全文获取类型
收费全文 | 1948篇 |
免费 | 190篇 |
国内免费 | 1篇 |
出版年
2021年 | 26篇 |
2020年 | 16篇 |
2019年 | 29篇 |
2018年 | 21篇 |
2017年 | 23篇 |
2016年 | 31篇 |
2015年 | 63篇 |
2014年 | 79篇 |
2013年 | 116篇 |
2012年 | 84篇 |
2011年 | 83篇 |
2010年 | 52篇 |
2009年 | 49篇 |
2008年 | 71篇 |
2007年 | 62篇 |
2006年 | 72篇 |
2005年 | 59篇 |
2004年 | 54篇 |
2003年 | 42篇 |
2002年 | 54篇 |
2001年 | 71篇 |
2000年 | 35篇 |
1999年 | 29篇 |
1998年 | 28篇 |
1997年 | 20篇 |
1996年 | 16篇 |
1995年 | 21篇 |
1994年 | 18篇 |
1993年 | 16篇 |
1992年 | 41篇 |
1991年 | 34篇 |
1990年 | 25篇 |
1989年 | 30篇 |
1988年 | 35篇 |
1987年 | 40篇 |
1986年 | 34篇 |
1985年 | 34篇 |
1984年 | 27篇 |
1983年 | 30篇 |
1982年 | 28篇 |
1981年 | 15篇 |
1980年 | 16篇 |
1978年 | 29篇 |
1977年 | 19篇 |
1976年 | 24篇 |
1974年 | 29篇 |
1973年 | 24篇 |
1972年 | 16篇 |
1971年 | 21篇 |
1969年 | 16篇 |
排序方式: 共有2139条查询结果,搜索用时 15 毫秒
61.
62.
The sibling marine snails Littorina obtusata (L.) and Littorina mariae Sacchi & Rastelli are sympatrically distributed and the shells of both species are subject to similar breaking forces by predatory crabs. Nevertheless, the two species exhibit rather different growth and defence strategies. To determine growth patterns, we measured changes in five morphological variables with increasing shell length: body whorl thickness at the point of crushing force application, shell height (related to globosity), shell mass, body mass, and apertural lip thickness. We also measured ontogenetic changes in the ability to withstand shell crushing. For most morphological variables, L. mariae showed uniformly allometric growth of juveniles into adults. In contrast, L. obtusata usually exhibited a distinct change in growth pattern upon reaching maturity. As adults, L. mariae showed a more sustained increase in overall shell mass and in body whorl thickness (defence against crushing attacks) and also had proportionally thicker apertural lips (defence against peeling attacks). Littorina obtusata , however, grew to a larger size and their shells could accommodate larger bodies at all sizes. Furthermore, the strength of L. obtusata shells increased faster than could be accounted for by either overall shell mass or thickness at the point of force application, suggesting strengthening by other means such as changes in shell microstructure or shape (other than globosity). These results illustrate the viability of two contrasting antipredator strategies, despite a highly similar phylogenetic history and selective regime. 相似文献
63.
Mildly dyskaryotic smears are common, and women with such results are often followed up with further cervical smear tests. An important consideration in evaluating this practice would be the annual incidence of invasive cervical cancer. A reanalysis of five previous studies of the cytological follow up of women with mildly abnormal smear test results was undertaken to calculate this incidence. The annual incidence of invasive cancer in these women ranged from 0 to 420 per 100,000 women years. The large studies providing the most precise estimates had annual rates of 143 to 420 per 100,000 women years. This is 16 to 47 times greater than in women aged 15-34 years in England and Wales. The average rate was 208 per 100,000 women years. Women with mild dyskaryosis are at high risk of developing invasive cervical cancer despite cytological follow up. A full appraisal of the costs and benefits of colposcopy in this situation is urgently required. 相似文献
64.
D. Cherix D. J. C. Fletcher D. Chautems W. Fortelius G. Gris L. Keller R. Rosengren E. L. Vargo F. Walter 《Insectes Sociaux》1993,40(3):319-324
Summary Sexuals ofFormica lugubris fly to mating places, where females attract males by using a sex pheromone. Females collected on the nest surface before departing on a mating flight are much less attractive than those collected on the mating place after the mating flight, suggesting that the mating flight triggers the release of the sex pheromone. Olfactory cues are essential for males to locate females while they patrol. Males probably use visual cues to locate females once they have alighted nearby them. Males are also attracted by aggregations of other males on the ground, probably because one or several females are likely to be close to male aggregations. 相似文献
65.
Leckband D Chen YL Israelachvili J Wickman HH Fletcher M Zimmerman R 《Biotechnology and bioengineering》1993,42(2):167-177
The adhesion forces between various surfaces were measured using the "surface forces apparatus" technique. This technique allows for the thickness of surface layers and the adhesion force between them to be directly measured in controlled vapor or liquid environments. Three types of biological surfaces were prepared by depositing various lipid-protein monolayers (with thicknesses ranging from 1 to 4 nm) on the inert, molecularly smooth mica surface: (i) hydrophobic lipid monolayers; (ii) amphiphilic polyelectrolyte surfaces of adsorbed polylysine; and (iii) deposited bacterial S-layer proteins. The adhesion, swelling, and wetting properties of these surfaces was measured as a function of relative humidity and time. Initial adhesion is due mainly to the van der Waals forces arising from nonpolar (hydrophobic) contacts. Following adhesive contact, significant molecular rearrangements can occur which alter their hydrophobic-hydrophilic balance and increase their adhesion with time. Increased adhesion is generally enhanced by (i) increased relative humidity (or degree of hydration); (ii) increased contact time; and (iii) increased rates of separation. The results are likely to be applicable to the adhesion of many other biosurfaces, and show that the hydrophobicity of a lipid or protein surface is not an intrinsic property of that surface but depends on its environment (e.g., on whether it is in aqueous solution or exposed to the atmosphere), and on the relative humidity of the atmosphere. It also depends on whether the surface is in adhesive contact with another surface and-when considering dynamic (nonequilibrium) conditions-on the time and previous history of its interaction with that surface. (c) 1993 John Wiley & Sons, Inc. 相似文献
66.
Invasion of tissue culture cells by diarrhoeagenic strains of Escherichia coli which lack the enteroinvasive inv gene 总被引:2,自引:0,他引:2
Aberra Geyid Jon Fletcher Brehanu A. Gashe Åsa Ljungh 《FEMS immunology and medical microbiology》1996,14(1):15-24
Abstract Invasive Escherichia coli strains of certain serotypes invade by the same mechanism as the Shigella sp. It has been proposed that invasion of epithelial cells by EPEC strains may also occur; this is a previously overlooked property. In the present study E. coli strains isolated from patients with diarrhoea or ulcerative colitis, lacking the inv plasmid mediating classical invasion, but hybridizing with probes for different adhesins, were analyzed for their ability to invade HeLa and Caco-2 cells. The majority of strains invaded Caco-2 cells to a higher extent than HeLa cells. Adhesion to Caco-2 cells was a prerequisite for subsequent invasion of the cells but EAF, eae , EAgg and other known virulence factors were not sufficient to mediate invasion. In 8/9 E. coli strains invasion was enhanced after growth under iron restriction. Growth during anaerobic conditions did not influence subsequent invasion by E. coli strains whereas 6/9 strains had their invasive ability significantly decreased after growth in the presence of 1% glucose. The invasive process was inhibited by mannose but not by lactose, fucose or galactose. Our data indicate that strains of E. coli may invade Caco-2 cells by novel mechanisms which require adhesion to the cells but which differ from those of Salmonella sp., Yersinia sp., Shigella sp. and classical enteroinvasive E. coli . 相似文献
67.
C. Mark Fletcher David N. M. Jones Robert Diamond David Neuhaus 《Journal of biomolecular NMR》1996,8(3):292-310
Summary Two modifications to the commonly used protocols for calculating NMR structures are developed, relating to the treatment of NOE constraints involving groups of equivalent protons or nonstereoassigned diastereotopic protons. Firstly, a modified method is investigated for correcting for multiplicity, which is applicable whenever all NOE intensities are calibrated as a single set and categorised in broad intensity ranges. Secondly, a new set of values for pseudoatom corrections is proposed for use with calculations employing centre-averaging. The effect of these protocols on structure calculations is demonstrated using two proteins, one of which is well defined by the NOE data, the other less so. It is shown that failure to correct for multiplicity when using r-6 averaging results in overly precise structures, higher NOE energies and deviations from geometric ideality, while failure to correct for multiplicity when using r-6 summation can cause an avoidable degradation of precision if the NOE data are sparse. Conversely, when multiplicities are treated correctly, r-6 averaging, r-6 summation and centre averaging all give closely comparable results when the structure is well defined by the data. When the NOE data contain less information, r-6 averaging or r-6 summation offer a significant advantage over centre averaging, both in terms of precision and in terms of the proportion of calculations that converge on a consisten result.Abbreviations HMG
high mobility group
- NOE
nuclear Overhauser enhancement
- NOESY
nuclear Overhauser enhancement spectroscopy
- rmsd
root-mean-square deviation
- YASAP
yet another simulated-annealing protocol 相似文献
68.
A hierarchical response to differences in ration size in the reproductive performance of female three-spined sticklebacks 总被引:3,自引:0,他引:3
The response of female three-spined sticklebacks ( Gasterosteus aculeatus ) to differences in food ration during the breeding season was quantified for several variables related to reproductive performance. The measured protein and lipid contents (mg g−1 ) of eggs were unaffected by ration. Egg size increased with an increase in both ration and female size, but the proportional increase in egg size was much smaller than the proportional increase in ration. The best predictor of mean batch (clutch) fecundity and weight was female size. There was a small but significant increase in batch fecundity with ration, but the increase was not directly proportional to the increase in ration. The rate of spawning, total breeding season fecundity and total weight of eggs spawned over the breeding season were sensitive to ration with breeding season fecundity and weight increasing in direct proportion to ration. Thus, in female sticklebacks, there is a hierarchy of sensitivity to ration in the response of variables related to reproduction. 相似文献
69.
Autosomal dominant zonular cataract with sutural opacities localized to chromosome 17q11-12. 总被引:5,自引:0,他引:5
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
T Padma R Ayyagari J S Murty S Basti T Fletcher G N Rao M Kaiser-Kupfer J F Hejtmancik 《American journal of human genetics》1995,57(4):840-845
Congenital cataracts constitute a morphologically and genetically heterogeneous group of diseases that are a major cause of childhood blindness. Different loci for hereditary congenital cataracts have been mapped to chromosomes 1, 2, 16, and 17q24. We report linkage of a gene causing a unique form of autosomal dominant zonular cataracts with Y-sutural opacities to chromosome 17q11-12 in a three-generation family exhibiting a maximum lod score of 3.9 at D17S805. Multipoint analysis gave a 1-lod confidence interval of 17 cM. This interval is bounded by the markers D17S799 and D17S798, a region that would encompass a number of candidate genes including that coding for beta A3/A1-crystallin. 相似文献
70.
Resistance of Spiroplasma citri Lines to the Virus SVTS2 Is Associated with Integration of Viral DNA Sequences into Host Chromosomal and Extrachromosomal DNA
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Spiroplasmavirus SVTS2, isolated from Spiroplasma melliferum TS2, produces plaques when inoculated onto lawns of Spiroplasma citri M200H, a derivative of the type strain Maroc R8A2. S. citri strains MR2 and MR3, originally selected as colonies growing within plaques on a lawn of M200H inoculated with SVTS2, were resistant to SVTS2. Genomic DNA fingerprints and electrophoretic protein profiles of M200H, MR2, and MR3 were similar, but three proteins present in M200H were missing or significantly reduced in both resistant lines. None of these three polypeptides reacted with antiserum against S. citri membrane proteins, indicating that they probably are not surface-located virus receptors. Electroporation with SVTS2 DNA produced 1.5 x 10(sup5) transfectants per (mu)g of DNA in M200H but none in MR2 or MR3, suggesting that resistance may result from inhibition of viral replication. The digestion patterns of the extrachromosomal double-stranded (ds) DNA of these lines were similar. Three TaqI fragments of MR2 extrachromosomal DNA that were not present in M200H extrachromosomal DNA hybridized strongly to an SVTS2 probe, and two of these fragments plus an additional one hybridized with the MR3 extrachromosomal DNA, indicating that a fragment of SVTS2 DNA was present in the extrachromosomal ds DNA of MR2 and MR3 but not of M200H. When the restricted genomes of all three lines were probed with SVTS2 DNA, strong hybridization to two EcoRI fragments of chromosomal MR2 and MR3 DNA but not M200H DNA indicated that SVTS2 DNA had integrated into the genomes of MR2 and MR3 but not of M200H. When MR3 extrachromosomal ds DNA containing a 2.1-kb SVTS2 DNA fragment was transfected into M200H, the transformed spiroplasmas were resistant to SVTS2. These results suggest that SVTS2 DNA fragments, possibly integrated into the chromosomal or extrachromosomal DNA of a previously susceptible spiroplasma, may function as viral incompatibility elements, providing resistance to superinfection by SVTS2. 相似文献