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排序方式: 共有324条查询结果,搜索用时 250 毫秒
21.
Nesaei Abolfazl Naderi Ghale-noie Zari Khorshid Shamshiri Asma Afzaljavan Fahimeh Rivandi Mahdi Tajbakhsh Amir Homaei Shandiz Fatemeh Pasdar Alireza 《Molecular biology reports》2022,49(5):3549-3557
Molecular Biology Reports - Breast Cancer is the most frequent neoplasm diagnosed among women worldwide. Genetic background and lifestyle/environment play a significant role in the disease... 相似文献
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Cherkasov A Hsing M Zoraghi R Foster LJ See RH Stoynov N Jiang J Kaur S Lian T Jackson L Gong H Swayze R Amandoron E Hormozdiari F Dao P Sahinalp C Santos-Filho O Axerio-Cilies P Byler K McMaster WR Brunham RC Finlay BB Reiner NE 《Journal of proteome research》2011,10(3):1139-1150
Mortality attributable to infection with methicillin-resistant Staphylococcus aureus (MRSA) has now overtaken the death rate for AIDS in the United States, and advances in research are urgently needed to address this challenge. We report the results of the systematic identification of protein-protein interactions for the hospital-acquired strain MRSA-252. Using a high-throughput pull-down strategy combined with quantitative proteomics to distinguish specific from nonspecific interactors, we identified 13,219 interactions involving 608 MRSA proteins. Consecutive analyses revealed that this protein interaction network (PIN) exhibits scale-free organization with the characteristic presence of highly connected hub proteins. When clinical and experimental antimicrobial targets were queried in the network, they were generally found to occupy peripheral positions in the PIN with relatively few interacting partners. In contrast, the hub proteins identified in this MRSA PIN that are essential for network integrity and stability have largely been overlooked as drug targets. Thus, this empirical MRSA-252 PIN provides a rich source for identifying critical proteins essential for network stability, many of which can be considered as prospective antimicrobial drug targets. 相似文献
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Expression microarrays have great potential for clinical use but variability of the results represents a challenge for reliable
practical application. The amount of fluorescent dye used in microarray experiments is a significant source of variability
that has not been systematically studied. Here we demonstrate that the quantity of Cy3 dye affects microarray results performed
on tumor specimens. Signal-to-noise ratios and coefficients of variation are significantly improved by increasing Cy3 to 150–180
pmol, but any further increase does not improve the data. In conclusion, optimal amounts of dye reduce variability and improve
reliability of expression microarray experiments. 相似文献
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Naderi N Majidi M Mousavi Z Khoramian Tusi S Mansouri Z Khodagholi F 《Neurochemical research》2012,37(4):778-785
Most of the modulating effects of cannabinoids on pain are through putative cannabinoid CB1 and CB2 receptors. However, the
involvement of other receptors is also suggested. Cannabinoid compounds with analgesic activity such as palmitoylethanolamide
(PEA) show low affinity to CB1 and CB2 receptors, yet selectively activate GPR55 receptors. The objective of the present study
was to evaluate the possible role of spinal CB1 and GPR55 receptors on antinociceptive activity of PEA in formalin test as
well as in the spinal expression of IL1-β in rat. Intrathecal (i.t.) administration of PEA (1, 10 μg) significantly decreased
both pain-related scores in formalin test and IL1-β expression in rat spinal cord. Pretreatment of rats with low doses of
CB1 receptor antagonist/GPR55 receptor agonist AM251 (10, 100 ng; i.t.), did not attenuated the effect of PEA, yet even significantly
increased the effect of PEA on IL1-β expression in rat spinal cord. Interestingly, i.t. administration of low doses of AM251
per se significantly decreased both pain related behavior and spinal IL1-β expression in formalin test. These findings suggest
the possible involvement of receptors other than CB1 receptors in spinal pain pathways, such as GPR55, in pain modulating
activity of cannabinoids. 相似文献
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Meshram M Naderi S McConkey B Budman H Scharer J Ingalls B 《Biotechnology and bioengineering》2012,109(5):1193-1204
The production of biopharmaceuticals from mammalian cell culture is hindered by apoptosis, which is the primary cause of cell death in these cultures. As a tool for optimization of culture yield, this study presents a population-based model describing the progression of apoptosis in a monoclonal antibody (mAb)-producing Chinese hamster ovary (CHO) cell culture. Because mAb production does not cease when apoptosis begins, the model was designed to incorporate subpopulations at various stages in the progression of apoptosis. The model was validated against intracellular measurements of caspase activity as well as cell density, nutrient levels, and toxic metabolites. Since the specific details of apoptotic mechanisms have not been elucidated in this cell line, we employed a model comparison analysis that suggests the most plausible pathways of activation. 相似文献
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D. J. Burnett J. Khoo M. Naderi J. Y. Y. Heng G. D. Wang F. Thielmann 《AAPS PharmSciTech》2012,13(4):1511-1517
The aim of this study was to investigate the effect of processing route (i.e., quench cooling and ball milling) on the surface energy heterogeneity and surface chemistry of indomethacin (IMC). Recently developed inverse gas chromatography (IGC) methodology at finite concentrations was employed to determine the surface energy distributions of crystalline, quench cooled and milled IMC samples. Surface properties of crystalline and processed IMC were measurably different as determined by the IGC and other conventional characterization techniques: differential scanning calorimetry and powder X-ray diffraction. Quench cooled IMC was in fully amorphous form. Milled IMC showed no amorphous character by calorimetric or X-ray diffraction studies. It was demonstrated that both processed IMC samples were energetically more active than the crystalline IMC. In particular, milled IMC exhibited a relatively higher dispersive surface energy and higher surface basicity (electron donor capability). This may be attributed to the creation of surface defect sites or exposure of higher energy crystal facets during the milling process. This study confirms that processing route has notable influence on the surface energy distribution and surface acid–base character. IGC was demonstrated as a powerful technique for investigating surface properties of real-world, heterogeneous pharmaceutical materials.Key words: heterogeneity, indomethacin, inverse gas chromatography, surface disorder, surface energy 相似文献
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Mahinpour R Riazi G Shokrgozar MA Sarbolouki MN Ahmadian S Douraghi M Hadi Alijanvand H Azadmanesh K Heidari M Naghdi Gheshlaghi Z Moosavi-Movahedi AA 《Cell biology international》2012,36(4):403-408
Arsenical compounds exhibit a differential toxicity to cancer cells. Microtubules are a primary target of a number of anticancer drugs, such as arsenical compounds. The interaction of 1-NAA (1-naphthylarsonic acid) has been investigated on microtubule polymerization under in vitro and cellular conditions. Microtubules were extracted from sheep brain. Transmission electron microscopy was used to show microtubule structure in the presence of 1-NAA. Computational docking method was applied for the discovery of ligand-binding sites on the microtubular proteins. Proliferation of HeLa cells and HF2 (human foreskin fibroblasts) was measured by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay method following their incubation with 1-NAA. Fluorescence microscopic labelling was done with the help of α-tubulin monoclonal antibody and Tunel kit was used to investigate the apoptotic effects of 1-NAA on the HeLa cells. 1-NAA inhibits the tubulin polymerization by the formation of abnormal polymers having high affinity to the inner cell wall. 相似文献
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