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821.
822.
Spawning of the Australian freshwater fish Murray cod, Maccullochella peeli (Mitchell), in earthen ponds 总被引:2,自引:0,他引:2
S. J. Rowland 《Journal of fish biology》1983,23(5):525-534
Murray cod, Maccullochella peeli , spawned naturally in earthen ponds in four consecutive breeding seasons. Spawning was induced by a rise in water temperature up to or above 20°C during spring, however, an associated rise in water level was not required. Response to the temperature rise was more rapid later in the season and increasing daylength may have also been involved. Eggs were deposited on firm substrates at depths between 0·5 and 2·3 m, and hollow pipes, logs or similar structures were not necessary to provide suitable sites for egg deposition. At two spawning sites, mud had been removed from the pond banks by the broodfish and the eggs attached to the exposed clay. At one spawning site, a male cod was observed protecting the eggs during incubation.
It is suggested that high survival of cod larvae will only occur when a significant rise in water level coincides with the breeding season and as a consequence the control of water levels for irrigation and flood mitigation purposes during spring and summer has affected Murray cod to a greater extent than golden perch, Macauaria ambigua , which spawns only after a substantial rise in water level, when conditions are more favourable for larval survival. 相似文献
It is suggested that high survival of cod larvae will only occur when a significant rise in water level coincides with the breeding season and as a consequence the control of water levels for irrigation and flood mitigation purposes during spring and summer has affected Murray cod to a greater extent than golden perch, Macauaria ambigua , which spawns only after a substantial rise in water level, when conditions are more favourable for larval survival. 相似文献
823.
R. E. Rowland 《Experimental cell research》1980,126(2):444-448
In situ hybridization of labelled complementary RNA transcribed from whole DNA to metaphase chromosomes indicates the presence of repetitive DNA in both euchromatin and heterochromatin of the Vicia faba genome. 相似文献
824.
Eloise Rowland Peter Belton Paul W. Schaefer Gerhard Gries 《Physiological Entomology》2014,39(4):331-340
Tympanal ears of female gypsy moths Lymantria dispar dispar (L.) (Lepidoptera: Erebidae: Lymantriinae) are reportedly more sensitive than ears of conspecific males to sounds below 20 kHz. The hypothesis is tested that this differential sensitivity is a result of sex‐specific functional roles of sound during sexual communication, with males sending and females receiving acoustic signals. Analyses of sounds produced by flying males reveal a 33‐Hz wing beat frequency and 14‐kHz associated clicks, which remain unchanged in the presence of female sex pheromone. Females exposed to playback sounds of flying conspecific males respond with wing raising, fluttering and walking, generating distinctive visual signals that may be utilized by mate‐seeking males at close range. By contrast, females exposed to playback sounds of flying heterospecific males (Lymantria fumida Butler) do not exhibit the above behavioural responses. Laser Doppler vibrometry reveals that female tympana are particularly sensitive to frequencies in the range produced by flying conspecific males, including the 33‐Hz wing beat frequency, as well as the 7‐kHz fundamental frequency and 14‐kHz dominant frequency of associated clicks. These results support the hypothesis that the female L. dispar ear is tuned to sounds of flying conspecific males. Based on previous findings and the data of the present study, sexual communication in L. dispar appears to proceed as: (i) females emitting sex pheromone that attracts males; (ii) males flying toward calling females; and (iii) sound signals from flying males at close range inducing movement in females, which, in turn, provides visual signals that could orient males toward females. 相似文献
825.
826.
Phosphatases play an important role in cellular signaling networks by regulating the phosphorylation state of proteins. Phosphatases are classically considered to be promiscuous, acting on tens to hundreds of different substrates. We recently demonstrated that a shared phosphatase can couple the responses of two proteins to incoming signals, even if those two substrates are from otherwise isolated areas of the network. This finding raises a potential paradox: if phosphatases are indeed highly promiscuous, how do cells insulate themselves against unwanted crosstalk? Here, we use mathematical models to explore three possible insulation mechanisms. One approach involves evolving phosphatase KM values that are large enough to prevent saturation by the phosphatase’s substrates. Although this is an effective method for generating isolation, the phosphatase becomes a highly inefficient enzyme, which prevents the system from achieving switch-like responses and can result in slow response kinetics. We also explore the idea that substrate degradation can serve as an effective phosphatase. Assuming that degradation is unsaturatable, this mechanism could insulate substrates from crosstalk, but it would also preclude ultrasensitive responses and would require very high substrate turnover to achieve rapid dephosphorylation kinetics. Finally, we show that adaptor subunits, such as those found on phosphatases like PP2A, can provide effective insulation against phosphatase crosstalk, but only if their binding to substrates is uncoupled from their binding to the catalytic core. Analysis of the interaction network of PP2A’s adaptor domains reveals that although its adaptors may isolate subsets of targets from one another, there is still a strong potential for phosphatase crosstalk within those subsets. Understanding how phosphatase crosstalk and the insulation mechanisms described here impact the function and evolution of signaling networks represents a major challenge for experimental and computational systems biology. 相似文献
827.
Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the membrane by MinD. 相似文献
828.
The outer retina removes the first-order correlation, the background light level, and thus more efficiently transmits contrast.
This removal is accomplished by negative feedback from horizontal cell to photoreceptors. However, the optimal feedback gain
to maximize the contrast sensitivity and spatial resolution is not known. The objective of this study was to determine, from
the known structure of the outer retina, the synaptic gains that optimize the response to spatial and temporal contrast within
natural images. We modeled the outer retina as a continuous 2D extension of the discrete 1D model of Yagi et al. (Proc Int
Joint Conf Neural Netw 1: 787–789, 1989). We determined the spatio-temporal impulse response of the model using small-signal
analysis, assuming that the stimulus did not perturb the resting state of the feedback system. In order to maximize the efficiency
of the feedback system, we derived the relationships between time constants, space constants, and synaptic gains that give
the fastest temporal adaptation and the highest spatial resolution of the photoreceptor input to bipolar cells. We found that
feedback which directly modulated photoreceptor calcium channel activation, as opposed to changing photoreceptor voltage,
provides faster adaptation to light onset and higher spatial resolution. The optimal solution suggests that the feedback gain
from horizontal cells to photoreceptors should be ∼0.5. The model can be extended to retinas that have two or more horizontal
cell networks with different space constants. The theoretical predictions closely match experimental observations of outer
retinal function. 相似文献
829.
Susceptible C3H/He mice were immunized with the avirulent Corpus Christi strain of Trypanosoma cruzi and subsequently infected with virulent Brazil stain organisms. Seventy days after infection sera were isolated and analyzed on western blots of electrophoretically separated T. cruzi antigens prepared from culture-form parasites (primarily epimastigotes). More than 25 bands were identified. The antibodies were fractionated by elution from various regions of western blots corresponding to average molecular weights of approximately greater than 130, 77, 70, 60, 48, or 38 kDa. Each of these antibody preparations was then incubated with strips of nitrocellulose containing all of the electrophoretically separated T. cruzi, and cross-reactivity was determined. Antibodies isolated from the 130-, 77-, and 70-kDa regions all cross-reacted with each other. Antibodies eluted from the 60-kDa region bound antigens in the 60-, 70-, and the 77-kDa regions. More importantly, antibodies eluted from every region bound antigens in the 70-kDa region. Conversely, antibodies eluted from this region bound to antigens in all of the other regions. These data indicate the presence of (a) common antigenic epitope(s) in T. cruzi infections in these mice that is predominantly found in the 70-kDa antigen-antibody complex on western blots. 相似文献
830.
Species that form breeding aggregations, such as the three-spined stickleback, have ample opportunity to observe and interact with others in their respective populations. This exposes courting pairs to distraction or disruption from onlooking individuals. Thus, courting inconspicuously could be beneficial to males. We tested whether males would court inconspicuously by housing them in individual tanks with visual access to a neighbouring male. A dummy of a gravid female three-spined stickleback was presented to a male in the front quadrant of the tank while screens concealed either the front quarter or front half of the tank from the neighbour. When a portion of the tank was concealed from a neighbouring rival, males courted more in the concealed areas than those that were exposed to a rival. Hence, in the presence of rivals, males can adjust their courtship behaviour in ways that could increase their mating success. Whether this reflects an active ‘strategy’ or a passive result of the male being less distracted by its neighbour is presently unknown. 相似文献