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91.
92.
An efficient protocol was developed for in vitro clonal propagation of Plumbago zeylanica Linn. through nodal culture. Multiple shoots were induced from nodal explants of P. zeylanica on Murashige and Skoog's (1962) medium supplemented with 0.5 mg L–1 to 1.0 mg.L–1 6-benzyladenine and 3% (w/v) sucrose. Inclusion of IAA (0.01 mg L–1) in the culture medium improved the frequency of production of multiple shoots. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 0.25 mg L–1 IBA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil. 相似文献
93.
Somatic embryogenesis was achieved in callus cultures derived from immature cotyledonary explants ofHardwickia binata Roxb., a multipurpose leguminous tree, on semisolid modified Murashige and Skoog's (mMS) medium containing 2900 mg/l potassium nitrate (KNO3) supplemented with 4.64 µM kinetin (Kn) and 5.37µM a-naphthaleneacetic acid (NAA). Somatic embryos proliferated rapidly after transfer to MS basal medium supplemented with 2052.6 µM L-glutamine and 0.084 µM gibberellic acid (GA3). Maturation of somatic embryos was achieved on half-strength MS basal medium supplemented with 1.23 µM IBA and 2% (w/v) sucrose. Histological studies confirmed different developmental stages of somatic embryogenesis inHardwickia binata.
Abbreviations BA
N6-benzyladenine
- Kn
kinetin
- NAA
a-naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- GA3
gibberellic acid
- MS
Murashige and Skoog (1962) medium
- mMS
modified Murashige and Skoog (1962) medium 相似文献
94.
95.
B. Saha D. Swain P. Borgohain G. R. Rout H. Koyama S. K. Panda 《Plant biology (Stuttgart, Germany)》2020,22(4):701-708
- Worldwide, 50% of soil is acidic, which induces aluminium (Al) toxicity in plants, as the phyto‐availability of Al3+ increases in acidic soil. Plants responds to Al3+ toxicity by exuding organic acids into the rhizosphere. The organic acid responsible for Al3+ stress response varies from species to species, which in the case of blackgram (Vigna mungo L.) is citrate.
- In blackgram, an Arabidopsis malate transporter, AtALMT1, was overexpressed with the motive of inducing enhanced exudation of malate. Transgenics were generated using cotyledon node explants through Agrobacterium tumefaciens‐mediated transformation. The putative transgenics were initially screened by AtALMT1‐specific genomic DNA PCR, followed by quantitative PCR. Two independent transgenic events were identified and functionally characterized in the T3 generation.
- The transgenic lines, Line 1 and 2, showed better root growth, relative water content and chlorophyll content under Al3+ stress. Both lines also accounted for less oxidative damage, due to reduced accumulation of ROS molecules. Photosynthetic efficiency, as measured in terms of Fv/Fm, NPQ and Y(II), increased when compared to the wild type (WT). Relative expression of genes (VmSTOP1, VmALS3, VmMATE) responsible for Al3+ stress response in blackgram showed that overexpression of a malate transporter did not have any effect on their expression. Malate exudation increased whereas citrate exudation did not show any divergence from the WT. A pot stress assay found that the transgenics showed better adaptation to acidic soil.
- This report demonstrates that the overexpression of a malate transporter in a non‐malate exuding species improves adaptation to Al3+ toxicity in acidic soil without effecting its stress response mechanism.
96.
The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly 下载免费PDF全文
Niepel M Strambio-de-Castillia C Fasolo J Chait BT Rout MP 《The Journal of cell biology》2005,170(2):225-235
The two yeast proteins Mlp1p and Mlp2p (homologues of the vertebrate protein Tpr) are filamentous proteins attached to the nuclear face of nuclear pore complexes. Here we perform a proteomic analysis, which reveals that the two Mlps have strikingly different interacting partners, testifying to their different roles within the cell. We find that Mlp2p binds directly to Spc110p, Spc42p, and Spc29p, which are three core components of the spindle pole body (SPB), the nuclear envelope-associated yeast spindle organizer. We further show that SPB function is compromised in mlp2 mutants. Cells lacking Mlp2p form significantly smaller SPBs, accumulate aberrant SPB component-containing structures inside the nucleus, and have stochastic failures of cell division. In addition, depletion of Mlp2p is synthetically lethal with mutants impaired in SPB assembly. Based on these data, we propose that Mlp2p links the SPB to the peripheral Mlp assembly, and that this linkage is required for efficient incorporation of components into the SPB. 相似文献
97.
Tackett AJ Dilworth DJ Davey MJ O'Donnell M Aitchison JD Rout MP Chait BT 《The Journal of cell biology》2005,169(1):35-47
We have dissected specialized assemblies on the Saccharomyces cerevisiae genome that help define and preserve the boundaries that separate silent and active chromatin. These assemblies contain characteristic stretches of DNA that flank particular regions of silent chromatin, as well as five distinctively modified histones and a set of protein complexes. The complexes consist of at least 15 chromatin-associated proteins, including DNA pol epsilon, the Isw2-Itc1 and Top2 chromatin remodeling proteins, the Sas3-Spt16 chromatin modifying complex, and Yta7, a bromodomain-containing AAA ATPase. We show that these complexes are important for the faithful maintenance of an established boundary, as disruption of the complexes results in specific, anomalous alterations of the silent and active epigenetic states. 相似文献
98.
Tackett AJ DeGrasse JA Sekedat MD Oeffinger M Rout MP Chait BT 《Journal of proteome research》2005,4(5):1752-1756
Isolation of protein complexes via affinity-tagged proteins provides a powerful tool for studying biological systems, but the technique is often compromised by co-enrichment of nonspecifically interacting proteins. We describe a new technique (I-DIRT) that distinguishes contaminants from bona fide interactors in immunopurifications, overcoming this most challenging problem in defining protein complexes. I-DIRT will be of broad value for studying protein complexes in biological systems that can be metabolically labeled. 相似文献
99.
Somatic embryogenesis was achieved in callus cultures dervied from 40-day-old semimature zygotic embryos ofDalbergia sissoo on semi-solid Murashige and Skoog (MS) salts and vitamins supplemented with 0.46–1.16 M kinetin, 6.78–9.04 M 2,4-dichlorophenoxy acetic acid (2,4-D) and 30 g/1 sucrose. Somatic embryos proliferated rapidly by secondary somatic embryogenesis after transfer to half-strength basal MS medium supplemented with 0.46-1.16 M kinetin and 6.78–9.04 M 2,4-D with 2% (w/v) sucrose. The light-green somatic embryos germinated on half-strength MS salts and vitamins supplemented with 0.5 mg/1 abscisic acid and 2% (w/v) sucrose. The developmental stages of somatic embryogenesis were studied by light and scanning electron microscopy.Abbreviations
ABA
Abscisic acid
-
BA
6-benzyladenine
-
Kn
kinetin
- 2,4-D
2,4-dichlorophenoxyacetic acid
-
NAA
1-naphthaleneacetic acid
-
MS
Murashige and Skoog (1962) basal medium 相似文献
100.
Gyana Ranjan Rout Sanghamitra Samantaray Premananda Das 《Acta Physiologiae Plantarum》1998,20(3):269-275
Nickel tolerant callus lines of Setaria italica L. were developed from callus cultures grown on MS medium supplemented with 0.5 mg·dm−3 kinetin+2.0 mg·dm−3 2,4-D+2.0 mg·dm−3 Ni+2. Standard growth parameters such as callus fresh and dry weight, growth tolerance index were used as indicators of nickel
toxicity. Measurements as early as 2 weeks after the beginning of the treatments did not yield consistent results. However,
growth tolerance index at 4, and 8 weeks after the beginning of treatments yielded significant differences among the non-tolerant
and tolerant calli. The tolerant calli has enhanced growth at 2.0 mg·dm−3 Ni+2 while non-tolerant calli showed a reverse trend in growth in the presence of 2.0–2.5 mg·dm−3 of nickel. The tolerant calli differentiated into mass of embryogenic calli within 4 weeks of culture which could be maintained
for prolonged period without loss of regenerative capacity. 相似文献