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The mitochondrion is a key organelle of eukaryotic cell that provides the energy for cellular activities. Correctly identifying submitochondria locations of proteins can provide plentiful information for understanding their functions. However, using web-experimental methods to recognize submitochondria locations of proteins are time-consuming and costly. Thus, it is highly desired to develop a bioinformatics method to predict the submitochondria locations of mitochondrion proteins. In this work, a novel method based on support vector machine was developed to predict the submitochondria locations of mitochondrion proteins by using over-represented tetrapeptides selected by using binomial distribution. A reliable and rigorous benchmark dataset including 495 mitochondrion proteins with sequence identity ≤25 % was constructed for testing and evaluating the proposed model. Jackknife cross-validated results showed that the 91.1 % of the 495 mitochondrion proteins can be correctly predicted. Subsequently, our model was estimated by three existing benchmark datasets. The overall accuracies are 94.0, 94.7 and 93.4 %, respectively, suggesting that the proposed model is potentially useful in the realm of mitochondrion proteome research. Based on this model, we built a predictor called TetraMito which is freely available at http://lin.uestc.edu.cn/server/TetraMito. 相似文献
954.
A modified Coomassie Brilliant Blue staining method at nanogram sensitivity compatible with proteomic analysis 总被引:4,自引:0,他引:4
A more sensitive and convenient Coomassie Brilliant Blue (CBB) staining method for visualizing proteins was developed. Compared with the modifications include the supplement of 10% (v/v) methanol into the fixing solution, an increase of an additional sensitization step and CBB raised from 0.1 to 0.125%. The improved method can detect proteins at nanogram level. The improved method is more sensitive than Blue Silver and more convenient than the Silver protocol. Mass spectrometry results confirmed that it is suitable for subsequent proteomic research. 相似文献
955.
Molecular phylogenetic analyses suggest an emerging phylogeny for the extant Placentalia (eutherian) that radically departs
from morphologically based constructions of the past. Placental mammals are partitioned into four supraordinal clades: Afrotheria,
Xenarthra, Laurasiatheria, and Euarchontoglires. Afrotheria form an endemic African clade that includes elephant shrews, golden
moles, tenrecs, aardvarks, hyraxes, elephants, dugongs, and manatees. Datamining databases of genome size (GS) shows that
till today just one afrotherian GS has been evaluated, that of the aardvark Orycteropus afer. We show that the GSs of six selected representatives across the Afrotheria supraordinal group are among the highest for
the extant Placentalia, providing a novel genomic signature of this enigmatic group. The mean GS value of Afrotheria, 5.3
± 0.7 pg, is the highest reported for the extant Placentalia. This should assist in planning new genome sequencing initiatives.
[Reviewing Editor: Dmitri Petrov] 相似文献
956.
Hung-Yen Hsieh Wen-Tseng Lo Don-Chung Liu Pei-Kai Hsu Wei-Cheng Su 《Environmental Biology of Fishes》2007,78(4):333-346
The aim of this study was to investigate the species composition and distribution of fish larvae in relation to hydrographic
conditions in the waters surrounding Taiwan Island (TI) in February 2003. In total, 242 kinds of fish larvae belonging to
127 genera and 75 families were recognized. Among these, 109 taxa were identified to the family or genus level, others to
the species level. The 12 predominant types, which constituted 71% of the total fish larvae, were Engraulis japonica, Scomber sp., Diaphus spp., Benthosema pterotum, Carangoides ferdau, Embolichthys mitsukurii, Maurolicus sp., unidentified Myctophidae, Gonostoma gracile, Trichiurus lepturus, unidentified Gobiidae, and Myctophum asperum. The distribution of fish larvae showed a clear association with water masses around TI, with higher abundances and lower
species richness northwest of TI where the China Coastal Current prevails, and lower abundances and higher species diversity
east of TI where the Kuroshio Current dominates. Cluster analysis distinguished three station groups and four species groups,
and the distribution patterns of fish larvae also corresponded to hydrographic conditions. The total abundances of fish larvae
and eight of the 12 predominant taxa showed significant and positive correlations with zooplankton abundance, which suggests
that food source might be a key factor determining the abundance and distribution of fish larvae during the winter. 相似文献
957.
The peregrine falcon (Falco peregrinus) population in southern Scandinavia was almost extinct in the 1970’s. A successful reintroduction project was launched in
1974, using captive breeding birds of northern and southern Scandinavian, Finnish and Scottish origin. We examined the genetic
structure in the pre-bottleneck population using eleven microsatellite markers and compared the data with the previously genotyped
captive breeding population and contemporary wild population. Museum specimens between 53 and 130 years old were analyzed.
Despite an apparent loss of historical genetic diversity, the contemporary population shows a relatively high level of genetic
variation. Considerable gene introgression from captive breeding stock used to repopulate the former range of southern Scandinavian
peregrines may have altered the genetic composition of this population. Both the historical and contemporary northern and
southern Scandinavian populations are genetically differentiated. The reintroduction project implemented in the region and
the use of non-native genetic stock likely prevented the southern Scandinavian population from extinction and thus helped
maintain the level of genetic diversity and prevent inbreeding depression. The population is rapidly increasing in numbers
and range and shows no indication of reduced fitness or adaptive capabilities in the wake of the severe bottleneck and the
reintroduction. 相似文献
958.
通过整理文献资料,依据Wilson 和Reeder (2005)兽类分类系统,共收集到新疆兽类138 种,分别隶属8目22 科。根据兽类在各个特定分布区的分布特点,采用Ward 的方法对各特定分布区进行聚类,依据特定分布区的相似性聚类结果,并结合自然地理条件,对新疆兽类动物地理分布格局进行了探讨,并将全疆兽类动物地理区划划分到第Ⅲ级。结果表明,物种丰富度与分布区的环境异质性密切相关,同时,区域环境一致性较高的地区,其兽类区系成份相似度亦较高,聚类结果的一致性较强;新疆兽类的分布受地形因素的影响较显著,天山和塔克拉玛干沙漠等明显的地理界限对兽类的交流、扩散起到明显的阻隔作用。 相似文献
959.
Montserrat Orencio-Trejo Susana De la Torre-Zavala Aida Rodriguez-Garcia Hamlet Avilés-Arnaut Argel Gastelum-Arellanez 《Bioenergy Research》2016,9(4):1023-1033
Plant biomass offers a renewable and environmentally favorable source of sugars that can be converted to different chemicals, second-generation ethanol, and other liquid fuels. Cellulose makes up approximately 45 % of the dry weight of lignocellulosic biomass. Prior to the enzymatic hydrolysis of cellulose, lignin and hemicellulose must be structurally altered or removed, at least in part, by chemical and/or physical pretreatments. However, the high cost and low efficiency of the enzymatic hydrolysis prevent the process from being economically competitive. For this reason, it is necessary to find enzymes suitable for this type of process, with higher specific activities and greater efficiency. Members of the Bacillus and Paenibacillus genera have been traditionally used for the production of many enzymes for industrial applications. Cellulases produced by both genera have shown activity on soluble and crystalline cellulose and high thermostability and/or activity over a wide pH spectrum. In this review, the most recent information about the characterization of cellulolytic enzymes obtained from new strains of the Bacillus and Paenibacillus genera are reviewed. We focused on the variety of isoenzymes produced by these cellulolytic strains, their optimal production and reaction conditions, and their kinetic parameters and biotechnological potential. 相似文献
960.