四种厌氧菌生化性状检测方法的比较

使用国内的三种厌氧菌生化鉴定装置及购进的API20A 条,对标准参考菌株10 株,临床分离株109 株及从粪便分离株 14 株共计 133 株进行了测试比较,虽然这四种方法各有其优缺点,但结果这几种方法与国外的API20A 法相比,其符合率及重复率均无明显差异(P> 0.05),而国内的厌氧菌生化鉴定装置都具有快速,简便、准确的特点,无论是符合率还是重复率都在80% 或90% 以上,当然这四种方法还存在着一定问题待改进,所以我们希望上述国内有关装置在不断地改进中完善,使我们的厌氧菌检测水平更上一层楼。     

单片机模拟心电图发生器的制作

本文介绍了采用８９Ｇ５１单片机的心电图信号发生器的原理和制作。本仪器小巧,灵便,能模拟产生标准件的十二导联心电图波形信号,可用来调整心电图仪和心电监视器的增益,走纸速度以及检查导联线,病人电极的性能。     

心室辅助径流泵的设计方法

我们正在研制一种心室辅助径流泵,可在体外循环中代替血泵使用,也可作为心衰病人短期心脏辅助泵使用。此种无密封件的径流的泵的轴尖支承结构由一根据氧化铝陶瓷制成的叶轮轴和两只氧化锆陶瓷制成的轴尖轴承组成。叶轮的外径为７２毫米,进流处直径为２４毫米。位于叶轮上表面的六个叶片的进流侧高度为５．５毫米。出流侧高度为３毫米。     

胃超声造影剂法对上消化道溃疡的诊断

目的：应用胃超声造影剂检测上消化溃疡。方法：采用“胃窗－８５超声造影剂化水量５００毫升口服后进行超声检查。结果：５６例超声检获胃溃疡２０例,十二指肠溃疡３６例。经手术病理,胃镜证实胃溃疡１７例,十二指肠溃疡３１例,Ｂ超误诊８例。Ｂ超诊断符合率分别赤８５％;８６％。结论：胃超声造影检查对上消化溃疡一定诊断价值     

大鼠面部伤害性刺激后纹状体边缘区内原癌基因c-fos和NOS的表达

为了研究纹状体边缘区和痛觉的关系,用c-fos和NADPH－d双标记方法研究了大鼠面部伤害性刺激后c-fos蛋白（Fos）和NOS在纹状体边缘区的表达。面部伤害性刺激后30分钟,边缘区中即出现Fos表达,刺激后3小时,Fos表达达最高峰,而且主要在边缘区部位表达。正常大鼠纹状体边缘区中有密集的NOS阳性神经元及纤维,面部伤害性刺激3小时后,纹状体其余部位的NOS阳性胞体及纤维减少或消失,但边缘区中仍保留,并可见少数Fos和NOS双标记细胞,提示纹状体边缘区可能和面部痛觉的调制有关。     

提高米索前列醇用于晚期妊娠引产的安全性研究

探讨提高米索前列醇用于晚期妊娠引产的安全性措施。方法：通过腹部检查,头盆评分、Ｂ超及胎心电子监护选择引产对象１２６例,随机分为两组,各６３例。２５微克组：米索前列醇２５微克阴道用药,２４小时重复一次,总量５０微克。５０微克组：米索前列醇５０微克阴道用药,２４小时重复一次,总量１００微克。     

冠心病患者运动致QRS延长的临床意义

为探讨冠心病病人由于急性心肌缺血对ＱＲＳ持续时间变化的影响及其意义。本文收集了５３例病人,均进行运动试验和冠脉造影,其中２０例经冠脉造影排除心病,其他３３例经冠脉造影确定为冠心病。结果运动使冠脉正常者ＱＲＳ持续时间缩短,而使冠心病者ＱＲＳ持续时间延长。     

Molecular Dynamics Study of Naturally Existing Cavity Couplings in Proteins

Couplings between protein sub-structures are a common property of protein dynamics. Some of these couplings are especially interesting since they relate to function and its regulation. In this article we have studied the case of cavity couplings because cavities can host functional sites, allosteric sites, and are the locus of interactions with the cell milieu. We have divided this problem into two parts. In the first part, we have explored the presence of cavity couplings in the natural dynamics of 75 proteins, using 20 ns molecular dynamics simulations. For each of these proteins, we have obtained two trajectories around their native state. After applying a stringent filtering procedure, we found significant cavity correlations in 60% of the proteins. We analyze and discuss the structure origins of these correlations, including neighbourhood, cavity distance, etc. In the second part of our study, we have used longer simulations (≥100ns) from the MoDEL project, to obtain a broader view of cavity couplings, particularly about their dependence on time. Using moving window computations we explored the fluctuations of cavity couplings along time, finding that these couplings could fluctuate substantially during the trajectory, reaching in several cases correlations above 0.25/0.5. In summary, we describe the structural origin and the variations with time of cavity couplings. We complete our work with a brief discussion of the biological implications of these results.     

线粒体损伤相关分子模式与创伤性败血症

创伤性败血症由于不易早期诊断且病情发展较快,因而具有较高的死亡率,其机制至今尚未完全明确。某些创伤性败血症患者虽长时间应用多种抗生素,却得不到预期的治疗效果。可见,有其他因素在创伤性败血症中发挥作用。研究发现,线粒体损伤相关分子模式(damage-associated molecular pattern,DAMP)与创伤性败血症有关。本文就线粒体损伤相关分子模式与创伤性败血症的关系进行综述。