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91.
Evolution of alpha 2-macroglobulin. The purification and characterization of a protein homologous with human alpha 2-macroglobulin from plaice (Pleuronectes platessa L.) plasma. 总被引:2,自引:2,他引:0 下载免费PDF全文
A papain-binding protein (PB-protein) was purified to homogeneity from the plasma of plaice (Pleuronectes platessa L.). PB-protein inhibited the activity of trypsin and pancreatic elastase (serine proteinases), thermolysin (a metalloproteinase) and papain (a cysteine proteinase). Presaturation of PB-protein with trypsin prevented the subsequent inhibition of thermolysin, and vice versa. Only catalytically active endopeptidases were bound by PB-protein. The catalytic activity of trypsin bound by PB-protein was inhibited by 95% against an insoluble protein substrate, but only by 38% against a low-molecular-weight synthetic substrate. The remaining activity of the bound trypsin was partially protected against further inhibition by soya-bean trypsin inhibitor. Trypsin bound by PB-protein showed a decrease of 67% in its reactivity with antibodies. The inhibitory activity of PB-protein was inactivated at pH 8.0 by methylamine (0.2M) or dithiothreitol (1 mM). The inhibition of proteinases by plaice PB-protein shows the distinctive characteristics of inhibition by human alpha 2-macroglobulin, and it is concluded that the plaice protein is a homologue of the human macroglobulin. 相似文献
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94.
Influence of Protein Synthesis on NO(3) Reduction, NH(4) Accumulation, and Amide Synthesis in Suspension Cultures of Paul's Scarlet Rose 下载免费PDF全文
Changes in the concentrations of NH4+ and amides during the growth of suspension cultures of rose (Rosa cv. Paul's Scarlet) cells were examined. When cells were grown in medium possessing only NO3− as a nitrogen source, the concentrations of NH4+ and amides increased to 4.0 × 10−1 and 5.9 micromoles per gram fresh weight, respectively. The amounts of both constituents declined during the later stages of growth. When a trace amount of NH4+ was added to the NO3− base starting medium, the concentration of NH4+ in the cells was increased to 7.0 × 10−1 micromoles per gram fresh weight. 相似文献
95.
The effect of neurotensin on submaximally-stimulated hepatobiliary and pancreatic secretion was studied in 6 healthy subjects. An intravenous infusion of neurotensin 1.4 ± 0.3 pmol/kg/min, designed to reproduce plasma neurotensin immunoreactivity levels within the physiological range, produced a significant increase in pancreatic bicarbonate output. Plasma concentrations of pancreatic polypeptide rose by 83 ± 16 pmol/l and were associated with a small reduction in trypsin, but no significant change in bilirubin outputs. 相似文献
96.
Madeleine Fletcher Philip T. LoVerde Charles S. Richards 《Experimental parasitology》1981,52(3):362-370
Individual adult Schistosoma mansoni from strains selected for high or low infectivity to specific strains of the snail intermediate host, Biomphalaria glabrata, were subjected to enzyme electrophoresis on starch gels. Fourteen enzyme systems were analyzed in an attempt to find electrophoretic markers associated with genes for infectivity to snails. The S. mansoni strains were selected from different isolates from Puerto Rico in several strains of B. glabrata. Of an estimated 18 loci, 3 were polymorphic and the remainder monomorphic. For 1 of the 3 polymorphic enzyme loci, lactate dehydrogenase (Ldh, EC 1.1.1.27), phenotype frequencies were correlated with infectivity to snails. In schistosome strains of low infectivity, frequencies of the Ldh-N phenotype ranged between 0.56 and 0.69, while in strains of high infectivity, Ldh-N frequencies were typically 0.91 to 1.00. Whether the correlation is accidental or due to some form of association, such as chromosomal linkage, between the locus responsible for variation in lactate dehydrogenase and a gene for infectivity to snails remains to be determined. 相似文献
97.
The depolarized forward-scattered light from solutions of rat tail collagen has been studied by photon correlation spectroscopy. The measured autocorrelation function is seen to decay on two widely different time scales. The decay time for the fast component is consistent with the rotational diffusion of rodlike collagen monomers. The slowly decaying autocorrelation component is attributed to large nonspecific aggregates of collagen. A substantial fraction of the collagen is in this aggregated form. Extrapolation of the faster decay times to zero concentration yields a value of θ = 1082 ± 30 sec?1 for the rotational diffusion coefficient of the collagen monomer. 相似文献
98.
K S Fletcher E G Bremer G A Schwarting 《The Journal of biological chemistry》1979,254(22):11196-11198
The neutral glycosphingolipid content of normal human erythrocytes was analyzed by a new method which utilizes high performance liquid chromatography. This rapid and accurate technique permits the quantitation of each of the major neutral glycolipids from individual blood samples. A correlation between the P blood group and the relative quantities of neutral glycosphingolipids is demonstrated. Erythrocytes from P1 individuals are shown to contain more globotriaosylceramide and less lactosylceramide than do erythrocytes from P2 individuals. The results of these experiments suggest the existence of a new phenotype in the P blood group system, and have further implications regarding the biosynthesis of the P blood group glycosphingolipids. 相似文献
99.
Estimated Drainage of Carbon from the Tricarboxylic Acid Cycle for Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose Cells 总被引:4,自引:3,他引:1 下载免费PDF全文
The amount of carbon (μmoles of carbon atoms) drained from the tricarboxylic acid cycle for protein synthesis was compared with μmoles of CO2 released from the cycle at 2-day intervals during the growth of suspension cultures of Paul's Scarlet rose. We concluded that during the period of most rapid protein synthesis (day 0-4) one-sixth as much carbon was drained from the tricarboxylic acid cycle for protein synthesis as was released as CO2. By day 8, one-thirtieth of the amount of carbon released as CO2 was incorporated into protein. Net protein synthesis stopped on day 8, but the evolution of CO2/culture continued at its maximum rate until day 10. 相似文献
100.
A rearranged chromosome 9 was found in 12 of 23 specimens of orangutan, 4 of Bornean and 8 of Sumatran origin. Nine animals were heterozygous, and 3 were homozygous carriers for the variant chromosome, which was also traced in 4 other animals not studied by us. This type of chromosome rearrangement has been previously described (Seuánez et al., 1976) and is probably the same chromosome shown by Lucas et al. (1973) and reported by Turleau et al. (1975) in other specimens. There is obviously a very high incidence of this variant chromosome 9 in Pongo pygmaeus, and it is unlikely that it could result from independent rearrangements occurring in unrelated specimens from two geographically isolated populations (Sumatran and Bornean). It is concluded that the rearrangement is of ancient origin and that it has been maintained in the populations of Pongo as a balanced polymorphism. This type of complex rearrangement resulting from two pericentric inversions, one inside the other, is compared with certain sporadic pericentric inversions in the human complement, with pericentric inversions which are polymorphic in other mammals, and with pericentric inversions involved in chromosome evolution in the Hominoidea. 相似文献