilament degradation and to the breakdown in sarcolemma organization are separate. 相似文献
27.
An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated on an automated DNA synthesizer, contains high levels of attached DNA, and has superior mechanical properties. It should be generally useful for affinity chromatography of DNA binding proteins. 相似文献
28.
29.
Development Genes and Evolution - 相似文献
30.
Ruth C. Steinkamp Clarence L. Young Dolores Nyhus Arnold E. Greenberg 《The Western journal of medicine》1968,109(2):126-133
The amount of sodium ion in water used for ingestion may be critical in effective use of a low sodium dietary regimen. Waters containing not over 20 mg of sodium per liter are provided for in the sodium restricted diets set forth by the American Heart Association. For diets containing more than 500 mg of sodium a day, waters of greater sodium content may be used if proper dietary adjustments are made.While assessment of the long-term average sodium content of a community water supply is difficult, the determined values for sodium lend to classification within range categories. The larger community water supplies in California are presented within several range categories of sodium content.The more commonly used water softeners add sodium to water. The sodium-restricted patient should be cautioned against their use. Similar consideration should probably be given to water supplies of retirement communities where the potential for disorders requiring sodium restriction is greater than in the general population. 相似文献