A new aglaspidid arthropod from the Lower Ordovician of Wales

A new genus and species of aglaspidid arthropod, Tremaglaspis unite , is described from the well-known upper Tremadoc locality of Y Garth in North Wales, and compared with other Cambrian and Ordovician taxa. It is remarkable in having a large hypostome, a character shared with other primitive arachnomorphs.     

Phylogenetic diversity of stress signalling pathways in fungi

Background  

Microbes must sense environmental stresses, transduce these signals and mount protective responses to survive in hostile environments. In this study we have tested the hypothesis that fungal stress signalling pathways have evolved rapidly in a niche-specific fashion that is independent of phylogeny. To test this hypothesis we have compared the conservation of stress signalling molecules in diverse fungal species with their stress resistance. These fungi, which include ascomycetes, basidiomycetes and microsporidia, occupy highly divergent niches from saline environments to plant or mammalian hosts.     

An updated 18S rRNA phylogeny of tunicates based on mixture and secondary structure models

Background  

Tunicates have been recently revealed to be the closest living relatives of vertebrates. Yet, with more than 2500 described species, details of their evolutionary history are still obscure. From a molecular point of view, tunicate phylogenetic relationships have been mostly studied based on analyses of 18S rRNA sequences, which indicate several major clades at odds with the traditional class-level arrangements. Nonetheless, substantial uncertainty remains about the phylogenetic relationships and taxonomic status of key groups such as the Aplousobranchia, Appendicularia, and Thaliacea.     

Vegetation change in an ombrotrophic mire in northern England after excluding sheep

Abstract. The role of sheep grazing on vegetation change in upland mires removed from livestock farming and surrounded by conifer plantation was investigated with a grazing trial at Butterburn Flow in northern England. Paired grazed and ungrazed plots from central and peripheral locations were compared over 14 yr. Vegetation data from 34 mires in Kielder Forest provided an ordination framework within which vegetation trends were investigated. A gradient from dry moorland/hummock to wet mire/hollow vegetation dominated this framework and may reflect hydrological variability and structural vegetation differences between the mires. Some species were significantly affected by change in grazing intensity and there were differences between the edge and the centre of the mire. Overall vegetation change depended upon the grazing management and the position of the plots such that the removal of sheep grazing decreased the cover of species typical of wet ombrotrophic conditions, but only at the periphery of the mire. The vegetation in one plot became very similar to that of mires elsewhere in Kielder Forest where sheep were removed several decades ago. Cessation of grazing on upland mires is likely to lead to slow structural and species change in vegetation at the mire edge with a long‐term loss of ombrotrophic species. The nature conservation significance of these changes will depend upon whether or not management objectives target natural conditions or wish to maximize ombrotrophic vegetation. The context of external factors such as climate and pollution may, however, be more important in determining site condition on the wettest mires.     

Influence of Tertiary paleoenvironmental changes on the diversification of South American mammals: a relaxed molecular clock study within xenarthrans

Background  

Comparative genomic data among organisms allow the reconstruction of their phylogenies and evolutionary time scales. Molecular timings have been recently used to suggest that environmental global change have shaped the evolutionary history of diverse terrestrial organisms. Living xenarthrans (armadillos, anteaters and sloths) constitute an ideal model for studying the influence of past environmental changes on species diversification. Indeed, extant xenarthran species are relicts from an evolutionary radiation enhanced by their isolation in South America during the Tertiary era, a period for which major climate variations and tectonic events are relatively well documented.     

Proliferative activity in the cerebellar external granular layer evaluated by bromodeoxyuridine labeling

We have optimised an indirect immunoperoxidase technique demonstrating bromodeoxyuridine (BrdU) incorporation into dividing cells for cerebellar tissue sections of four-day-old rats injected with this marker. This permits confident identification of granule-cell precursors engaged in DNA synthesis in the external granular layer of the developing cerebellum. Preservation of BrdU immunoreactivity is attained using methanol/acetic acid fixation and different pretreatments before immunostaining, while unlabeled nuclei can be recognized clearly after Feulgen or hematoxylin counterstaining. We established conditions to ensure satisfactory BrdU uptake without affecting cell-cycle progression during the postlabeling time period. The dose of BrdU employed provides saturation S-phase labeling from at least 1 h after BrdU delivery. Various kinetic parameters and phase durations have been determined in experiments involving a single injection or cumulative labeling sequences, and the cycle time was calculated based on two models of generative behavior: steady-state and exponential growth. The working hypothesis of steadystate kinetics can be adopted successfully if the existence of neuroblasts with different proliferation rates is taken into account.     

Endothelial cell junctions

In the course of a freeze-cleave study on intercellular junctions in the regenerating rat liver, we observed an unusual array of intramembranous particles located in regions of contact between endothelial cells lining the hepatic sinusoids. These arrays were characterized by an accumulation of particles which resembled a zonula occludens in their linear deployment but differed in that the contact regions were composed of individual particles which remained separated from each other by regular particle-free intervals.