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61.
鄂西南香蒲科、黑三棱科、眼子菜科和茨藻科植物区系特征 总被引:2,自引:0,他引:2
本文在采集标本基础上,结合分析大量有关资料,讨论了鄂西南香蒲科等四科植物区系特征。结果表明:1.区系组成包括5属20种2变种1杂种。其中有1新种1新变种和3个湖北分布新记录。2.区系种类丰富,起源古老,对湖北同类区系具有代表性。3.有一定比例的特有成分(8.70%)。4.区系地理成分复杂多样,但主要具有东亚亚热带一温带分布(26.09%)和世界性分布(26.09%)的性质。5.本区四科植物区系主要与处于我国地貌第三级阶梯上的省区发生密切联系。其区系分区,在亚区的划分上与陆生种子植物相同。 相似文献
62.
本文报道了从我国手足口病(HFMD)患者疱液中肠道病毒71(E71)型H株的分离和鉴定。该株病毒可在原代人胚肺(HEL)细胞、MA104细胞、BSC细胞中生长繁殖,导致典型的肠道病毒CPE出现。将H株接种乳鼠后出现肢体麻痹、第6天开始死亡。电镜下可见感染H株的BSC细胞胞浆中出现大量的结晶状排列的成熟病毒颗粒,直径约25nm。患者双份血清中有对H株4倍增高的中和抗体存在。采用100抗体单位的抗Cox A5、7、9、16、E70、E71抗体和50抗体单位的LBM组合血清A-H以及抗E71BrCr株MeAb P27对H株进行中和试验时,H株可被抗E71血清和MeAb P27所中和。抗E71抗体对H株的最低有效中和作用为1.6抗体单位,MeAb P27对H株的有效中和作用是64抗体单位。其它血清则无此中和作用。然而,在鉴定过程中发现,高滴度的抗Cox A16抗体(200抗体单位以上)也显出有中和H株的作用,提示我们所分离的H株含有与Cox A16的型间共同抗原。 相似文献
63.
Evidence for two modes of Ca2+ entry following muscarinic stimulation of a human salivary epithelial cell line 总被引:1,自引:0,他引:1
Xinjun He Xiaozai Wu R. James Turner Bruce J. Baum 《The Journal of membrane biology》1990,115(2):159-166
Summary We have investigated muscarinic receptor-operated Ca2+ mobilization in a salivary epithelial cell line, HSG-PA, using an experimental approach which allows independent evaluation of intracellular Ca2+ release and extracellular Ca2+ entry. The carbachol (Cch) dose response of intracellular Ca2+ release indicates the involvement of a single, relatively low-affinity, muscarinic receptor site (K
0.510 or 30 m, depending on the method for [Ca2+]
i
determination). However, similar data for Ca2+ entry indicate the involvement of two Cch sites, one consistent with that associated with Ca2+ release and a second higher affinity site withK
0.52.5 m. In addition, the Ca2+ entry response observed at lower concentrations of Cch (2.5 m) was completely inhibited by membrane depolarization induced with high K+ (>55mm) or gramicidin D (1 m), while membrane depolarization had little or no effect on Ca2+ entry induced by 100 m Cch. Another muscarinic agonist, oxotremorine-M (100 m; Oxo-M), like Cch, also induced an increase in the [Ca2+]
i
of HSG-PA cells (from 72±2 to 104±5nm). This response was profoundly blocked (75%) by the inorganic Ca2+ channel blocker La3+ (25–50 m) suggesting that Oxo-M primarily mobilizes Ca2+ in these cells by increasing Ca2+ entry. Organic Ca2+ channel blockers (verapamil or diltiazem at 10 m, nifedipine at 1 m), had no effect on this response. The Oxo-M induced Ca2+ mobilization response, like that observed at lower doses of Cch, was markedly inhibited (70–90%) by membrane depolarization (high K+ or gramicidin D). At 100 m Cch the formation of inositol trisphosphate (IP3) was increased 55% above basal levels. A low concentration of carbachol (1 m) elicited a smaller change in IP3 formation (25%), similar to that seen with 100 m Oxo-M (20%). Taken together, these results suggest that there are two modes of muscarinic receptor-induced Ca2+ entry in HSG-PA cells. One is associated with IP3 formation and intracellular Ca2+ release and is independent of membrane potential; the other is less dependent on IP3 formation and intracellular Ca2+ release and is modulated by membrane potential. This latter pathway may exhibit voltage-dependent gating. 相似文献
64.
Bezold—Jarisch反射的生理机制与病理生理意义 总被引:5,自引:0,他引:5
Bezold-Jarisch反射是源于心脏内感受器的抑制性反射,其感受器主要分布在左心室,化学性和机械性刺激均可使之兴奋。感觉冲动经迷走神经无髓纤维上传,反射地引起交感传出活动减弱和迷走传出活动增强,终致心动徐缓和低血压。急性心肌梗塞、冠脉造影和劳力性晕厥时,有Bezold-Jarisch反射参与;慢性心衰和高血压时,心脏内感受器发生重调,反射的敏感性降低。 相似文献
65.
66.
EPR studies with 77Se-enriched (NiFeSe) hydrogenase of Desulfovibrio baculatus. Evidence for a selenium ligand to the active site nickel 总被引:5,自引:0,他引:5
S H He M Teixeira J LeGall D S Patil I Moura J J Moura D V DerVartanian B H Huynh H D Peck 《The Journal of biological chemistry》1989,264(5):2678-2682
The periplasmic hydrogenase containing equivalent amounts of nickel and selenium plus non-heme iron [NiFeSe) hydrogenase) has been purified from cells of the sulfate reducing bacterium Desulfovibrio baculatus (DSM 1748) grown on a lactate/sulfate medium containing natural Se isotopes and the nuclear isotope, 77Se. Both the 77Se-enriched and unenriched hydrogenases were shown to be free of other hydrogenases and characterized with regard to their Se contents. EPR studies of the reduced nickel signal generated by redox titrations of the enriched and unenriched (NiFeSe) hydrogenases demonstrated that the gx = 2.23 and gy = 2.17 resonances are appreciably broadened by the spin of the 77Se nucleus (I = 1/2). This observation demonstrates unambiguously that the unpaired electron is shared by the Ni and Se atoms and that Se serves as a ligand to the nickel redox center of the (NiFeSe) hydrogenase. 相似文献
67.
W W Vick J W Tello C J Wikstrand X He D Longee P Fredman L Svennerholm D D Bigner W W Johnston S H Bigner 《Acta cytologica》1992,36(5):697-705
The cytologic evaluation of poorly differentiated tumors frequently poses a diagnostic dilemma as to the tissue of origin. To assess the diagnostic utility of monoclonal antibodies (MAbs) in these situations, we applied a panel of three highly purified MAbs specific for tumor-associated ganglioside epitopes to a diverse series of cytologic specimens. The panel was composed of DMAb-3, reactive with the epitope GalNAc beta 1-4 (NeuAc alpha 2-3)Gal- of GM2; DMAb-7, reactive with the epitope (NeuAc alpha 2-8NeuAc alpha 2-3)Gal beta 1-4(Glc or GlcNAc)- of GD3 and 3'8'-LD1; and DMAb-20, reactive with the epitope GalNAc beta 1-4(NeuAc alpha 2-8NeuAc alpha 2-3)Gal- of GD2. The cytologic material consisted of air-dried Cytospin preparations prepared predominantly from fine needle aspirates and stained with the ABC immunohistochemical method. Positive reactivity was recognized when greater than 5% of tumor cells stained with the antibody; lesser reactivity was called negative. DMAb-3 stained 9/14 (64%) glial tumors, 4/13 (31%) nonglial central nervous system tumors, 1/21 (5%) melanomas, 7/38 (18%) non-small cell carcinomas (NSCC), 1/15 (7%) small cell carcinomas (SCC), 0/9 (0%) lymphomas/leukemias, 2/10 (20%) sarcomas, 1/7 (14%) miscellaneous tumors and 2/2 (100%) reactive fluids. DMAb-7 recognized 14/14 (100%) glial tumors, 9/13 (69%) non-glial central nervous system tumors, 19/22 (86%) melanomas, 19/43 (44%) NSCC, 5/15 (33%) SCC, 2/9 (22%) lymphomas/leukemias, 6/10 (60%) sarcomas, 1/7 (14%) miscellaneous tumors and 4/4 (100%) reactive fluids. DMAb-20 stained 6/14 (43%) glial tumors, 2/13 (15%) nonglial central nervous system tumors, 1/21 (5%) melanomas, 4/38 (10%) NSCC, 0/15 (0%) SCC, 0/9 (0%) lymphomas/leukemias, 1/10 (10%) sarcomas, 1/7 (14%) miscellaneous tumors and 1/3 (33%) reactive fluids. The GD3-reactive DMAb-7 recognized a large portion of many tumor types and thus is not diagnostically useful alone. DMAb-3 and DMAb-20 were more selective and showed the strongest reactivity for glial tumors and minimal reactivity for melanomas, small cell carcinomas, and lymphomas or leukemias. DMAb-3 and DMAb-20 may be useful as components of a larger panel of MAbs in distinguishing between poorly differentiated tumors in samples derived from the central nervous system. 相似文献
68.
黑龙江绥滨地区晚侏罗世东荣组的沟鞭藻类 总被引:12,自引:0,他引:12
本文对黑龙江绥滨86-11孔的沟鞭藻类进行了初步研究,共描述化石10属16种(包括2新种,6未定种);其中不少是在我国首次描述的。这些化石分成两个组合,其优势分子分别为Gonyaulacysta jurassica和Amphorula delicata。根据组合内各分子的时代分布,两化石组合的时代分别为Late Oxfordian—Early Kimmeridgian和Portlandian期,并对其产出层位东荣组的时代作了讨论,认为属于晚侏罗世。 相似文献
69.
蝌蚪(Bufo bufo andrewsi)血红细胞微核和核异常监测水质污染的研究 总被引:19,自引:2,他引:17
滇池是云南高原最大的淡水湖,是昆明地区工农业生产及人民生活用水的水源,又是昆明市的重要风景区。近年来,滇池水质污染,越来越引起人们的关注。昆明市的工业废水和生活污水经大观河等流入滇池,其中有毒有害物质多达30多种,包括镉、汞、砷、铅、铬、氟、有机磷、氰化物等。其中砷(Léonard et al., 1980;Katsuhiko, 相似文献
70.
四川青城山优食蚜蝇属二新种记述(双翅目:食蚜蝇科) 总被引:2,自引:0,他引:2
本文记述四川青城山优食蚜蝇属Eupeodes 2新种:青优食蚜蝇Eupeodes (Eupeodes) qingchengshanensis和小优食蚜蝇Eupeodes(Eupeodes)parvus,均属于Eupeodes s.str.,亚属。模式标本保存于上海农学院昆虫标本室。 相似文献