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131.
A. P. Sinitsyn D. O. Osipov A. M. Rozhkova E. V. Bushina G. S. Dotsenko O. A. Sinitsyna E. G. Kondrat’eva I. N. Zorov O. N. Okunev V. A. Nemashkalov V. Yu. Matys A. V. Koshelev 《Applied Biochemistry and Microbiology》2014,50(8):761-772
Methods for the production and analysis of cellulase and hemicellulase enzyme preparations of various compositions based on the Penicillium verruculosum carbohydrase complex and intended for the effective hydrolysis of different types of cellulose-containing materials (CCMs) have been developed. New recombinant strains of P. verruculosum producing multienzyme carbohydrase complexes with increased activities of cellulases (due to the expression of endo-β-1,4-glucanases I and IV and cellobiohydrolase II from Trichoderma reesei) and hemicellulases (due to the expression of endo-β-1,4-xylanases from P. canescens and T. reesei and endo-β-1,4-mannanase from T. reesei) were constructed. The hydrolytic efficiency of the enzyme preparations (EPs) produced by the new recombinant strains during continuous hydrolysis of three CCM types (milled aspen, depitched pine wood, and milled bagasse) was studied. It was shown that new EPs containing recombinant proteins and retaining their own basic cellulase complex are characterized by the highest hydrolytic ability, exceeding that of the EP based on the original P. verruculosum strain. The recombinant enzyme preparations were highly stable; the optimal pH and temperature values for cellulase, xylanase and mannanase activities were in the range of 3.5–5.5 and 50–80°C, respectively. 相似文献
132.
Directional sensitivity of the abdominal giant fibers of the cricket to auditory stimulation has been investigated with special interest to the effect of the cerci position on the directional sensitivity of the giant neurons of the terminal abdominal ganglion. It has been shown that at least for some giant neurons the preferred directions of stimulation are practically independent of cerci position. (Fig. 2, 3). By other words these neurons have constant preferred directions in relation to the insect body, but not to the cerci. This property of giant neurons can be accounted for their changeable connections with many receptors having different directional selectivity. If innervation of the giant neuron is artificially restricted to one group of receptors with identical directional selectivity the described constancy of preferred directions in relation to the body disappears (Fig. 4). 相似文献
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P. V. Volkov A. M. Rozhkova M. V. Semenova I. N. Zorov A. P. Sinitsyn 《Biochemistry. Biokhimii?a》2013,78(10):1180-1189
Here we report the first isolation to homogeneous forms of two glucoamylases from the fungus Penicillium verruculosum and their study in comparison with known glucoamylases from Aspergillus awamori and Aspergillus niger. Genes that encode glucoamylases from P. verruculosum were cloned and expressed in the fungus Penicillium canescens, and the recombinant glucoamylases were obtained with subsequent study of their molecular weights, isoelectric points, optimal temperature and pH values, and stability. The catalytic activities of the recombinant glucoamylases were determined in relation to soluble potato starch. Changes in molecular mass distribution and content of low molecular weight products during starch hydrolysis by glucoamylases from P. verruculosum, A. awamori, and A. niger were studied. An exo-depolymerization mechanism was established to be the pathway for destruction of starch by the glucoamylases. 相似文献
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E. V. Bushina A. M. Rozhkova I. N. Zorov A. D. Satrutdinov A. O. Bekkarevich A. V. Koshelev O. N. Okunev A. P. Sinitsyn 《Applied Biochemistry and Microbiology》2012,48(5):493-499
Complex enzymatic preparations demonstrating activities homologous to pectinlyase A and heterologous to endo-1,4-β-glucanase from Penicilliumverruculosum and β-glycosidase from Aspergillusniger have been obtained on the basis of recombinant strains of the fungus Penicilliumcanescens. Two approaches were utilized: development of an enzymatic preparation on the basis of a new strain, which produced all three enzymes, and development of an enzymatic preparation via combined cultivation of three strains, each of which produced one of the enzymes. 相似文献