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821.
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Nicolai Miosge Werner G?tz Takako Sasaki Mon-Li Chu Rupert Timpl Rainer Herken 《The Histochemical journal》1996,28(2):109-116
Summary Fibulin-1 and fibulin-2, two recently identified extracellular matrix proteins with a homologous domain structure, are known
to bind various extracellular ligands and calcium. In this study, they have been localized at the light microscopical level
in human embryos of gestational weeks 4–10, using polyclonal antibodies. Identical localization patterns were observed for
the two fibulins in most of the tissues. In the heart, the endocardial cushion tissue and endocardium, but not the myocardium,
were stained, as were the basement membrane zones and adventitia of blood vessels. Staining was also observed in the perichondrium
and calcifying regions of developing bones. Moreover, reactions occurred with the gut subepithelium and epithelial basement
membranes of the skin. Differences in staining patterns, however, were observed in various neural structures. Fibulin-1 was
prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of
peripheral nerves. Fibulin-2 was detected primarily within the neuropithelium, spinal ganglia and peripheral nerves. The early
embryonic expression of both fibulins indicates specific roles during organ development and, in particular, involvement in
the differentiation of heart, skeletal and neuronal structures. 相似文献
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This study compared the effects of drug combinations commonly used for chemical restraint of southern elephant seals. The combinations were: ketamine and diazepam, ketamine and midazolam, ketamine and xylazine, and tiletamine and zolazepam. The main aims were to gather basic information regarding the response of the animals to the different combinations, and to determine which were most useful for routine chemical restraint. All drug combinations could be used safely although apnea and whole-body shaking occurred with each. There were significant differences in several of the responses measured. Poor muscle relaxation and prolonged apnea were associated with ketamine and diazepam use. Animals given ketamine and xylatine were more depressed, took longer to recover, had a higher incidence of thermoregulatory problems, and lower heart rate than after other combinations. Ketamine and midazolam and tiletamine and zolazepam produced fewer complications than the other drug combinations, and tiletamine and zolazepam showed greater predictability of response and ease of use, making it preferable for use by people with little experience in anesthesia of elephant seals. 相似文献
829.
Rupert Gerzer E. William Radany David L. Garbers 《Biochemical and biophysical research communications》1982,108(2):678-686
Two forms of soluble quanylate cyclase from mammalian tissues can be separated on DEAE Sephacel or Blue Sepharose CL-6B. The two forms, referred to as peak I or peak II, migrate identically during electrophoresis on polyacrylamide gels in the presence or absence of Na-dodecyl-SO4. Peak I is markedly stimulated by sodium nitroprusside and is the heme-containing form of guanylate cyclase. Peak II is only weakly stimulated by nitroprusside and contains no heme absorbance. In fresh tissue extracts, peak I is the predominant form, but it can be converted to peak II by treatments (pH 5.0, storage at 4°C) that result in the loss of the heme absorbance from the enzyme. Peak II is not formed from peak I by proteolysis. 相似文献
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