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11.
It was studied how temperature influences the NBD-Cl inactivation of sarcoplasmic reticulum Ca2+-ATPase and the protective effect of ATP under conditions preventing ATP hydrolysis. Two types of ATP-binding sites with Kd equal to 30 and 220 microM at 37 degrees C were found. ADP interacts with these sites with the (K'd = 20 and 200 microM). The temperature decrease from 25 degrees to 5 degrees C induces the abrupt increase in the Kd for the low affinity site. The possible reasons for heterogeneity of ATP-binding sites are discussed. The conclusion is made that interaction of monomers in oligomeric complex of Ca2+-ATPase induces heterogeneity of ATP-binding sites. 相似文献
12.
Goncharov Alexey I. Levina Inna S. Shliapina Viktoriia L. Morozov Ivan A. Rubtsov Petr M. Zavarzin Igor V. Smirnova Olga V. Shchelkunova Tatiana A. 《Biochemistry. Biokhimii?a》2021,86(11):1446-1460
Biochemistry (Moscow) - Progesterone and its synthetic analogues act on cells through different types of receptors, affecting proliferation and apoptosis. These compounds exert their effect through... 相似文献
13.
Russian Journal of Genetics - The study is focused on the analysis of the mechanisms underlying the formation and distribution of repeat clusters in mammalian chromosomes, as exemplified by a group... 相似文献
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Susanna-Assunta Sansone Daniel Schober Helen J. Atherton Oliver Fiehn Helen Jenkins Philippe Rocca-Serra Denis V. Rubtsov Irena Spasic Larisa Soldatova Chris Taylor Andy Tseng Mark R. Viant 《Metabolomics : Official journal of the Metabolomic Society》2007,3(3):249-256
In this article we present the activities of the Ontology Working Group (OWG) under the Metabolomics Standards Initiative
(MSI) umbrella. Our endeavour aims to synergise the work of several communities, where independent activities are underway
to develop terminologies and databases for metabolomics investigations. We have joined forces to rise to the challenges associated
with interpreting and integrating experimental process and data across disparate sources (software and databases, private
and public). Our focus is to support the activities of the other MSI working groups by developing a common semantic framework
to enable metabolomics-user communities to consistently annotate the experimental process and to enable meaningful exchange
of datasets. Our work is accessible via a public webpage and a draft ontology has been posted under the Open Biological Ontology
umbrella. At the very outset, we have agreed to minimize duplications across omics domains through extensive liaisons with
other communities under the OBO Foundry. This is work in progress and we welcome new participants willing to volunteer their
time and expertise to this open effort.
See the MSI Ontology Working Group website for a complete list of members and contributors. Web URL: 相似文献
16.
Denis V. Rubtsov Helen Jenkins Christian Ludwig John Easton Mark R. Viant Ulrich Günther Julian L. Griffin Nigel Hardy 《Metabolomics : Official journal of the Metabolomic Society》2007,3(3):223-229
The amount of data generated by NMR-based metabolomic experiments is increasing rapidly. Furthermore, diverse techniques increase
the need for informative and comprehensive meta-data. These factors present a challenge in the dissemination, interpretation,
reviewing and comparison of experimental results using this technology. Thus, there is a strong case for unification and standardisation
of the data representation for both academia and industry. Here, a systems analysis of an NMR-based metabolomics experiment
is presented in order to reveal the reporting requirements. An in-depth analysis of the NMR component of a metabolomics experiment
has been produced, and a first round of data standard development completed. This has focussed on both one- and two-dimensional
1H NMR experiments, but is also applicable to higher dimensions and other nuclei. We also report the modelling of this schema
using Unified Modelling Language (UML), and have extended this to a proof-of-concept implementation of the standard as an
XML schema. 相似文献
17.
Aleksei G. Menzorov Konstantin E. Orishchenko Veniamin S. Fishman Anastasia A. Shevtsova Roman V. Mungalov Inna E. Pristyazhnyuk Elena A. Kizilova Natalia M. Matveeva Natalia Alenina Michael Bader Nikolai B. Rubtsov Oleg L. Serov 《Journal of cellular biochemistry》2019,120(10):17208-17218
Neuronal tracing is a modern technology that is based on the expression of fluorescent proteins under the control of cell type–specific promoters. However, random genomic integration of the reporter construct often leads to incorrect spatial and temporal expression of the marker protein. Targeted integration (or knock-in) of the reporter coding sequence is supposed to provide better expression control by exploiting endogenous regulatory elements. Here we describe the generation of two fluorescent reporter systems: enhanced green fluorescent protein (EGFP) under pan-neural marker class III β-tubulin (Tubb3) promoter and mEos2 under serotonergic neuron-specific tryptophan hydroxylase 2 (Tph2) promoter. Differentiation of Tubb3-EGFP embryonic stem (ES) cells into neurons revealed that though Tubb3-positive cells express EGFP, its expression level is not sufficient for the neuronal tracing by routine fluorescent microscopy. Similarly, the expression levels of mEos2-TPH2 in differentiated ES cells was very low and could be detected only on messenger RNA level using polymerase chain reaction-based methods. Our data shows that the use of endogenous regulatory elements to control transgene expression is not always beneficial compared with the random genomic integration. 相似文献
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19.
The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae. 总被引:15,自引:36,他引:15 下载免费PDF全文
P M Rubtsov M M Musakhanov V M Zakharyev A S Krayev K G Skryabin A A Bayev 《Nucleic acids research》1980,8(23):5779-5794
The cloned 18 S ribosomal RNA gene from Saccharomyces cerevisiae have been sequenced, using the Maxam-Gilbert procedure. From this data the complete sequence of 1789 nucleotides of the 18 S RNA was deduced. Extensive homology with many eucaryotic as well as E. coli ribosomal small subunit rRNA (S-rRNA) has been observed in the 3'-end region of the rRNA molecule. Comparison of the yeast 18 S rRNA sequences with partial sequence data, available for rRNAs of the other eucaryotes provides strong evidence that a substantial portion of the 18 S RNA sequence has been conserved in evolution. 相似文献
20.
Murtazina DA Petukhov SP Rubtsov AM Storey KB Lopina OD 《Biochemistry. Biokhimii?a》2001,66(8):865-874
Although it was shown earlier that phosphorylation of Na,K-ATPase by cAMP-dependent protein kinase (PKA) occurs in intact cells, the purified enzyme in vitro is phosphorylated by PKA only after treatment by detergent. This is accompanied by an unfortunate side effect of the detergent that results in complete loss of Na,K-ATPase activity. To reveal the effect of Na,K-ATPase phosphorylation by PKA on the enzyme activity in vitro, the effects of different detergents and ligands on the stoichiometry of the phosphorylation and activity of Na,K-ATPase from duck salt glands (11-isoenzyme) were comparatively studied. Chaps was shown to cause the least inhibition of the enzyme. In the presence of 0.4% Chaps at 1 : 10 protein/detergent ratio in medium containing 100 mM KCl and 0.3 mM ATP, PKA phosphorylates serine residue(s) of the Na,K-ATPase with stoichiometry 0.6 mol Pi/mol of -subunit. Phosphorylation of Na,K-ATPase by PKA in the presence of the detergent inhibits the Na,K-ATPase. A correlation was found between the inclusion of Pi into the -subunit and the loss of activity of the Na,K-ATPase. 相似文献