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151.
152.
The 6-aminohexyl β-glycoside of N-acetylmuramyl-l-alanyl-d-isoglutamine and its spacer-arm-linked analog (3.8 nm) were synthesized from 2-methyl-(3,4,6-tri-O-acetyl-1,2-dideoxy-α-d-glucopyrano)-[2,1-d]-2-oxazoline, and coupled with meningococcal group C polysaccharide in attempts to enhance the immunogenicity of the polysaccharide antigen. 相似文献
153.
Bernal Len Silvia Fallas Gonzlez Lisa Miranda Solís Manuel Ramírez-Cardoce Andres Moreira-Soto Juan M. Cordero-Solrzano Sabine Elisabeth Hutter Rocío Gonzlez-Barrientos Charles E. Rupprecht 《The Yale journal of biology and medicine》2021,94(2):311
Rabies is an acute, progressive encephalitis caused by a lyssavirus, with the highest case fatality of any conventional infectious disease. More than 17 different lyssaviruses have been described, but rabies virus is the most widely distributed and important member of the genus. Globally, tens of thousands of human fatalities still occur each year. Although all mammals are susceptible, most human fatalities are caused by the bites of rabid dogs, within lesser developed countries. A global plan envisions the elimination of human rabies cases caused via dogs by the year 2030. The combination of prophylaxis of exposed humans and mass vaccination of dogs is an essential strategy for such success. Regionally, the Americas are well on the way to meet this goal. As one example of achievement, Costa Rica, a small country within Central America, reported the last autochthonous case of human rabies transmitted by a dog at the end of the 1970s. Today, rabies virus transmitted by the common vampire bat, Desmodus rotundus, as well as other wildlife, remains a major concern for humans, livestock, and other animals throughout the region. This review summarizes the historical occurrence of dog rabies and its elimination in Costa Rica, describes the current occurrence of the disease with a particular focus upon affected livestock, discusses the ecology of the vampire bat as the primary reservoir relevant to management, details the clinical characteristics of recent human rabies cases, and provides suggestions for resolution of global challenges posed by this zoonosis within a One Health context. 相似文献
154.
Sandra Barthel Gábor Bernát Tobias Seidel Eva Rupprecht Uwe Kahmann Dirk Schneider 《Plant physiology》2013,163(2):1037-1046
Thylakoid membranes are typical and essential features of both chloroplasts and cyanobacteria. While they are crucial for phototrophic growth of cyanobacterial cells, biogenesis of thylakoid membranes is not well understood yet. Dark-grown Synechocystis sp. PCC 6803 cells contain only rudimentary thylakoid membranes but still a relatively high amount of phycobilisomes, inactive photosystem II and active photosystem I centers. After shifting dark-grown Synechocystis sp. PCC 6803 cells into the light, “greening” of Synechocystis sp. PCC 6803 cells, i.e. thylakoid membrane formation and recovery of photosynthetic electron transport reactions, was monitored. Complete restoration of a typical thylakoid membrane system was observed within 24 hours after an initial lag phase of 6 to 8 hours. Furthermore, activation of photosystem II complexes and restoration of a functional photosynthetic electron transport chain appears to be linked to the biogenesis of organized thylakoid membrane pairs.Thylakoid membranes are typical and essential features of both chloroplasts and cyanobacteria. The intracellular thylakoid membranes of cyanobacteria harbor the protein complexes of the photosynthetic electron transport chain (Nowaczyk et al., 2010; Bernat and Rögner, 2011). The photosynthetic electron transport chain is composed of three large membrane protein complexes, i.e. PSII, the cytochrome b6f complex, and PSI. Excitation energy trapping by PSII results in water splitting at the PSII donor side within the thylakoid lumen and transport of electrons to the primary and secondary electron accepting quinone molecules QA and QB, respectively. Following double reduction and protonation, QB is released from PSII into the plastoquinone (PQ) pool and delivers electrons to the cytochrome b6f complex. The cytochrome b6f complex transfers the electrons to the soluble electron carrier plastocyanin or cytochrome c6, which subsequently reduces PSI. For efficient light harvesting, cyanobacteria contain soluble light-harvesting antenna proteins, the phycobilisomes (PBSs), which transfer light energy to the photosynthetic reaction centers. In cyanobacteria, the PSI-to-PSII ratio is controlled by light and by the redox state of the PQ/PQH2-pool (Fujita et al., 1987), and under high-light growth conditions, typically less PSI is present in cyanobacterial thylakoid membranes compared with low-light conditions (Fujita et al., 1994).Thylakoid membranes and photosynthetic electron transport are essential for phototrophic growth of cyanobacterial cells. Despite their importance for survival of cyanobacteria, biogenesis of thylakoid membranes is yet not well understood. It still is an ongoing debate whether the internal membrane systems (cytoplasmic and thylakoid membranes) are connected in cyanobacteria or not, and thus whether thylakoids represent a completely separated membrane entity (Liberton et al., 2006; van de Meene et al., 2006, 2012; Schneider et al., 2007). Up to now, only few proteins have been described to be involved in thylakoid membrane biogenesis. Among them the Vipp1 protein (vesicle inducing protein in plastids1) seems to play an important role in thylakoid membrane biogenesis, as in chloroplasts of Arabidopsis (Arabidopsis thaliana) and in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter referred to as Synechocystis), depletion of Vipp1 results in a reduced thylakoid membrane system (Kroll et al., 2001; Westphal et al., 2001). While the exact physiological function of the protein is not yet known (Vothknecht et al., 2012), depletion of Vipp1 in Synechocystis not only results in reduced thylakoid membrane formation, but also affects the activity and structure of components of the photosynthetic electron transport chain (Fuhrmann et al., 2009; Gao and Xu, 2009).As complexes of the respiratory electron transport chain are also localized in cyanobacterial thylakoids, the photosynthetic and respiratory electron transport pathways are highly interconnected and both contribute to formation of an electrochemical gradient across the thylakoid membrane and energy production. Due to this, Synechocystis is able to grow completely heterotrophically under light-activated photoheterotrophic growth (LAHG) conditions in the presence of high Glc concentrations (Anderson and McIntosh, 1991; Smart et al., 1991).In this study, we have used dark-grown Synechocystis cells to investigate “greening” of Synechocystis cells, i.e. thylakoid membrane formation and recovery of photosynthetic electron transport reactions. Following transfer of Synechocystis cells into the light, complete restoration of a typical thylakoid membrane system was observed within 24 h. While dark-grown Synechocystis cells contained only rudimentary thylakoid membranes, they still contained a high concentration of PBSs, active PSI as well as inactive PSII complexes. Activation of PSII complexes appears to be linked to the biogenesis of organized thylakoid membrane pairs. 相似文献
155.
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157.
Daniel G. Streicker Philippe Lemey Andres Velasco-Villa Charles E. Rupprecht 《PLoS pathogens》2012,8(5)
Rates of evolution span orders of magnitude among RNA viruses with important implications for viral transmission and emergence. Although the tempo of viral evolution is often ascribed to viral features such as mutation rates and transmission mode, these factors alone cannot explain variation among closely related viruses, where host biology might operate more strongly on viral evolution. Here, we analyzed sequence data from hundreds of rabies viruses collected from bats throughout the Americas to describe dramatic variation in the speed of rabies virus evolution when circulating in ecologically distinct reservoir species. Integration of ecological and genetic data through a comparative Bayesian analysis revealed that viral evolutionary rates were labile following historical jumps between bat species and nearly four times faster in tropical and subtropical bats compared to temperate species. The association between geography and viral evolution could not be explained by host metabolism, phylogeny or variable selection pressures, and instead appeared to be a consequence of reduced seasonality in bat activity and virus transmission associated with climate. Our results demonstrate a key role for host ecology in shaping the tempo of evolution in multi-host viruses and highlight the power of comparative phylogenetic methods to identify the host and environmental features that influence transmission dynamics. 相似文献
158.
Ivan V. Kuzmin Mang Shi Lillian A. Orciari Pamela A. Yager Andres Velasco-Villa Natalia A. Kuzmina Daniel G. Streicker David L. Bergman Charles E. Rupprecht 《PLoS pathogens》2012,8(6)
In nature, rabies virus (RABV; genus Lyssavirus, family Rhabdoviridae) represents an assemblage of phylogenetic lineages, associated with specific mammalian host species. Although it is generally accepted that RABV evolved originally in bats and further shifted to carnivores, mechanisms of such host shifts are poorly understood, and examples are rarely present in surveillance data. Outbreaks in carnivores caused by a RABV variant, associated with big brown bats, occurred repeatedly during 2001–2009 in the Flagstaff area of Arizona. After each outbreak, extensive control campaigns were undertaken, with no reports of further rabies cases in carnivores for the next several years. However, questions remained whether all outbreaks were caused by a single introduction and further perpetuation of bat RABV in carnivore populations, or each outbreak was caused by an independent introduction of a bat virus. Another question of concern was related to adaptive changes in the RABV genome associated with host shifts. To address these questions, we sequenced and analyzed 66 complete and 20 nearly complete RABV genomes, including those from the Flagstaff area and other similar outbreaks in carnivores, caused by bat RABVs, and representatives of the major RABV lineages circulating in North America and worldwide. Phylogenetic analysis demonstrated that each Flagstaff outbreak was caused by an independent introduction of bat RABV into populations of carnivores. Positive selection analysis confirmed the absence of post-shift changes in RABV genes. In contrast, convergent evolution analysis demonstrated several amino acids in the N, P, G and L proteins, which might be significant for pre-adaptation of bat viruses to cause effective infection in carnivores. The substitution S/T242 in the viral glycoprotein is of particular merit, as a similar substitution was suggested for pathogenicity of Nishigahara RABV strain. Roles of the amino acid changes, detected in our study, require additional investigations, using reverse genetics and other approaches. 相似文献
159.
Thylakoid Membrane Reduction Affects the Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp. PCC 6803
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Eva Fuhrmann Sven Gathmann Eva Rupprecht Jochen Golecki Dirk Schneider 《Plant physiology》2009,149(2):735-744
Biogenesis of thylakoid membranes in both chloroplasts and cyanobacteria is largely not understood today. The vesicle-inducing protein in plastids 1 (Vipp1) has been suggested to be essential for thylakoid membrane formation in Arabidopsis (Arabidopsis thaliana), as well as in the cyanobacterium Synechocystis sp. PCC 6803, although its exact physiological function remains elusive so far. Here, we report that, upon depletion of Vipp1 in Synechocystis cells, the number of thylakoid layers in individual Synechocystis cells decreased, and that, in particular, the content of photosystem I (PSI) complexes was highly diminished in thylakoids. Furthermore, separation of native photosynthetic complexes indicated that PSI trimers are destabilized and the monomeric species is enriched. Therefore, depletion of thylakoid membranes specifically affects biogenesis and/or stabilization of PSI in cyanobacteria.In chloroplasts and cyanobacteria the energy transfer between PSI and PSII is regulated in a light-dependent manner (for a recent review, see Kramer et al., 2004). The two photosystems are connected by the cytochrome b6f complex, and electron transfer from PSII via the cytochrome b6f complex to PSI is believed to be regulated by the redox state of the plastoquinol pool potentially also involving the cytochrome b6f complex (Fujita et al., 1987; Murakami and Fujita, 1993; Schneider et al., 2001, 2004; Pfannschmidt, 2003; Volkmer et al., 2007). Transfer of light energy to the two photosystems is mediated by light-harvesting complexes, and in cyanobacteria light is harvested by the soluble extramembranous phycobilisomes. The efficient energy transfer to PSI and PSII has to be balanced to synchronize the function of the two photosystems. In response to changing light intensities and qualities, energy coupling between the phycobilisomes and the photosystems changes, which allows a rapid adjustment of light absorbance by the individual photosystems. Furthermore, besides this short-term adaptation mechanism, it has been shown in many studies that on a longer term in cyanobacteria the ratio of the two photosystems changes depending on the light conditions (Manodori and Melis, 1986; Murakami and Fujita, 1993; Murakami et al., 1997). Upon shifting cyanobacterial cells from low-light to high-light growth conditions, the PSI-to-PSII ratio decreases due to selective suppression of the amount of functional PSI. In recent years, some genes have already been identified that are involved in this regulation of the photosystem stoichiometry (Hihara et al., 1998; Sonoike et al., 2001; Fujimori et al., 2005; Ozaki et al., 2007).Whereas in chloroplasts of higher plants and green algae the amounts of the two photosystems change in response to changing light conditions (Melis, 1984; Chow et al., 1990; Smith et al., 1990; Kim et al., 1993), it has already been noted a long time ago that the chloroplast ultrastructure also adapts to high-light and low-light conditions (Melis, 1984). Chloroplasts of plants grown under low light or far-red light have more thylakoid membranes than chloroplasts of plants grown under high light or blue light (Anderson et al., 1973; Lichtenthaler et al., 1981; Melis and Harvey, 1981). There appears to be a direct correlation between the chlorophyll content and the amount of thylakoids per chloroplast because light harvesting is increased by enhanced chlorophyll and thylakoid membrane content per chloroplast. Thus, chloroplasts adapt to high light both by a reduction of thylakoid membranes and by a decrease in the PSI-to-PSII ratio.Thylakoid membranes are exclusive features of both cyanobacteria and chloroplasts, and it still remains mysterious how formation of thylakoid membranes is organized. Many cellular processes, like lipid biosynthesis, membrane formation, protein synthesis in the cytoplasm and/or at a membrane, protein transport, protein translocation, and protein folding have to be organized and aligned for formation of internal thylakoid membranes. The recent observation that deletion of the vipp1 gene in Arabidopsis (Arabidopsis thaliana) results in complete loss of thylakoid membranes has indicated that Vipp1 is involved in biogenesis of thylakoid membranes. Further analysis has suggested that Vipp1 could be involved in vesicle trafficking between the inner envelope and the thylakoid membrane of chloroplasts (Kroll et al., 2001). Because of this, the protein was named Vipp1, for vesicle-inducing protein in plastids 1. Depletion of Vipp1 strongly affected the ability of cyanobacterial cells to form proper thylakoid membranes (Westphal et al., 2001) and, consequently, also in cyanobacteria Vipp1 appears to be involved in formation of thylakoid membranes. A Vipp1 depletion strain of Arabidopsis is deficient in photosynthesis, although the defect could not be assigned to a deficiency of a single photosynthetic complex, but appeared to be caused by dysfunction of the entire photosynthetic electron transfer chain (Kroll et al., 2001). Therefore, depletion of Vipp1 in Arabidopsis seems to affect thylakoid membrane formation rather than the assembly of thylakoid membrane protein complexes (Aseeva et al., 2007). However, for cyanobacteria, it is not clear yet how diminishing the amount of thylakoid membrane layers would affect the amount and stoichiometry of the two photosystems.Here, we present the generation and characterization of a Vipp1 depletion strain of the cyanobacterium Synechocystis sp. PCC 6803. Upon depletion of Vipp1, a decrease in thylakoid membrane pairs in the generated mutant strain and, furthermore, a significant decrease in active PSI centers was observed. Moreover, trimerization of PSI also appeared to be impaired in the mutant strain. These results suggest that thylakoid membrane perturbations caused by the Vipp1 depletion directly affects PSI assembly and stability in cyanobacterial thylakoid membranes. 相似文献