全文获取类型
收费全文 | 12618篇 |
免费 | 1147篇 |
国内免费 | 4篇 |
出版年
2023年 | 45篇 |
2022年 | 98篇 |
2021年 | 209篇 |
2020年 | 101篇 |
2019年 | 160篇 |
2018年 | 179篇 |
2017年 | 164篇 |
2016年 | 344篇 |
2015年 | 542篇 |
2014年 | 694篇 |
2013年 | 791篇 |
2012年 | 1012篇 |
2011年 | 989篇 |
2010年 | 655篇 |
2009年 | 567篇 |
2008年 | 828篇 |
2007年 | 812篇 |
2006年 | 781篇 |
2005年 | 711篇 |
2004年 | 674篇 |
2003年 | 690篇 |
2002年 | 634篇 |
2001年 | 95篇 |
2000年 | 80篇 |
1999年 | 125篇 |
1998年 | 206篇 |
1997年 | 123篇 |
1996年 | 112篇 |
1995年 | 129篇 |
1994年 | 99篇 |
1993年 | 122篇 |
1992年 | 104篇 |
1991年 | 70篇 |
1990年 | 70篇 |
1989年 | 59篇 |
1988年 | 56篇 |
1987年 | 40篇 |
1986年 | 51篇 |
1985年 | 51篇 |
1984年 | 69篇 |
1983年 | 42篇 |
1982年 | 54篇 |
1981年 | 43篇 |
1980年 | 37篇 |
1979年 | 29篇 |
1978年 | 26篇 |
1977年 | 38篇 |
1976年 | 24篇 |
1974年 | 27篇 |
1973年 | 19篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
191.
Cyst hatching in Anostraca accelerated by retinoic acid,amplified by Calcium Ionophore A23187, and inhibited by Calcium-channel blockers 总被引:1,自引:1,他引:0
Cyst hatching, under standardized conditions, of the Anostracan species Thamnocephalus platyurus and Streptocephalus dichotomus was significantly accelerated but not increased by applying the morphogen retinoic acid (RA). Cyst hatching was enhanced but not accelerated by artificially increasing the inflow of Ca2+ to the embryonic cells, using Calcium Ionophore A 23 187. Cyst hatching was accelerated and amplified, to a level in excess of the summed effects of each treatment, by a combined application of RA and ionophore. It was inhibited almost quantitatively by the Calcium-channel blockers Nifedipin and Verapamil. The significance of these findings is discussed. 相似文献
192.
193.
Christine Dassargues Mamadou Sanou Grégoire Clerfayt Henry Naveau Edmond-Jacques Nyns 《Biotechnology letters》1992,14(12):1175-1180
Summary Anaerobic fermentation of wheat bran, a model solid substrate, was conducted under non-axenic conditions, in two reactors operated under different modes, all other conditions being strictly identical. The first reactor was a completely-mixed batch reactor. The second reactor was a percolator into which the liquid phase was recirculated in closed loop through the solid substrate acting as a stationary bed. The final yield of fermentation was obtained after 27 days in the completely-mixed batch reactor, and after 14 days only in the percolator. Scanning electron microscopy revealed that numerous micro-organisms adhered to the solid substrate acting as a carrier in the percolator, whereas only very few micro-organisms adhered to the solid substrate in the completely-mixed batch reactor. The results show that obtaining a durable direct contact between micro-organisms and their solid substrate improves the rate of solids degradation. 相似文献
194.
Christine Grosse 《Experimental & applied acarology》1992,15(1):v-v
Announcement from the. Publisher 相似文献
195.
Production of Cell Wall-Degrading Enzymes by the Phytopathogenic Fungus Sclerotinia sclerotiorum 总被引:2,自引:1,他引:1 下载免费PDF全文
The range of polysaccharide-degrading enzymes and glycosidases formed by the phytopathogenic fungus Sclerotinia sclerotiorum was monitored following growth on 16 carbohydrate substrates. Endo- and exoenzymes capable of degrading cellulosic, hemicellulosic, and pectinolytic polysaccharides were secreted. Pectinolytic activities were produced constitutively on all of the substrates tested. Cellulolytic enzymes were not induced in simple sugar (i.e., glucose or xylose) media. Polysaccharide growth substrates and cellulase inducers increased all of the enzyme activities tested. Gel filtration analysis revealed the appearance of new molecular forms of pectinase, β-xylosidase, and cellobiosidase during induction on pectin and carboxymethyl cellulose media. 相似文献
196.
Christine S. Evans Imelda M. Gallagher Peter T. Atkey David A. Wood 《Biodegradation》1991,2(2):93-106
The use of immunogold-cytochemical labelling techniques in electron microscopy of wood infected by basidiomycete fungi has assisted in the elucidation of the localisation of enzymes which degrade lignocellulose. The use of specific immunocytochemical techniques is discussed with respect to the authenticity and accuracy of the methods, the use of adequate controls in the gold-labelling procedure, and the immunospecificity of the antibodies.Localisation of the lignin-degrading enzymes, lignin-peroxidase and laccase, has shown that these enzymes do not bind to wood cell walls unless the process of decay has already commenced. Similarly localisation of cellulases Endoglucanase II (EGII) and Cellobiohydrolase I (CBHI) has shown that these enzymes only bind to exposed ends of cellulose fibrils and to partially degraded areas of the wood cell wall. -Glucosidase is always immobilised within the extracellular polysaccharide layer surrounding fungal hyphae.This review postulates that there is regulation of the release sequence of these lignocellulolytic enzymes defining the spatial arrangement between the hyphae and the wood cell wall. This hypothesis is presented diagrammatically. 相似文献
197.
Strictly anaerobic bacteria were enriched and isolated from freshwater sediment sources in the presence and absence of sulfate with sorbic acid as sole source of carbon and energy. Strain WoSo1, a Gram-negative vibrioid sulfate-reducing bacterium which was assigned to the species Desulfoarculus (formerly Desulfovibrio) baarsii oxidized sorbic acid completely to CO2 with concomitant stoichiometric reduction of sulfate to sulfide. This strain also oxidized a wide variety of fatty acids and other organic compounds. A Gram-negative rod-shaped fermenting bacterium, strain AmSo1, fermented sorbic acid stoichiometrically to about equal amounts of acetate and butyrate. At concentrations higher than 10 mM, sorbic acid fermentation led to the production of pentanone-2 and isopentanone-2 (3-methyl-2-butanone) as byproducts. Strain AmSo1 fermented also crotonate and 3-hydroxybutyrate to acetate and butyrate, and hexoses to acetate, ethanol, hydrogen, and formate. The guanine-plus-cytosine content of the DNA was 41.8±1.0 mol%. Sorbic acid at concentrations higher than 5 mM inhibited growth of this strain while strain WoSo1 tolerated sorbic acid up to 10 mM concentration. 相似文献
198.
The feeding habits of the filter-feeding fairy shrimp Streptocephalus proboscideus are documented experimentally by offering them ciliates, Volvox, rotifers, nematodes and small crustaceans as prey. Escape capabilities (e.g. swimming speed) rather than size or shape were found to determine these animals' vulnerability to predation by the fairy shrimp. Ingestion rates for Volvox increased hyperbolically with size and, at the high temperatures in which they live, fairy shrimps may daily remove the equivalent of their body weight from the environment. 相似文献
199.
A long-range restriction map of the human chromosome 19q13 region: Close physical linkage between CKMM and the ERCC1 and ERCC2 genes 总被引:12,自引:4,他引:8 下载免费PDF全文
200.
A locus for familial hypertrophic cardiomyopathy is closely linked to the cardiac myosin heavy chain genes, CRI-L436, and CRI-L329 on chromosome 14 at q11-q12 总被引:10,自引:1,他引:9 下载免费PDF全文
Scott D. Solomon Anja A. T. Geisterfer-Lowrance Hans-Peter Vosberg Gudrun Hiller John A. Jarcho Cynthia C. Morton Wesley O. McBride Anna L. Mitchell Allen E. Bale William J. McKenna J. G. Seidman Christine E. Seidman 《American journal of human genetics》1990,47(3):389-394
We report that a gene responsible for familial hypertrophic cardiomyopathy (HC) is closely linked to the cardiac alpha and beta myosin heavy chain (MHC) genes on chromosome 14q11. We have recently shown that probe CRI-L436, derived from the anonymous DNA locus D14S26, detects a polymorphic restriction fragment that segregates with familial HC in affected members of a large Canadian family. Using chromosomal in situ hybridization, we have mapped CRI-L436 to chromosome 14 at q11-q12. Because the cardiac MHC genes also map to this chromosomal band, we have determined the genetic distances between the cardiac beta MHC gene, D14S26, and the familial HC locus. Data presented here show that these three loci are linked within 5 centimorgans on chromosome 14 at q11-q12. The possibility that defects in either the cardiac alpha or beta MHC genes are responsible for familial HC is discussed. 相似文献