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51.
Mingjun Zheng Yuexin Hu Rui Gou Ouxuan Liu Xin Nie Xiao Li Qing Liu Yingying Hao Juanjuan Liu Bei Lin 《Journal of cellular and molecular medicine》2020,24(5):2819-2831
Ovarian carcinoma has the highest mortality among the malignant tumours in gynaecology, and new treatment strategies are urgently needed to improve the clinical status of ovarian carcinoma patients. The Cancer Genome Atlas (TCGA) cohort were performed to explore the immune function of the internal environment of tumours and its clinical correlation with ovarian carcinoma. Finally, four molecular subtypes were obtained based on the global immune-related genes. The correlation analysis and clinical characteristics showed that four subtypes were all significantly related to clinical stage; the immune scoring results indicated that most immune signatures were upregulated in C3 subtype, and the majority of tumour-infiltrating immune cells were upregulated in both C3 and C4 subtypes. Compared with other subtypes, C3 subtype had a higher BRCA1 mutation, higher expression of immune checkpoints, and optimal survival prognosis. These findings of the immunological microenvironment in tumours may provide new ideas for developing immunotherapeutic strategies for ovarian carcinoma. 相似文献
52.
Rui Zhou Huiying Sun Siting Zheng Jingwen Zhang Dongqiang Zeng Jianhua Wu Zhenhua Huang Xiaoxiang Rong Jianping Bin Yulin Liao Min Shi Wangjun Liao 《Journal of cellular and molecular medicine》2020,24(5):3229-3241
The heterogeneity in prognoses and chemotherapeutic responses of colon cancer patients with similar clinical features emphasized the necessity for new biomarkers that help to improve the survival prediction and tailor therapies more rationally and precisely. In the present study, we established a s troma-related l ncRNA s ignature (SLS) based on 52 lncRNAs to comprehensively predict clinical outcome. The SLS model could not only distinguish patients with different recurrence and mortality risks through univariate analysis, but also served as an independent factor for relapse-free and overall survival. Compared with the conventionally used TNM stage system, the SLS model clearly possessed higher predictive accuracy. Moreover, the SLS model also effectively screened chemotherapy-responsive patients, as only patients in the low-SLS group could benefit from adjuvant chemotherapy. The following cell infiltration and competing endogenous RNA (ceRNA) network functional analyses further confirmed the association between the SLS model and stromal activation-related biological processes. Additionally, this study also identified three phenotypically distinct colon cancer subtypes that varied in clinical outcome and chemotherapy benefits. In conclusion, our SLS model may be a significant determinant of survival and chemotherapeutic decision-making in colon cancer and may have a strong clinical transformation value. 相似文献
53.
FGFRL1 affects chemoresistance of small‐cell lung cancer by modulating the PI3K/Akt pathway via ENO1
Rui Chen Deyu Li Meng Zheng Bin Chen Ting Wei Yu Wang Man Li Weimei Huang Qin Tong Qi Wang Yaru Zhu Wei Fang Linlang Guo Shun Fang 《Journal of cellular and molecular medicine》2020,24(3):2123-2134
Fibroblast growth factor receptor‐like 1 (FGFRL1), a member of the FGFR family, has been demonstrated to play important roles in various cancers. However, the role of FGFRL1 in small‐cell lung cancer (SCLC) remains unclear. Our study aimed to investigate the role of FGFRL1 in chemoresistance of SCLC and elucidate the possible molecular mechanism. We found that FGFRL1 levels are significantly up‐regulated in multidrug‐resistant SCLC cells (H69AR and H446DDP) compared with the sensitive parental cells (H69 and H446). In addition, clinical samples showed that FGFRL1 was overexpressed in SCLC tissues, and high FGFRL1 expression was associated with the clinical stage, chemotherapy response and survival time of SCLC patients. Knockdown of FGFRL1 in chemoresistant SCLC cells increased chemosensitivity by increasing cell apoptosis and cell cycle arrest, whereas overexpression of FGFRL1 in chemosensitive SCLC cells produced the opposite results. Mechanistic investigations showed that FGFRL1 interacts with ENO1, and FGFRL1 was found to regulate the expression of ENO1 and its downstream signalling pathway (the PI3K/Akt pathway) in SCLC cells. In brief, our study demonstrated that FGFRL1 modulates chemoresistance of SCLC by regulating the ENO1‐PI3K/Akt pathway. FGFRL1 may be a predictor and a potential therapeutic target for chemoresistance in SCLC. 相似文献
54.
In recent years, plenty of studies found that circular RNAs (circRNAs) were essential players in the initiation and progression of various cancers including the renal cell carcinoma (RCC). However, the knowledge about the circRNAs in carcinogenesis is still limited. Dysregulated expression of circNUP98 in RCC tissues was identified by the circular RNA microarray. RT‐PCR was performed to measure the expression of circNUP98 in 78 pairs of RCC tissues and adjacent normal tissues. Survival analysis was conducted to explore the association between the expression of circNUP98 and the prognosis of RCC. The function and underlying mechanisms of circSMC3 in RCC cells were investigated by RNAi, CCK‐8, Western blotting, bioinformatic analysis, ChIP assay, circRIP assay and dual luciferase reporter assay. CircNUP98 was up‐regulated in both RCC tissues and cell lines, and high expression of circNUP98 was correlated with poor prognosis of RCC patients. Silencing of circSMC3 inhibited the proliferation and promoted the apoptosis in a caspase‐dependent manner in RCC cells. Mechanistically, we revealed that silencing of circ NUP98 inhibited RCC progression by down‐regulating of PRDX3 via up‐regulation of miR‐567. Furthermore, STAT3 was identified as an inducer of circ NUP98 in RCC cells. CircNUP98 acts as an oncogene by a novel STAT3/circ NUP98/miR‐567/PRDX3 axis, which may provide a potential biomarker and therapeutic target for the treatment of RCC. 相似文献
55.
Wenjing Yu Kaiju Sun Liying Zhang Xilin Wan Changbao Chen Rui Su Yifei Liu Hongfeng Wang Hongmei Yang 《化学与生物多样性》2020,17(8)
Squalene has been used as a dietary supplement for a long history due to its potential cancer‐preventive function. However, the mechanism has not been investigated in detail yet. Therefore, the aim of this study is to see if the plasma coenzyme Q10 (CoQ10) level will be altered by gavage of squalene and oxidosqualenes to rats. In the present work, a sensitive and simple high‐performance analytical method based on ultra‐high‐performance liquid chromatography coupled with an Orbitrap mass spectrometry (UPLC‐Orbitrap‐MS) was developed for the quantification of CoQ10 in rat plasma. Coenzyme Q9 (CoQ9) was employed as the internal standard. CoQ10 was determined after acetonitrile‐mediated plasma protein precipitation using UPLC‐Orbitrap‐MS in negative ion mode. Intragastric administration of squalene and the two squalene epoxides into rats once daily for several days elevated the level of CoQ10 in their plasma, but there was no significant difference between high‐dose (286 mg/kg) and low‐dose (143 mg/kg) groups. Intragastric administration of squalene once a day for 5 consecutive days and oxidosqualenes once a day for 3 consecutive days is necessary for reaching the steady‐state level of CoQ10. Our present findings indicate that squalene and oxidosqualenes may be useful for stimulating the synthesis of CoQ10 in rats. 相似文献
56.
Shasha Zhang Zonghao Liu Wenjun Zhang Zhaoyi Jiang Weitao Chen Rui Chen Yuqian Huang Zhichun Yang Yiqiang Zhang Liyuan Han Wei Chen 《Liver Transplantation》2020,10(35)
Perovskite solar cells (PSCs) have attracted much attention in the past decade and their power conversion efficiency has been rapidly increasing to 25.2%, which is comparable with commercialized solar cells. Currently, the long‐term stability of PSCs remains as a major bottleneck impeding their future commercial applications. Beyond strengthening the perovskite layer itself and developing robust external device encapsulation/packaging technology, integration of effective barriers into PSCs has been recognized to be of equal importance to improve the whole device’s long‐term stability. These barriers can not only shield the critical perovskite layer and other functional layers from external detrimental factors such as heat, light, and H2O/O2, but also prevent the undesired ion/molecular diffusion/volatilization from perovskite. In addition, some delicate barrier designs can simultaneously improve the efficiency and stability. In this review article, the research progress on barrier designs in PSCs for improving their long‐term stability is reviewed in terms of the barrier functions, locations in PSCs, and material characteristics. Regarding specific barriers, their preparation methods, chemical/photoelectronic/mechanical properties, and their role in device stability, are further discussed. On the basis of these accumulative efforts, predictions for the further development of effective barriers in PSCs are provided at the end of this review. 相似文献
57.
Ligang Wang Xinxuan Duan Xijun Liu Jing Gu Rui Si Yi Qiu Yaming Qiu Dier Shi Fanhong Chen Xiaoming Sun Jianhua Lin Junliang Sun 《Liver Transplantation》2020,10(4)
Water splitting requires development of cost‐effective multifunctional materials that can catalyze both the hydrogen evolution reaction (HER) and the oxygen evolution reaction (OER) efficiently. Currently, the OER relies on the noble‐metal catalysts; since with other catalysts, its operation environment is greatly limited in alkaline conditions. Herein, an advanced water oxidation catalyst based on metallic Co9S8 decorated with single‐atomic Mo (0.99 wt%) is synthesized (Mo‐Co9S8@C). It exhibits pronounced water oxidization activity in acid, alkali, and neutral media by showing positive onset potentials of 200, 90, and 290 mV, respectively, which manifests the best Co9S8‐based single‐atom Mo catalyst till now. Moreover, it also demonstrates excellent HER performance over a wide pH range. Consequently, the catalyst even outperforms noble metal Pt/IrO2‐based catalysts for overall water splitting (only requiring 1.68 V in acid, and 1.56 V in alkaline). Impressively, it works under a current density of 10 mA cm?2 with no obvious decay during a 24 h (0.5 m H2SO4) and 72 h (1.0 m KOH) durability experiment. Density functional theory (DFT) simulations reveal that the synergistic effects of atomically dispersed Mo with Co‐containing substrates can efficiently alter the binding energies of adsorbed intermediate species and decrease the overpotentials of the water splitting. 相似文献
58.
Changjian Zuo Zongxiang Hu Rui Qi Jiajie Liu Zhibo Li Junliang Lu Cheng Dong Kai Yang Weiyuan Huang Cong Chen Zhibo Song Sicheng Song Yaoming Yu Jiaxin Zheng Feng Pan 《Liver Transplantation》2020,10(34)
The relatively low capacity and capacity fade of spinel LiMn2O4 (LMO) limit its application as a cathode material for lithium‐ion batteries. Extending the potential window of LMO below 3 V to access double capacity would be fantastic but hard to be realized, as it will lead to fast capacity loss due to the serious Jahn–Teller distortion. Here using experiments combined with extensive ab initio calculations, it is proved that there is a cooperative effect among individual Jahn–Teller distortions of Mn3+O6 octahedrons in LMO, named as cooperative Jahn–Teller distortion (CJTD) in the text, which is the difficulty to access the capacity beyond one lithium intercalation. It is further proposed that the cationic disordering (excess Li at Mn sites and Li/Mn exchange) can intrinsically suppress the CJTD of Mn3+O6 octahedrons. The cationic disordering can break the symmetry of Mn3+ arrangements to disrupt the correlation of distortions arising from individual JT centers and prevent the Mn3+? O bonds distorting along one direction. Interestingly, with the suppressed CJTD, the original octahedral vacancies in spinel LMO are activated and can serve as extra Li‐ion storage sites to access the double capacity with good reversible cycling stability in microsized LMO. 相似文献
59.
Virologica Sinica - Coronavirus disease 2019 (COVID-19), reminiscent of the severe acute respiratory syndrome (SARS) outbreak in 2003, has been a tragic disaster to people all over the world. As... 相似文献
60.
为筛选对入侵害虫木瓜秀粉蚧毒力较好的农药及复配增效配比,为该虫的化学药剂防治提供理论依据,本文采用叶面喷雾法测定了11种农药对木瓜秀粉蚧2龄若虫的室内毒力,挑选毒力较好的两种药剂进行复配,筛选增效配比,并进行田间药效试验。结果表明,11种农药对木瓜秀粉蚧的毒力大小依次为:螺螨酯>哒螨灵>噻虫胺>联苯菊酯>矿物油>高效氯氰菊酯>炔螨特>啶虫脒>吡虫啉>噻螨酮>四螨嗪。哒螨灵与螺螨酯(60∶40)复配共毒系数最大,为182.47。田间药效试验发现,混配药剂防效均高于单剂且达到差异显著。 相似文献